Restriction map for
pFN21AB7423
##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AfaI 354 GTAC GT^AC
AflIII 31 ACATGT A^C[GA][CT]GT
AlwNI 73 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 157 GGTCC G^G[AT]CC
BanI 198 GGTGCC G^G[CT][GA]CC
BbsI 569 GAAGAC GAAGAC(2/6)
BclI 183 TGATCA T^GATCA
BcnI 413 CCGGG CC^[GC]GG
BpuAI 569 GAAGAC GAAGAC(2/6)
BsaAI 442 CACGTA [CT]AC^GT[GA]
BsgI 546 GTGCAG GTGCAG(16/14)
BsmBI 56 CGTCTC CGTCTC(1/5)
BspLU11I 31 ACATGT A^CATGT
BsrDI 660 CATTGC GCAATG(2/0)
BstAPI 368 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 500 GGATCT [GA]^GATC[CT]
CfoI 13 GCGC GCG^C
Cfr13I 157 GGTCC G^G[ACGT]CC
EarI 666 CTCTTC CTCTTC(1/4)
FauI 497 GCGGG CCCGC(4/6)
FbaI 183 TGATCA T^GATCA
HaeII 12 GGCGCT [GA]GCGC^[CT]
HaeIII 531 GGCC GG^CC
HapII 413 CCGG C^CGG
HgaI 55 GCGTC GACGC(5/10)
HhaI 13 GCGC GCG^C
HinP1I 13 GCGC G^CGC
HincII 232 GTCAAC GT[CT]^[GA]AC
HindII 232 GTCAAC GT[CT]^[GA]AC
HpaII 413 CCGG C^CGG
Ksp632I 666 CTCTTC CTCTTC(1/4)
MaeII 443 ACGT A^CGT
MaeIII 284 GTTAC ^GT[ACGT]AC
MflI 500 GGATCT [GA]^GATC[CT]
MseI 95 TTAA T^TAA
MspA1I 76 CCGCTG C[AC]G^C[GT]G
MspI 413 CCGG C^CGG
NciI 413 CCGGG CC^[GC]GG
PleI 587 GACTC GAGTC(4/5)
RsaI 354 GTAC GT^AC
Sau96I 157 GGTCC G^G[ACGT]CC
SfcI 291 CTGTAG C^T[GA][CT]AG
TaqI 251 TCGA T^CGA
Tru9I 95 TTAA T^TAA
Tsp509I 8 AATT ^AATT
TthHB8I 251 TCGA T^CGA
XhoII 500 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 439 GTGCAC G^TGCAC
579 GTGCAC
AlwI 500 GGATC GGATC(4/5)
724 GGATC
ApaLI 439 GTGCAC G^TGCAC
579 GTGCAC
BsiYI 172 CCTCTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
671 CCTGCCCTGGG
BslI 172 CCTCTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
671 CCTGCCCTGGG
BstF5I 3 GGATG GGATG(2/0)
123 GGATG
Cac8I 71 GCCAGC GC[ACGT]^[ACGT]GC
532 GCCAGC
DdeI 44 CTGAG C^T[ACGT]AG
349 CTAAG
EcoT14I 177 CCTTGG C^C[AT][AT]GG
452 CCAAGG
FokI 3 GGATG GGATG(9/13)
123 GGATG
HphI 181 GGTGA GGTGA(8/7)
113 TCACC
MslI 83 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
432 CACGGGAGTG
MwoI 368 GCAAGGTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
459 GCAGGAGGAGC
NspI 31 ACATGT [GA]CATG^[CT]
391 GCATGT
StyI 177 CCTTGG C^C[AT][AT]GG
452 CCAAGG
TfiI 216 GAATC G^A[AT]TC
253 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
DpnII EcoRII Fnu4HI HinfI ItaI
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV Sau3AI ScrFI TseI
TspRI