Restriction map for
pFN21AB7429
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I ClaI CpoI DpnII DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KpnI MboI MfeI
MflI MluI MroI MunI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tsp509I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 586 GTAC GT^AC
AlwNI 191 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 770 AGCGCT AGC^GCT
AspHI 207 GAGCAC G[AT]GC[AT]^C
AspI 880 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 641 TGGCCA TGG^CCA
BciVI 865 GGATAC GTATCC(6/5)
BlpI 105 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 105 GCTTAGC GC^T[ACGT]AGC
BsaBI 507 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 706 GAGACC GGTCTC(1/5)
BseRI 138 GAGGAG GAGGAG(10/8)
BsiHKAI 207 GAGCAC G[AT]GC[AT]^C
BssHII 635 GCGCGC G^CGCGC
BstAPI 875 GCACCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 105 GCTTAGC GC^T[ACGT]AGC
Eco47III 770 AGCGCT AGC^GCT
EcoNI 152 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 718 GATTC G^A[ACGT]TC
KspI 212 CCGCGG CCGC^GG
MaeIII 310 GTGAC ^GT[ACGT]AC
MamI 507 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 641 TGGCCA TGG^CCA
MscI 641 TGGCCA TGG^CCA
MseI 554 TTAA T^TAA
MspA1I 212 CCGCGG C[AC]G^C[GT]G
PflFI 880 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 801 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 482 GGGACCT [GA]G^G[AT]CC[CT]
PstI 70 CTGCAG CTGCA^G
RsaI 586 GTAC GT^AC
SacII 212 CCGCGG CCGC^GG
SfcI 70 CTGCAG C^T[GA][CT]AG
SmlI 649 CTCAAG C^T[CT][GA]AG
StuI 149 AGGCCT AGG^CCT
TfiI 718 GATTC G^A[AT]TC
Tru9I 554 TTAA T^TAA
Tsp45I 310 GTGAC ^GT[GC]AC
Tth111I 880 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 801 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 455 CCACACGCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 54 CCTCAGG CC^T[ACGT]AGG
689 CCTCAGG
ApaI 591 GGGCCC GGGCC^C
621 GGGCCC
AvaII 468 GGACC G^G[AT]CC
483 GGACC
BanII 591 GGGCCC G[GA]GC[CT]^C
621 GGGCCC
BfaI 172 CTAG C^TAG
296 CTAG
BpmI 229 CTGGAG CTGGAG(16/14)
244 CTGGAG
BsgI 627 CTGCAC GTGCAG(16/14)
544 GTGCAG
BsmAI 706 GAGAC GTCTC(1/5)
753 GTCTC
BsmFI 482 GGGAC GGGAC(10/14)
698 GGGAC
BsrI 588 ACTGG ACTGG(1/-1)
495 CCAGT
BssSI 274 CTCGTG CACGAG(-5/-1)
670 CTCGTG
Bsu36I 54 CCTCAGG CC^T[ACGT]AGG
689 CCTCAGG
DraIII 492 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
802 CACTTCGTG
DsaI 212 CCGCGG C^C[GA][CT]GG
302 CCGTGG
EarI 40 GAAGAG CTCTTC(1/4)
95 GAAGAG
Eco81I 54 CCTCAGG CC^T[ACGT]AGG
689 CCTCAGG
EcoT14I 62 CCTTGG C^C[AT][AT]GG
314 CCAAGG
HgaI 700 GACGC GACGC(5/10)
884 GCGTC
Ksp632I 40 GAAGAG CTCTTC(1/4)
95 GAAGAG
MaeI 172 CTAG C^TAG
296 CTAG
MaeII 743 ACGT A^CGT
833 ACGT
MslI 292 CATGCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
537 CACTGAGGTG
NaeI 13 GCCGGC GCC^GGC
811 GCCGGC
NgoMIV 13 GCCGGC G^CCGGC
811 GCCGGC
SapI 40 GAAGAGC GCTCTTC(1/4)
95 GAAGAGC
SfaNI 580 GATGC GCATC(5/9)
477 GCATC
StyI 62 CCTTGG C^C[AT][AT]GG
314 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbeI BbvI BcnI BmyI BsaHI
BsaJI BsiYI BslI Bsp1286I BspMI
BsrBI BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI KasI MboII MnlI MspI
MvaI MvnI MwoI NarI NciI
NlaIII NlaIV Sau96I ScrFI SfoI
TseI TspRI