Restriction map for
pFN21AB7421
(Linear) (Six-base) MAPSORT of: KIBB7421.seq Check: 8216 from: 1 to: 1107
KIBB7421.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1072 1107
Size: 724 348 35
Fragments arranged by size:
724 348 35
AflIII A'CryG_T
Cuts at: 0 72 1107
Size: 72 1035
AleI CACnn'nnGTG
Cuts at: 0 379 1107
Size: 379 728
ApaLI G'TGCA_C
Cuts at: 0 438 1107
Size: 438 669
ApoI r'AATT_y
Cuts at: 0 481 1107
Size: 481 626
AseI AT'TA_AT
Cuts at: 0 65 1107
Size: 65 1042
AvaI C'yCGr_G
Cuts at: 0 662 1107
Size: 662 445
BanI G'GyrC_C
Cuts at: 0 690 1107
Size: 690 417
BanII G_rGCy'C
Cuts at: 0 607 838 1050 1107
Size: 607 231 212 57
Fragments arranged by size:
607 231 212 57
BbvCI CC'TCA_GC
Cuts at: 0 337 1107
Size: 337 770
Bme1580I G_kGCm'C
Cuts at: 0 442 1107
Size: 442 665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1107
Size: 1064 43
Bpu10I CC'TnA_GC
Cuts at: 0 337 1107
Size: 337 770
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 1107
Size: 325 782
BseYI C'CCAG_C
Cuts at: 0 492 1107
Size: 492 615
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1107
Size: 737 370
BsiHKAI G_wGCw'C
Cuts at: 0 442 838 1050 1107
Size: 442 396 212 57
Fragments arranged by size:
442 396 212 57
BsmI GAATG_Cn'
Cuts at: 0 103 1107
Size: 103 1004
Bsp1286I G_dGCh'C
Cuts at: 0 442 607 838 1050 1107
Size: 442 165 231 212 57
Fragments arranged by size:
442 231 212 165 57
BspHI T'CATG_A
Cuts at: 0 554 1107
Size: 554 553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 413 577 1107
Size: 413 164 530
Fragments arranged by size:
530 413 164
BsrGI T'GTAC_A
Cuts at: 0 917 1107
Size: 917 190
Bsu36I CC'TnA_GG
Cuts at: 0 219 1107
Size: 219 888
DraI TTT'AAA
Cuts at: 0 23 44 1107
Size: 23 21 1063
Fragments arranged by size:
1063 23 21
DrdI GACnn_nn'nnGTC
Cuts at: 0 891 1107
Size: 891 216
EaeI y'GGCC_r
Cuts at: 0 269 521 1107
Size: 269 252 586
Fragments arranged by size:
586 269 252
EarI CTCTTCn'nnn_
Cuts at: 0 152 1048 1107
Size: 152 896 59
Fragments arranged by size:
896 152 59
EcoICRI GAG'CTC
Cuts at: 0 836 1048 1107
Size: 836 212 59
Fragments arranged by size:
836 212 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1064 1072 1107
Size: 724 340 8 35
Fragments arranged by size:
724 340 35 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1065 1107
Size: 1065 42
EcoO109I rG'GnC_Cy
Cuts at: 0 1058 1078 1107
Size: 1058 20 29
Fragments arranged by size:
1058 29 20
EcoRI G'AATT_C
Cuts at: 0 481 1107
Size: 481 626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1077 1107
Size: 1077 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 533 565 1032 1064 1107
Size: 533 32 467 32 43
Fragments arranged by size:
533 467 43 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1107
Size: 553 554
MscI TGG'CCA
Cuts at: 0 271 523 1107
Size: 271 252 584
Fragments arranged by size:
584 271 252
MslI CAynn'nnrTG
Cuts at: 0 190 379 637 886 925 997 1107
Size: 190 189 258 249 39 72 110
Fragments arranged by size:
258 249 190 189 110 72 39
NspI r_CATG'y
Cuts at: 0 76 716 930 1107
Size: 76 640 214 177
Fragments arranged by size:
640 214 177 76
PasI CC'CwG_GG
Cuts at: 0 92 1107
Size: 92 1015
PciI A'CATG_T
Cuts at: 0 72 1107
Size: 72 1035
PpuMI rG'GwC_Cy
Cuts at: 0 1078 1107
Size: 1078 29
SacI G_AGCT'C
Cuts at: 0 838 1050 1107
Size: 838 212 57
Fragments arranged by size:
838 212 57
SfcI C'TryA_G
Cuts at: 0 143 176 785 818 1017 1107
Size: 143 33 609 33 199 90
Fragments arranged by size:
609 199 143 90 33 33
SmaI CCC'GGG
Cuts at: 0 664 1107
Size: 664 443
StyI C'CwwG_G
Cuts at: 0 1061 1107
Size: 1061 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 1011 1107
Size: 336 675 96
Fragments arranged by size:
675 336 96
TatI w'GTAC_w
Cuts at: 0 161 326 917 1107
Size: 161 165 591 190
Fragments arranged by size:
591 190 165 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 683 715 1107
Size: 683 32 392
Fragments arranged by size:
683 392 32
Tth111I GACn'n_nGTC
Cuts at: 0 964 1107
Size: 964 143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 1107
Size: 429 678
XmaI C'CCGG_G
Cuts at: 0 662 1107
Size: 662 445
Enzymes that do cut:
AcuI AflIII AleI ApaLI ApoI AseI AvaI BanI
BanII BbvCI Bme1580I BpmI Bpu10I BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI Bsu36I DraI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I MmeI MscI MslI NspI PasI PciI
PpuMI SacI SfcI SmaI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraIII EagI EciI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmnI
ZraI