(Circular) (Six-base) MAPSORT of: pFN21AB7440.seq Check: 3434 from: 1 to: 5161 pFN21AB7440 5161 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2971 278 Size: 53 83 186 936 1435 2468 Fragments arranged by size: 2468 1435 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2504 1937 Size: 567 4594 Acc65I G'GTAC_C Cuts at: 2482 2482 Size: 5161 AclI AA'CG_TT Cuts at: 1563 3288 3661 1563 Size: 1725 373 3063 Fragments arranged by size: 3063 1725 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 3224 4608 4876 1632 Size: 704 888 1384 268 1917 Fragments arranged by size: 1917 1384 888 704 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4964 AflIII A'CryG_T Cuts at: 1203 2373 4060 5037 1203 Size: 1170 1687 977 1327 Fragments arranged by size: 1687 1327 1170 977 AgeI A'CCGG_T Cuts at: 3992 3992 Size: 5161 AhdI GACnn_n'nnGTC Cuts at: 3890 3890 Size: 5161 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5161 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2421 1184 Size: 1237 3924 AlwNI CAG_nnn'CTG Cuts at: 2904 4476 2904 Size: 1572 3589 ApaLI G'TGCA_C Cuts at: 3218 4374 3218 Size: 1156 4005 ApoI r'AATT_y Cuts at: 1493 2470 2696 2848 4957 1493 Size: 977 226 152 2109 1697 Fragments arranged by size: 2109 1697 977 226 152 AseI AT'TA_AT Cuts at: 160 1032 3715 160 Size: 872 2683 1606 Fragments arranged by size: 2683 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5161 AvaI C'yCGr_G Cuts at: 1870 1943 2151 2486 1870 Size: 73 208 335 4545 Fragments arranged by size: 4545 335 208 73 BamHI G'GATC_C Cuts at: 2491 2858 2491 Size: 367 4794 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2147 2482 3937 618 Size: 325 217 627 360 335 1455 1842 Fragments arranged by size: 1842 1455 627 360 335 325 217 BanII G_rGCy'C Cuts at: 1391 2394 2480 1391 Size: 1003 86 4072 Fragments arranged by size: 4072 1003 86 BbeI G_GCGC'C Cuts at: 2151 2151 Size: 5161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3352 3386 1903 Size: 34 1415 34 3678 Fragments arranged by size: 3678 1415 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3053 4263 3053 Size: 1210 3951 BclI T'GATC_A Cuts at: 1548 1548 Size: 5161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3772 136 Size: 107 122 71 631 605 2100 1525 Fragments arranged by size: 2100 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5156 5156 Size: 5161 BlpI GC'TnA_GC Cuts at: 2578 2578 Size: 5161 Bme1580I G_kGCm'C Cuts at: 1790 2446 3222 4378 1790 Size: 656 776 1156 2573 Fragments arranged by size: 2573 1156 776 656 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2892 3850 442 Size: 416 53 83 399 24 1475 958 1753 Fragments arranged by size: 1753 1475 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 3821 1239 Size: 1031 1551 2579 Fragments arranged by size: 2579 1551 1031 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2069 3286 4151 4449 2069 Size: 1217 865 298 2781 Fragments arranged by size: 2781 1217 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3824 882 Size: 617 2325 2219 Fragments arranged by size: 2325 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2447 2952 493 Size: 711 1243 505 2702 Fragments arranged by size: 2702 1243 711 505 BsaBI GATnn'nnATC Cuts at: 5155 5155 Size: 5161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2968 3350 3985 Size: 53 83 186 936 615 820 382 635 Cuts at: 3985 275 Size: 1451 Fragments arranged by size: 1451 936 820 635 615 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3594 3992 4266 4413 4824 3594 Size: 398 274 147 411 3931 Fragments arranged by size: 3931 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2279 2309 1178 Size: 30 165 30 876 30 4030 Fragments arranged by size: 4030 876 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5161 BseYI C'CCAG_C Cuts at: 2156 2173 4364 4860 2156 Size: 17 2191 496 2457 Fragments arranged by size: 2457 2191 496 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4588 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2611 3372 4400 1540 Size: 275 186 610 761 1028 2301 Fragments arranged by size: 2301 1028 761 610 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 3222 3307 4378 2394 Size: 828 85 1071 3177 Fragments arranged by size: 3177 1071 828 85 BsiWI C'GTAC_G Cuts at: 2073 2073 Size: 5161 BsmI GAATG_Cn' Cuts at: 2672 2765 2672 Size: 93 5068 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2446 2480 3222 3307 4378 1391 Size: 399 604 52 34 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 399 85 52 34 BspEI T'CCGG_A Cuts at: 4824 4824 Size: 5161 BspHI T'CATG_A Cuts at: 3050 4780 3050 Size: 1730 3431 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2516 844 Size: 1672 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3048 1394 Size: 429 1225 3507 Fragments arranged by size: 3507 1225 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3656 3830 65 Size: 1183 2408 174 1396 Fragments arranged by size: 2408 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2144 3805 3992 1700 Size: 110 334 1661 187 2869 Fragments arranged by size: 2869 1661 334 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5161 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5161 BssSI C'ACGA_G Cuts at: 3221 4233 3221 Size: 1012 4149 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5161 BstBI TT'CG_AA Cuts at: 3965 3965 Size: 5161 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5161 BstXI CCAn_nnnn'nTGG Cuts at: 2439 2439 Size: 5161 BstYI r'GATC_y Cuts at: 1972 2491 2858 3243 3260 4712 4798 4810 5156 Size: 519 367 385 17 1452 86 12 346 Cuts at: 5156 1972 Size: 1977 Fragments arranged by size: 1977 1452 519 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2202 513 Size: 552 939 198 3472 Fragments arranged by size: 3472 939 552 198 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4422 Fragments arranged by size: 4422 683 56 BtsI GCAGTG_nn' Cuts at: 2677 3471 3491 2677 Size: 794 20 4347 Fragments arranged by size: 4347 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5161 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5126 DraI TTT'AAA Cuts at: 2465 2465 Size: 5161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4168 809 Size: 507 2852 1802 Fragments arranged by size: 2852 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2325 2608 Size: 54 1425 50 275 18 312 183 283 Cuts at: 2608 3497 5126 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 312 283 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2608 1537 Size: 275 796 4090 Fragments arranged by size: 4090 796 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2382 3091 1353 Size: 1029 709 3423 Fragments arranged by size: 3423 1029 709 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2070 2105 2270 3748 4122 4268 Size: 1064 138 481 35 165 1478 374 146 Cuts at: 4268 387 Size: 1280 Fragments arranged by size: 1478 1280 1064 481 374 165 146 138 35 EcoICRI GAG'CTC Cuts at: 2392 2392 Size: 5161 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2336 3224 3821 4608 4876 1239 Size: 393 638 66 888 597 787 268 1524 Fragments arranged by size: 1524 888 787 638 597 393 268 66 EcoNI CCTnn'n_nnAGG Cuts at: 2083 2083 Size: 5161 EcoO109I rG'GnC_Cy Cuts at: 2245 2245 Size: 5161 EcoRI G'AATT_C Cuts at: 2470 2848 2470 Size: 378 4783 FspI TGC'GCA Cuts at: 3667 3667 Size: 5161 HaeII r_GCGC'y Cuts at: 1678 2151 2217 4308 1678 Size: 473 66 2091 2531 Fragments arranged by size: 2531 2091 473 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3802 3834 3876 3908 1373 Size: 32 135 32 2230 32 42 32 2626 Fragments arranged by size: 2626 2230 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2070 2505 2751 669 Size: 988 281 132 435 246 3079 Fragments arranged by size: 3079 988 435 281 246 132 HindIII A'AGCT_T Cuts at: 748 748 Size: 5161 HpaI GTT'AAC Cuts at: 2751 2751 Size: 5161 KasI G'GCGC_C Cuts at: 2147 2147 Size: 5161 KpnI G_GTAC'C Cuts at: 2486 2486 Size: 5161 MfeI C'AATT_G Cuts at: 2760 2760 Size: 5161 MluI A'CGCG_T Cuts at: 2373 5037 2373 Size: 2664 2497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4275 4459 1305 Size: 2970 184 2007 Fragments arranged by size: 2970 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3339 Fragments arranged by size: 3339 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2403 3119 3478 3637 518 Size: 648 1237 716 359 159 2042 Fragments arranged by size: 2042 1237 716 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2031 2108 2190 2242 2347 2577 3254 4402 Size: 425 77 82 52 105 230 677 1148 Cuts at: 4402 4647 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 425 245 230 105 82 77 52 NaeI GCC'GGC Cuts at: 2146 2146 Size: 5161 NarI GG'CG_CC Cuts at: 2148 2148 Size: 5161 NcoI C'CATG_G Cuts at: 513 1065 2004 2202 513 Size: 552 939 198 3472 Fragments arranged by size: 3472 939 552 198 NdeI CA'TA_TG Cuts at: 387 387 Size: 5161 NgoMIV G'CCGG_C Cuts at: 2144 2144 Size: 5161 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5161 NotI GC'GGCC_GC Cuts at: 2608 2608 Size: 5161 NspI r_CATG'y Cuts at: 1132 1229 2264 2519 4064 1132 Size: 97 1035 255 1545 2229 Fragments arranged by size: 2229 1545 1035 255 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5161 PciI A'CATG_T Cuts at: 4060 4060 Size: 5161 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5161 PmeI GTTT'AAAC Cuts at: 2465 2465 Size: 5161 PmlI CAC'GTG Cuts at: 2447 2447 Size: 5161 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3230 3262 4743 4775 3230 Size: 32 1481 32 3616 Fragments arranged by size: 3616 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2245 Size: 5161 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5161 PsiI TTA'TAA Cuts at: 2731 2731 Size: 5161 PstI C_TGCA'G Cuts at: 830 2241 2513 830 Size: 1411 272 3478 Fragments arranged by size: 3478 1411 272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5161 PvuII CAG'CTG Cuts at: 2190 2190 Size: 5161 SacI G_AGCT'C Cuts at: 2394 2394 Size: 5161 SalI G'TCGA_C Cuts at: 1936 2503 1936 Size: 567 4594 SapI GCTCTTCn'nnn_ Cuts at: 2382 2382 Size: 5161 SbfI CC_TGCA'GG Cuts at: 2513 2513 Size: 5161 ScaI AGT'ACT Cuts at: 2062 3409 5121 2062 Size: 1347 1712 2102 Fragments arranged by size: 2102 1712 1347 SfcI C'TryA_G Cuts at: 826 1045 2237 2299 2509 3644 4325 4516 826 Size: 219 1192 62 210 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 219 210 191 62 SfoI GGC'GCC Cuts at: 2149 2149 Size: 5161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5161 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5161 SmaI CCC'GGG Cuts at: 1872 2488 1872 Size: 616 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2048 3265 4166 4428 820 Size: 197 926 105 1217 901 262 1553 Fragments arranged by size: 1553 1217 926 901 262 197 105 SnaBI TAC'GTA Cuts at: 493 2952 493 Size: 2459 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5161 SphI G_CATG'C Cuts at: 1132 2519 1132 Size: 1387 3774 SspI AAT'ATT Cuts at: 5 52 3085 5 Size: 47 3033 2081 Fragments arranged by size: 3033 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5161 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 2600 513 Size: 552 939 198 398 3074 Fragments arranged by size: 3074 939 552 398 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3188 3205 3358 3543 3988 325 Size: 1255 1608 17 153 185 445 1498 Fragments arranged by size: 1608 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2060 3407 5119 Size: 274 80 33 51 1443 83 1347 1712 Cuts at: 5119 96 Size: 138 Fragments arranged by size: 1712 1443 1347 274 138 83 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5121 5153 5121 Size: 32 5129 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5161 XbaI T'CTAG_A Cuts at: 2497 2497 Size: 5161 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5161 XmaI C'CCGG_G Cuts at: 1870 2486 1870 Size: 616 4545 XmnI GAAnn'nnTTC Cuts at: 3290 3290 Size: 5161 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2969 276 Size: 53 83 186 936 1435 2468 Fragments arranged by size: 2468 1435 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI