(Circular) (Six-base) MAPSORT of: pFN21AB7430.seq Check: 581 from: 1 to: 6193 pFN21AB7430 6193 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3174 3174 Size: 6193 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 3536 1937 Size: 823 776 4594 Fragments arranged by size: 4594 823 776 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4256 5640 5908 1632 Size: 2624 1384 268 1917 Fragments arranged by size: 2624 1917 1384 268 AfeI AGC'GCT Cuts at: 2329 2329 Size: 6193 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 5092 6069 1203 Size: 3889 977 1327 Fragments arranged by size: 3889 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3363 1184 Size: 2179 4014 AlwNI CAG_nnn'CTG Cuts at: 3936 5508 3936 Size: 1572 4621 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2696 3332 3502 3728 3880 5989 1493 Size: 1203 636 170 226 152 2109 1697 Fragments arranged by size: 2109 1697 1203 636 226 170 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 3518 1870 Size: 73 1575 4545 Fragments arranged by size: 4545 1575 73 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3514 4969 618 Size: 325 217 627 1727 1455 1842 Fragments arranged by size: 1842 1727 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2062 3202 3512 1391 Size: 671 1140 310 4072 Fragments arranged by size: 4072 1140 671 310 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2997 928 Size: 971 1098 4124 Fragments arranged by size: 4124 1098 971 BbvCI CC'TCA_GC Cuts at: 3485 3485 Size: 6193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2517 4804 136 Size: 107 122 71 631 605 845 2287 1525 Fragments arranged by size: 2287 1525 845 631 605 122 107 71 BglII A'GATC_T Cuts at: 6188 6188 Size: 6193 BlpI GC'TnA_GC Cuts at: 3610 3610 Size: 6193 Bme1580I G_kGCm'C Cuts at: 1790 4254 5410 1790 Size: 2464 1156 2573 Fragments arranged by size: 2573 2464 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6193 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3924 4882 442 Size: 416 53 83 399 24 2507 958 1753 Fragments arranged by size: 2507 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2446 2446 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3269 4853 1239 Size: 2030 1584 2579 Fragments arranged by size: 2579 2030 1584 Bpu10I CC'TnA_GC Cuts at: 2571 2636 3485 2571 Size: 65 849 5279 Fragments arranged by size: 5279 849 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4318 5183 5481 4318 Size: 865 298 5030 Fragments arranged by size: 5030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4856 882 Size: 617 3357 2219 Fragments arranged by size: 3357 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3984 493 Size: 711 2780 2702 Fragments arranged by size: 2780 2702 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4000 4382 5017 275 Size: 53 83 186 936 2467 382 635 1451 Fragments arranged by size: 2467 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2026 4626 5024 5298 5445 5856 2026 Size: 2600 398 274 147 411 2363 Fragments arranged by size: 2600 2363 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2161 2191 1178 Size: 30 165 30 758 30 5180 Fragments arranged by size: 5180 758 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6193 BseYI C'CCAG_C Cuts at: 5396 5892 5396 Size: 496 5697 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5620 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2041 3142 3643 4404 5432 1540 Size: 275 186 40 1101 501 761 1028 2301 Fragments arranged by size: 2301 1101 1028 761 501 275 186 40 BsiHKAI G_wGCw'C Cuts at: 4254 4339 5410 4254 Size: 85 1071 5037 Fragments arranged by size: 5037 1071 85 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 3202 3512 4254 4339 5410 1391 Size: 399 272 1140 310 742 85 1071 2174 Fragments arranged by size: 2174 1140 1071 742 399 310 272 85 BspEI T'CCGG_A Cuts at: 2026 5856 2026 Size: 3830 2363 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2113 3013 3174 3548 844 Size: 1269 900 161 374 3489 Fragments arranged by size: 3489 1269 900 374 161 BsrBI CCG'CTC Cuts at: 1394 1823 4080 1394 Size: 429 2257 3507 Fragments arranged by size: 3507 2257 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 4837 5024 1700 Size: 110 265 2762 187 2869 Fragments arranged by size: 2869 2762 265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 2526 794 Size: 1732 4461 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstYI r'GATC_y Cuts at: 1972 3523 3890 4275 4292 5744 5830 5842 6188 Size: 1551 367 385 17 1452 86 12 346 Cuts at: 6188 1972 Size: 1977 Fragments arranged by size: 1977 1551 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5641 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 2490 3709 4503 4523 2490 Size: 1219 794 20 4160 Fragments arranged by size: 4160 1219 794 20 ClaI AT'CG_AT Cuts at: 1554 3138 1554 Size: 1584 4609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2456 2509 Size: 54 1425 50 275 18 208 418 53 Cuts at: 2509 3640 4529 6158 8 Size: 1131 889 1629 43 Fragments arranged by size: 1629 1425 1131 889 418 275 208 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2038 3640 1537 Size: 275 226 1602 4090 Fragments arranged by size: 4090 1602 275 226 EarI CTCTTCn'nnn_ Cuts at: 1353 2298 2437 2529 2737 3411 4123 1353 Size: 945 139 92 208 674 712 3423 Fragments arranged by size: 3423 945 712 674 208 139 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2070 4780 5154 5300 387 Size: 1064 138 481 2710 374 146 1280 Fragments arranged by size: 2710 1280 1064 481 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3269 4256 4853 5640 5908 1239 Size: 393 1637 987 597 787 268 1524 Fragments arranged by size: 1637 1524 987 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2931 2931 Size: 6193 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2737 3128 2737 Size: 391 5802 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 2017 2331 5340 1678 Size: 339 314 3009 2531 Fragments arranged by size: 3009 2531 339 314 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 4834 4866 4908 Size: 32 135 32 503 32 2727 32 42 Cuts at: 4908 4940 1373 Size: 32 2626 Fragments arranged by size: 2727 2626 503 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2849 3473 3537 3783 669 Size: 988 281 911 624 64 246 3079 Fragments arranged by size: 3079 988 911 624 281 246 64 HindIII A'AGCT_T Cuts at: 748 3344 748 Size: 2596 3597 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 2598 3792 2598 Size: 1194 4999 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5307 5491 1305 Size: 4002 184 2007 Fragments arranged by size: 4002 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2511 10 Size: 54 1425 343 679 3692 Fragments arranged by size: 3692 1425 679 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2095 3168 4151 4510 4669 518 Size: 648 929 1073 983 359 159 2042 Fragments arranged by size: 2042 1073 983 929 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2914 3609 4286 5434 5679 1606 Size: 431 877 695 677 1148 245 2120 Fragments arranged by size: 2120 1148 877 695 677 431 245 NaeI GCC'GGC Cuts at: 2077 2077 Size: 6193 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5641 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NgoMIV G'CCGG_C Cuts at: 2075 2075 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 3209 3551 5096 1132 Size: 97 1980 342 1545 2229 Fragments arranged by size: 2229 1980 1545 342 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6193 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6193 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2850 2882 2850 Size: 32 6161 PstI C_TGCA'G Cuts at: 830 3545 830 Size: 2715 3478 PvuI CG_AT'CG Cuts at: 2001 3142 2001 Size: 1141 5052 PvuII CAG'CTG Cuts at: 2914 2914 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2707 2757 3541 4676 5357 5548 826 Size: 219 1662 50 784 1135 681 191 1471 Fragments arranged by size: 1662 1471 1135 784 681 219 191 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4297 5198 5460 820 Size: 197 926 2354 901 262 1553 Fragments arranged by size: 2354 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 2834 4117 5 Size: 47 2782 1283 2081 Fragments arranged by size: 2782 2081 1283 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2969 3632 513 Size: 552 1904 663 3074 Fragments arranged by size: 3074 1904 663 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4220 4237 4390 4575 5020 325 Size: 1255 2640 17 153 185 445 1498 Fragments arranged by size: 2640 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2381 4439 6151 Size: 274 80 33 51 1443 404 2058 1712 Cuts at: 6151 96 Size: 138 Fragments arranged by size: 2058 1712 1443 404 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2122 2154 6153 6185 2122 Size: 32 3999 32 2130 Fragments arranged by size: 3999 2130 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2466 2466 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 2450 2994 3111 4322 2450 Size: 544 117 1211 4321 Fragments arranged by size: 4321 1211 544 117 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI