Restriction map for
pFN21AB7421
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MroI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TthHB8I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 481 GAATTC [GA]^AATT[CT]
AflIII 72 ACATGT A^C[GA][CT]GT
Alw44I 438 GTGCAC G^TGCAC
AocI 218 CCTCAGG CC^T[ACGT]AGG
ApaLI 438 GTGCAC G^TGCAC
ApoI 481 GAATTC [GA]^AATT[CT]
AseI 64 ATTAAT AT^TAAT
AsnI 64 ATTAAT AT^TAAT
AspI 961 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 662 CCCGGG C^[CT]CG[GA]G
BanI 690 GGCACC G^G[CT][GA]CC
BbvCI 336 CCTCAGC CCTCAGC(-5/-2)
BpmI 1043 CTGGAG CTGGAG(16/14)
BseRI 334 CTCCTC GAGGAG(10/8)
BsgI 716 GTGCAG GTGCAG(16/14)
BsmAI 736 GAGAC GTCTC(1/5)
BsmI 97 GAATGC GAATGC(1/-1)
BsoBI 662 CCCGGG C^[CT]CG[GA]G
Bsp1407I 917 TGTACA T^GTACA
BspHI 554 TCATGA T^CATGA
BspLU11I 72 ACATGT A^CATGT
BsrGI 917 TGTACA T^GTACA
BsrI 1042 ACTGG ACTGG(1/-1)
Bsu36I 218 CCTCAGG CC^T[ACGT]AGG
Cac8I 951 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 309 GCGC GCG^C
DrdI 885 GACTGTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 218 CCTCAGG CC^T[ACGT]AGG
EcoNI 1061 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 481 GAATTC G^AATTC
EcoT14I 1061 CCTTGG C^C[AT][AT]GG
HhaI 309 GCGC GCG^C
HinP1I 309 GCGC G^CGC
MaeIII 1039 GTGAC ^GT[ACGT]AC
PflFI 961 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1077 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 64 ATTAAT AT^TAAT
RcaI 554 TCATGA T^CATGA
SmaI 662 CCCGGG CCC^GGG
SspBI 917 TGTACA T^GTACA
StyI 1061 CCTTGG C^C[AT][AT]GG
Tsp45I 1039 GTGAC ^GT[GC]AC
Tth111I 961 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 422 CCATTTCCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 662 CCCGGG C^CCGGG
XmaI 662 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 419 GGACC G^G[AT]CC
1078 GGTCC
BalI 269 TGGCCA TGG^CCA
521 TGGCCA
BspMI 404 ACCTGC ACCTGC(4/8)
586 GCAGGT
DraI 21 TTTAAA TTT^AAA
42 TTTAAA
DraII 1057 AGGGCCT [GA]G^G[ACGT]CC[CT]
1077 GGGTCCT
EaeI 269 TGGCCA [CT]^GGCC[GA]
521 TGGCCA
EarI 157 GAAGAG CTCTTC(1/4)
1053 GAAGAG
EcoO109I 1057 AGGGCCT [GA]G^G[ACGT]CC[CT]
1077 GGGTCCT
HapII 564 CCGG C^CGG
663 CCGG
HinfI 774 GATTC G^A[ACGT]TC
809 GAATC
HpaII 564 CCGG C^CGG
663 CCGG
HphI 257 TCACC GGTGA(8/7)
635 TCACC
Ksp632I 157 GAAGAG CTCTTC(1/4)
1053 GAAGAG
MluNI 269 TGGCCA TGG^CCA
521 TGGCCA
MscI 269 TGGCCA TGG^CCA
521 TGGCCA
MspI 564 CCGG C^CGG
663 CCGG
SacI 834 GAGCTC GAGCT^C
1046 GAGCTC
SfaNI 390 GATGC GCATC(5/9)
765 GATGC
TfiI 774 GATTC G^A[AT]TC
809 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII ItaI
MboI MboII MnlI MseI MslI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfcI Tru9I TseI Tsp509I
TspRI