Restriction map for
pFN21AB7429
(Linear) (Six-base) MAPSORT of: KIBB7429.seq Check: 2954 from: 1 to: 897
KIBB7429.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 425 897
Size: 425 472
AfeI AGC'GCT
Cuts at: 0 772 897
Size: 772 125
AleI CACnn'nnGTG
Cuts at: 0 541 897
Size: 541 356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 838 897
Size: 838 59
AlwNI CAG_nnn'CTG
Cuts at: 0 196 897
Size: 196 701
ApaI G_GGCC'C
Cuts at: 0 595 625 897
Size: 595 30 272
Fragments arranged by size:
595 272 30
BanI G'GyrC_C
Cuts at: 0 33 413 762 794 874 897
Size: 33 380 349 32 80 23
Fragments arranged by size:
380 349 80 33 32 23
BanII G_rGCy'C
Cuts at: 0 595 625 897
Size: 595 30 272
Fragments arranged by size:
595 272 30
BbeI G_GCGC'C
Cuts at: 0 37 766 798 897
Size: 37 729 32 99
Fragments arranged by size:
729 99 37 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 859 897
Size: 859 38
BlpI GC'TnA_GC
Cuts at: 0 106 897
Size: 106 791
Bme1580I G_kGCm'C
Cuts at: 0 411 595 625 827 877 897
Size: 411 184 30 202 50 20
Fragments arranged by size:
411 202 184 50 30 20
BmgBI CAC'GTC
Cuts at: 0 744 834 897
Size: 744 90 63
Fragments arranged by size:
744 90 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 489 598 897
Size: 489 109 299
Fragments arranged by size:
489 299 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 897
Size: 126 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 265 897
Size: 250 15 632
Fragments arranged by size:
632 250 15
Bpu10I CC'TnA_GC
Cuts at: 0 523 897
Size: 523 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 897
Size: 634 263
BsaI GGTCTCn'nnnn_
Cuts at: 0 700 897
Size: 700 197
BsaBI GATnn'nnATC
Cuts at: 0 511 897
Size: 511 386
BsaHI Gr'CG_yC
Cuts at: 0 34 763 795 897
Size: 34 729 32 102
Fragments arranged by size:
729 102 34 32
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 153 897
Size: 153 744
BseYI C'CCAG_C
Cuts at: 0 695 897
Size: 695 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 612 897
Size: 565 47 285
Fragments arranged by size:
565 285 47
BsiHKAI G_wGCw'C
Cuts at: 0 211 897
Size: 211 686
Bsp1286I G_dGCh'C
Cuts at: 0 211 411 595 625 827 877 897
Size: 211 200 184 30 202 50 20
Fragments arranged by size:
211 202 200 184 50 30 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 425 479 539 806 897
Size: 425 54 60 267 91
Fragments arranged by size:
425 267 91 60 54
BsrBI CCG'CTC
Cuts at: 0 45 100 234 334 897
Size: 45 55 134 100 563
Fragments arranged by size:
563 134 100 55 45
BsrFI r'CCGG_y
Cuts at: 0 13 709 811 897
Size: 13 696 102 86
Fragments arranged by size:
696 102 86 13
BssHII G'CGCG_C
Cuts at: 0 635 897
Size: 635 262
BssSI C'ACGA_G
Cuts at: 0 274 670 897
Size: 274 396 227
Fragments arranged by size:
396 274 227
BstAPI GCAn_nnn'nTGC
Cuts at: 0 881 897
Size: 881 16
Bsu36I CC'TnA_GG
Cuts at: 0 55 690 897
Size: 55 635 207
Fragments arranged by size:
635 207 55
BtgI C'CryG_G
Cuts at: 0 212 302 897
Size: 212 90 595
Fragments arranged by size:
595 212 90
BtsI GCAGTG_nn'
Cuts at: 0 398 897
Size: 398 499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 111 146 897
Size: 111 35 751
Fragments arranged by size:
751 111 35
DraIII CAC_nnn'GTG
Cuts at: 0 497 807 897
Size: 497 310 90
Fragments arranged by size:
497 310 90
EaeI y'GGCC_r
Cuts at: 0 641 780 809 897
Size: 641 139 29 88
Fragments arranged by size:
641 139 88 29
EarI CTCTTCn'nnn_
Cuts at: 0 35 90 897
Size: 35 55 807
Fragments arranged by size:
807 55 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 265 897
Size: 250 15 632
Fragments arranged by size:
632 250 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 156 897
Size: 156 741
EcoO109I rG'GnC_Cy
Cuts at: 0 59 193 483 592 621 622 897
Size: 59 134 290 109 29 1 275
Fragments arranged by size:
290 275 134 109 59 29 1
HaeII r_GCGC'y
Cuts at: 0 37 766 774 798 897
Size: 37 729 8 24 99
Fragments arranged by size:
729 99 37 24 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 897
Size: 673 32 192
Fragments arranged by size:
673 192 32
KasI G'GCGC_C
Cuts at: 0 33 762 794 897
Size: 33 729 32 103
Fragments arranged by size:
729 103 33 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 897
Size: 8 889
MscI TGG'CCA
Cuts at: 0 643 897
Size: 643 254
MslI CAynn'nnrTG
Cuts at: 0 296 541 897
Size: 296 245 356
Fragments arranged by size:
356 296 245
MspA1I CmG'CkG
Cuts at: 0 214 897
Size: 214 683
NaeI GCC'GGC
Cuts at: 0 15 813 897
Size: 15 798 84
Fragments arranged by size:
798 84 15
NarI GG'CG_CC
Cuts at: 0 34 763 795 897
Size: 34 729 32 102
Fragments arranged by size:
729 102 34 32
NgoMIV G'CCGG_C
Cuts at: 0 13 811 897
Size: 13 798 86
Fragments arranged by size:
798 86 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 807 897
Size: 807 90
PfoI T'CCnGG_A
Cuts at: 0 340 897
Size: 340 557
PpuMI rG'GwC_Cy
Cuts at: 0 483 897
Size: 483 414
PspOMI G'GGCC_C
Cuts at: 0 591 621 897
Size: 591 30 276
Fragments arranged by size:
591 276 30
PstI C_TGCA'G
Cuts at: 0 74 897
Size: 74 823
SacII CC_GC'GG
Cuts at: 0 215 897
Size: 215 682
SapI GCTCTTCn'nnn_
Cuts at: 0 35 90 897
Size: 35 55 807
Fragments arranged by size:
807 55 35
SfcI C'TryA_G
Cuts at: 0 70 897
Size: 70 827
SfoI GGC'GCC
Cuts at: 0 35 764 796 897
Size: 35 729 32 101
Fragments arranged by size:
729 101 35 32
SmlI C'TyrA_G
Cuts at: 0 649 897
Size: 649 248
StuI AGG'CCT
Cuts at: 0 151 897
Size: 151 746
StyI C'CwwG_G
Cuts at: 0 62 314 897
Size: 62 252 583
Fragments arranged by size:
583 252 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 508 897
Size: 508 389
Tth111I GACn'n_nGTC
Cuts at: 0 883 897
Size: 883 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 462 897
Size: 462 435
Enzymes that do cut:
AarI AfeI AleI AlfI AlwNI ApaI BanI BanII
BbeI BciVI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI
Bsu36I BtgI BtsI CspCI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV PflMI PfoI PpuMI PspOMI
PstI SacII SapI SfcI SfoI SmlI StuI StyI
TaqII Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BmtI BsaAI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII
BstXI BstYI BstZ17I BtgZI ClaI DraI DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI XhoI XmaI XmnI
ZraI