(Circular) (Six-base) MAPSORT of: pFN21AB7426.seq Check: 6319 from: 1 to: 5941 pFN21AB7426 5941 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4004 5388 5656 1632 Size: 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 268 AfeI AGC'GCT Cuts at: 2411 3220 2411 Size: 809 5132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 2016 3684 5256 2016 Size: 1668 1572 2701 Fragments arranged by size: 2701 1668 1572 ApaI G_GGCC'C Cuts at: 2670 2670 Size: 5941 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2270 2569 2669 2863 3266 1870 Size: 73 93 234 299 100 194 403 4545 Fragments arranged by size: 4545 403 299 234 194 100 93 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2119 2460 2582 2641 3262 Size: 325 217 627 332 341 122 59 621 Cuts at: 3262 4717 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 621 341 332 325 217 122 59 BanII G_rGCy'C Cuts at: 1391 2670 2720 2811 3260 1391 Size: 1279 50 91 449 4072 Fragments arranged by size: 4072 1279 449 91 50 BbeI G_GCGC'C Cuts at: 2464 2645 2464 Size: 181 5760 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3236 928 Size: 971 1337 3633 Fragments arranged by size: 3633 1337 971 BbvCI CC'TCA_GC Cuts at: 2792 2792 Size: 5941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2059 2580 2629 Size: 107 122 71 631 605 387 521 49 Cuts at: 2629 2665 4552 136 Size: 36 1887 1525 Fragments arranged by size: 1887 1525 631 605 521 387 122 107 71 49 36 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 2399 3358 2399 Size: 959 4982 Bme1580I G_kGCm'C Cuts at: 1790 2670 4002 5158 1790 Size: 880 1332 1156 2573 Fragments arranged by size: 2573 1332 1156 880 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3207 4601 1239 Size: 1968 1394 2579 Fragments arranged by size: 2579 1968 1394 Bpu10I CC'TnA_GC Cuts at: 2792 2792 Size: 5941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2666 4604 882 Size: 617 1167 1938 2219 Fragments arranged by size: 2219 1938 1167 617 BsaAI yAC'GTr Cuts at: 493 1204 2353 3732 493 Size: 711 1149 1379 2702 Fragments arranged by size: 2702 1379 1149 711 BsaBI GATnn'nnATC Cuts at: 2189 5935 2189 Size: 3746 2195 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2461 2538 2642 3748 Size: 53 83 186 936 928 77 104 1106 Cuts at: 3748 4130 4765 275 Size: 382 635 1451 Fragments arranged by size: 1451 1106 936 928 635 382 186 104 83 77 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 3183 3213 1178 Size: 30 165 30 639 30 1111 30 3906 Fragments arranged by size: 3906 1111 639 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2590 3204 1186 Size: 1404 614 3923 Fragments arranged by size: 3923 1404 614 BseYI C'CCAG_C Cuts at: 2728 5144 5640 2728 Size: 2416 496 3029 Fragments arranged by size: 3029 2416 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2350 2369 1163 Size: 573 614 19 4735 Fragments arranged by size: 4735 614 573 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2714 2843 2859 3391 4152 5180 Size: 275 186 713 129 16 532 761 1028 Cuts at: 5180 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 713 532 275 186 129 16 BsiHKAI G_wGCw'C Cuts at: 2490 4002 4087 5158 2490 Size: 1512 85 1071 3273 Fragments arranged by size: 3273 1512 1071 85 BsmI GAATG_Cn' Cuts at: 2711 3452 3545 2711 Size: 741 93 5107 Fragments arranged by size: 5107 741 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2490 2670 2720 2811 3260 4002 4087 Size: 399 700 180 50 91 449 742 85 Cuts at: 4087 5158 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 700 449 399 180 91 85 50 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2166 2434 3095 3828 1394 Size: 429 343 268 661 733 3507 Fragments arranged by size: 3507 733 661 429 343 268 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2122 2513 2709 2835 2883 4585 4772 Size: 110 312 391 196 126 48 1702 187 Cuts at: 4772 1700 Size: 2869 Fragments arranged by size: 2869 1702 391 312 196 187 126 110 48 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 2472 2733 1925 Size: 547 261 5133 Fragments arranged by size: 5133 547 261 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 2125 2176 2452 2755 1184 Size: 941 51 276 303 4370 Fragments arranged by size: 4370 941 303 276 51 BstYI r'GATC_y Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936 Size: 1299 367 385 17 1452 86 12 346 Cuts at: 5936 1972 Size: 1977 Fragments arranged by size: 1977 1452 1299 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2057 2496 2676 2685 513 Size: 552 939 53 439 180 9 3769 Fragments arranged by size: 3769 939 552 439 180 53 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2464 487 Size: 683 56 1238 3964 Fragments arranged by size: 3964 1238 683 56 BtsI GCAGTG_nn' Cuts at: 3159 3457 4251 4271 3159 Size: 298 794 20 4829 Fragments arranged by size: 4829 794 298 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3106 3245 3106 Size: 139 5802 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2244 2418 2711 Size: 54 1425 50 275 18 414 174 293 Cuts at: 2711 2840 2856 3388 4277 5906 8 Size: 129 16 532 889 1629 43 Fragments arranged by size: 1629 1425 889 532 414 293 275 174 129 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2711 2840 2856 3388 1537 Size: 275 899 129 16 532 4090 Fragments arranged by size: 4090 899 532 275 129 16 EarI CTCTTCn'nnn_ Cuts at: 1353 3871 1353 Size: 2518 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3207 4004 4601 5388 5656 1239 Size: 393 1575 797 597 787 268 1524 Fragments arranged by size: 1575 1524 797 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2548 2666 2548 Size: 118 5823 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2089 2117 2200 2305 2413 2464 2504 2645 Size: 411 28 83 105 108 51 40 141 Cuts at: 2645 2693 3222 5088 1678 Size: 48 529 1866 2531 Fragments arranged by size: 2531 1866 529 411 141 108 105 83 51 48 40 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373 Size: 32 135 32 3010 32 42 32 2626 Fragments arranged by size: 3010 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2460 2641 2460 Size: 181 5760 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 5055 5239 1305 Size: 972 2778 184 2007 Fragments arranged by size: 2778 2007 972 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2420 10 Size: 54 1425 343 588 3531 Fragments arranged by size: 3531 1425 588 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2483 3899 4258 4417 518 Size: 648 1080 237 1416 359 159 2042 Fragments arranged by size: 2042 1416 1080 648 359 237 159 MspA1I CmG'CkG Cuts at: 1606 2091 2132 2329 2678 2687 2728 2845 3357 Size: 485 41 197 349 9 41 117 512 Cuts at: 3357 4034 5182 5427 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 512 485 349 245 197 117 41 41 9 NaeI GCC'GGC Cuts at: 2711 2837 2711 Size: 126 5815 NarI GG'CG_CC Cuts at: 2461 2642 2461 Size: 181 5760 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 2709 2835 2709 Size: 126 5815 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 2840 3388 2840 Size: 548 5393 NspI r_CATG'y Cuts at: 1132 1229 2212 3299 4844 1132 Size: 97 983 1087 1545 2229 Fragments arranged by size: 2229 1545 1087 983 97 PasI CC'CwG_GG Cuts at: 1348 2107 2313 1348 Size: 759 206 4976 Fragments arranged by size: 4976 759 206 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 2548 Size: 5941 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 2927 3511 2927 Size: 584 5357 PspOMI G'GGCC_C Cuts at: 2666 2666 Size: 5941 PstI C_TGCA'G Cuts at: 830 3293 830 Size: 2463 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2728 2728 Size: 5941 SacII CC_GC'GG Cuts at: 2679 2688 2679 Size: 9 5932 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 3008 4189 5901 3008 Size: 1181 1712 3048 Fragments arranged by size: 3048 1712 1181 SfcI C'TryA_G Cuts at: 826 1045 3289 4424 5105 5296 826 Size: 219 2244 1135 681 191 1471 Fragments arranged by size: 2244 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2462 2643 2462 Size: 181 5760 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2571 3268 1872 Size: 699 697 4545 Fragments arranged by size: 4545 699 697 SmlI C'TyrA_G Cuts at: 820 1017 1943 4045 4946 5208 820 Size: 197 926 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 3380 513 Size: 552 939 1376 3074 Fragments arranged by size: 3074 1376 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2305 3968 3985 4138 4323 4768 325 Size: 1255 725 1663 17 153 185 445 1498 Fragments arranged by size: 1663 1498 1255 725 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2994 3006 3087 Size: 274 80 33 51 1443 1017 12 81 Cuts at: 3087 4187 5899 96 Size: 1100 1712 138 Fragments arranged by size: 1712 1443 1100 1017 274 138 81 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 3183 3215 5901 5933 2042 Size: 32 1109 32 2686 32 2050 Fragments arranged by size: 2686 2050 1109 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2230 2230 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2569 3266 1870 Size: 699 697 4545 Fragments arranged by size: 4545 699 697 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI