(Circular) (Six-base) MAPSORT of: pFN21AB7421.seq Check: 1873 from: 1 to: 5812 pFN21AB7421 5812 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 1937 Size: 1218 4594 Acc65I G'GTAC_C Cuts at: 3133 3133 Size: 5812 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2729 3077 3875 5259 5527 1632 Size: 1097 348 798 1384 268 1917 Fragments arranged by size: 1917 1384 1097 798 348 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 2077 4711 5688 1203 Size: 874 2634 977 1327 Fragments arranged by size: 2634 1327 977 874 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 2384 1166 Size: 1218 4594 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 3555 5127 3555 Size: 1572 4240 ApaLI G'TGCA_C Cuts at: 2443 3869 5025 2443 Size: 1426 1156 3230 Fragments arranged by size: 3230 1426 1156 ApoI r'AATT_y Cuts at: 1493 2486 3121 3347 3499 5608 1493 Size: 993 635 226 152 2109 1697 Fragments arranged by size: 2109 1697 993 635 226 152 AseI AT'TA_AT Cuts at: 160 1032 2070 4366 160 Size: 872 1038 2296 1606 Fragments arranged by size: 2296 1606 1038 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 2667 3137 1870 Size: 73 724 470 4545 Fragments arranged by size: 4545 724 470 73 BamHI G'GATC_C Cuts at: 3142 3509 3142 Size: 367 5445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2695 3133 4588 618 Size: 325 217 627 908 438 1455 1842 Fragments arranged by size: 1842 1455 908 627 438 325 217 BanII G_rGCy'C Cuts at: 1391 2612 2843 3055 3131 1391 Size: 1221 231 212 76 4072 Fragments arranged by size: 4072 1221 231 212 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4841 BbvCI CC'TCA_GC Cuts at: 2342 2342 Size: 5812 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 3229 3229 Size: 5812 Bme1580I G_kGCm'C Cuts at: 1790 2447 3873 5029 1790 Size: 657 1426 1156 2573 Fragments arranged by size: 2573 1426 1156 657 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3543 4501 442 Size: 416 53 83 399 24 2126 958 1753 Fragments arranged by size: 2126 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3069 4472 1239 Size: 1830 1403 2579 Fragments arranged by size: 2579 1830 1403 Bpu10I CC'TnA_GC Cuts at: 2342 2342 Size: 5812 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4475 882 Size: 617 2976 2219 Fragments arranged by size: 2976 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3603 493 Size: 711 2399 2702 Fragments arranged by size: 2702 2399 711 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3619 4001 4636 275 Size: 53 83 186 936 2086 382 635 1451 Fragments arranged by size: 2086 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4245 4643 4917 5064 5475 4245 Size: 398 274 147 411 4582 Fragments arranged by size: 4582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5587 Fragments arranged by size: 5587 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 1186 Size: 1144 4668 BseYI C'CCAG_C Cuts at: 2497 5015 5511 2497 Size: 2518 496 2798 Fragments arranged by size: 2798 2518 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2742 1163 Size: 573 1006 4233 Fragments arranged by size: 4233 1006 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2447 2843 3055 3873 3958 5029 2447 Size: 396 212 818 85 1071 3230 Fragments arranged by size: 3230 1071 818 396 212 85 BsmI GAATG_Cn' Cuts at: 2108 3323 3416 2108 Size: 1215 93 4504 Fragments arranged by size: 4504 1215 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2447 2612 2843 3055 3131 3873 3958 Size: 399 657 165 231 212 76 742 85 Cuts at: 3958 5029 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 657 399 231 212 165 85 76 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 2559 3701 5431 2559 Size: 1142 1730 2940 Fragments arranged by size: 2940 1730 1142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2418 2582 3167 844 Size: 1574 164 585 3489 Fragments arranged by size: 3489 1574 585 164 BsrBI CCG'CTC Cuts at: 1394 1823 3699 1394 Size: 429 1876 3507 Fragments arranged by size: 3507 1876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4307 4481 65 Size: 1183 3059 174 1396 Fragments arranged by size: 3059 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 2922 96 Size: 2826 2986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5812 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5812 BstYI r'GATC_y Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807 Size: 1170 367 385 17 1452 86 12 346 Cuts at: 5807 1972 Size: 1977 Fragments arranged by size: 1977 1452 1170 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2224 2224 Size: 5812 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5260 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5073 Fragments arranged by size: 5073 683 56 BtsI GCAGTG_nn' Cuts at: 3328 4122 4142 3328 Size: 794 20 4998 Fragments arranged by size: 4998 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 2028 2049 3116 2028 Size: 21 1067 4724 Fragments arranged by size: 4724 1067 21 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2896 4819 809 Size: 507 1580 1923 1802 Fragments arranged by size: 1923 1802 1580 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2274 2526 3259 Size: 54 1425 50 275 18 444 252 733 Cuts at: 3259 4148 5777 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 733 444 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 3053 3742 1353 Size: 804 896 689 3423 Fragments arranged by size: 3423 896 804 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4399 4773 4919 387 Size: 1064 138 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2841 3053 2841 Size: 212 5600 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2729 3069 3077 3875 4472 5259 5527 Size: 393 1097 340 8 798 597 787 268 Cuts at: 5527 1239 Size: 1524 Fragments arranged by size: 1524 1097 798 787 597 393 340 268 8 EcoNI CCTnn'n_nnAGG Cuts at: 3070 3070 Size: 5812 EcoO109I rG'GnC_Cy Cuts at: 3063 3083 3063 Size: 20 5792 EcoRI G'AATT_C Cuts at: 2486 3121 3499 2486 Size: 635 378 4799 Fragments arranged by size: 4799 635 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3082 3082 Size: 5812 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 4959 1678 Size: 3281 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2538 2570 3037 3069 4453 Size: 32 135 32 966 32 467 32 1384 Cuts at: 4453 4485 4527 4559 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1384 966 467 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3156 3402 669 Size: 988 281 1218 246 3079 Fragments arranged by size: 3079 1218 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KpnI G_GTAC'C Cuts at: 3137 3137 Size: 5812 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2558 4926 5110 1305 Size: 1253 2368 184 2007 Fragments arranged by size: 2368 2007 1253 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2276 2528 10 Size: 54 1425 343 444 252 3294 Fragments arranged by size: 3294 1425 444 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 2384 2642 2891 2930 3002 3770 Size: 648 1029 189 258 249 39 72 768 Cuts at: 3770 4129 4288 518 Size: 359 159 2042 Fragments arranged by size: 2042 1029 768 648 359 258 249 189 159 72 39 MspA1I CmG'CkG Cuts at: 1606 3228 3905 5053 5298 1606 Size: 1622 677 1148 245 2120 Fragments arranged by size: 2120 1622 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5260 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2081 2721 2935 3170 4715 1132 Size: 97 852 640 214 235 1545 2229 Fragments arranged by size: 2229 1545 852 640 235 214 97 PasI CC'CwG_GG Cuts at: 1348 2097 1348 Size: 749 5063 PciI A'CATG_T Cuts at: 2077 4711 2077 Size: 2634 3178 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3083 3083 Size: 5812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PstI C_TGCA'G Cuts at: 830 3164 830 Size: 2334 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 SacI G_AGCT'C Cuts at: 2843 3055 2843 Size: 212 5600 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 2148 2181 2790 2823 3022 3160 4295 Size: 219 1103 33 609 33 199 138 1135 Cuts at: 4295 4976 5167 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1103 681 609 219 199 191 138 33 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 2669 3139 1872 Size: 797 470 4545 Fragments arranged by size: 4545 797 470 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 4817 5079 820 Size: 197 926 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 3066 3251 513 Size: 552 2001 185 3074 Fragments arranged by size: 3074 2001 552 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 3016 3839 3856 4009 4194 4639 Size: 1255 761 675 823 17 153 185 445 Cuts at: 4639 325 Size: 1498 Fragments arranged by size: 1498 1255 823 761 675 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2331 2922 Size: 274 80 33 51 1443 189 165 591 Cuts at: 2922 4058 5770 96 Size: 1136 1712 138 Fragments arranged by size: 1712 1443 1136 591 274 189 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2688 2720 5772 5804 2688 Size: 32 3052 32 2696 Fragments arranged by size: 3052 2696 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2969 1316 Size: 1653 4159 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2434 2434 Size: 5812 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 2667 3137 1870 Size: 797 470 4545 Fragments arranged by size: 4545 797 470 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI