Restriction map for
pFN21AB7420
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeI
MaeII MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AlwNI 794 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 166 AGCGCT AGC^GCT
AscI 149 GGCGCGCC GG^CGCGCC
AspHI 610 GAGCTC G[AT]GC[AT]^C
BanII 610 GAGCTC G[GA]GC[CT]^C
BbeI 629 GGCGCC GGCGC^C
BbsI 424 GTCTTC GAAGAC(2/6)
BbvCI 510 GCTGAGG CCTCAGC(-5/-2)
BglII 36 AGATCT A^GATCT
BpuAI 424 GTCTTC GAAGAC(2/6)
BsaBI 762 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 50 TCCGGT [AT]^CCGG[AT]
BsgI 289 CTGCAC GTGCAG(16/14)
BsiHKAI 610 GAGCTC G[AT]GC[AT]^C
BspMI 377 ACCTGC ACCTGC(4/8)
BsrFI 456 GCCGGC [GA]^CCGG[CT]
BssHII 150 GCGCGC G^CGCGC
BstF5I 402 CATCC GGATG(2/0)
BstXI 15 CCAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 456 GCCGGC [GA]^CCGG[CT]
DrdI 697 GACTGCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 434 CCACGG C^C[GA][CT]GG
EarI 730 CTCTTC CTCTTC(1/4)
Eco47III 166 AGCGCT AGC^GCT
EcoT14I 486 CCTTGG C^C[AT][AT]GG
FokI 402 CATCC GGATG(9/13)
HgaI 306 GACGC GACGC(5/10)
HinfI 676 GACTC G^A[ACGT]TC
KasI 629 GGCGCC G^GCGCC
Ksp632I 730 CTCTTC CTCTTC(1/4)
MamI 762 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 86 CCGCTG C[AC]G^C[GT]G
NaeI 456 GCCGGC GCC^GGC
NarI 629 GGCGCC GG^CGCC
NgoMIV 456 GCCGGC G^CCGGC
PleI 676 GACTC GAGTC(4/5)
PshAI 777 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 610 GAGCTC GAGCT^C
SapI 729 GCTCTTC GCTCTTC(1/4)
SfaNI 561 GATGC GCATC(5/9)
SfoI 629 GGCGCC GGC^GCC
StyI 486 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 306 GACGCC G[GA]^CG[CT]C
629 GGCGCC
AlwI 387 GATCC GGATC(4/5)
467 GGATC
AvaII 572 GGACC G^G[AT]CC
783 GGTCC
BalI 431 TGGCCA TGG^CCA
489 TGGCCA
BsaHI 306 GACGCC G[GA]^CG[CT]C
629 GGCGCC
BstYI 36 AGATCT [GA]^GATC[CT]
386 AGATCC
Cfr13I 572 GGACC G^G[ACGT]CC
783 GGTCC
DraII 571 GGGACCC [GA]G^G[ACGT]CC[CT]
782 AGGTCCT
EaeI 431 TGGCCA [CT]^GGCC[GA]
489 TGGCCA
EcoO109I 571 GGGACCC [GA]G^G[ACGT]CC[CT]
782 AGGTCCT
FauI 451 CCCGC CCCGC(4/6)
283 GCGGG
HaeII 166 AGCGCT [GA]GCGC^[CT]
629 GGCGCC
Hin1I 306 GACGCC G[GA]^CG[CT]C
629 GGCGCC
HincII 112 GTCAAC GT[CT]^[GA]AC
397 GTTGAC
HindII 112 GTCAAC GT[CT]^[GA]AC
397 GTTGAC
MaeIII 157 GTCAC ^GT[ACGT]AC
304 GTGAC
MboII 425 TCTTC GAAGA(8/7)
731 TCTTC
MflI 36 AGATCT [GA]^GATC[CT]
386 AGATCC
MluNI 431 TGGCCA TGG^CCA
489 TGGCCA
MscI 431 TGGCCA TGG^CCA
489 TGGCCA
MslI 435 CACGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
555 CACCATGATG
NspI 320 GCATGT [GA]CATG^[CT]
440 ACATGC
PpuMI 571 GGGACCC [GA]G^G[AT]CC[CT]
782 AGGTCCT
Sau96I 572 GGACC G^G[ACGT]CC
783 GGTCC
SmlI 634 CTCAAG C^T[CT][GA]AG
664 CTTGAG
Tsp45I 157 GTCAC ^GT[GC]AC
304 GTGAC
XhoII 36 AGATCT [GA]^GATC[CT]
386 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI DdeI
DpnII EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI ScrFI TseI TspRI