Restriction map for
pFN21AB7420
(Circular) (Six-base) MAPSORT of: pFN21AB7420.seq Check: 6327 from: 1 to: 5512
pFN21AB7420 5512 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2391 2391
Size: 5512
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3575 4959 5227 1632
Size: 1943 1384 268 1917
Fragments arranged by size:
1943 1917 1384 268
AfeI AGC'GCT
Cuts at: 2173 2173
Size: 5512
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 2804 3255 4827 2804
Size: 451 1572 3489
Fragments arranged by size:
3489 1572 451
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AscI GG'CGCG_CC
Cuts at: 2155 2155
Size: 5512
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 1870
Size: 73 894 4545
Fragments arranged by size:
4545 894 73
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 2541 2634 2833 4288
Size: 325 217 627 587 167 93 199 1455
Cuts at: 4288 618
Size: 1842
Fragments arranged by size:
1842 1455 627 587 325 217 199 167
93
BanII G_rGCy'C
Cuts at: 1391 2619 2831 1391
Size: 1228 212 4072
Fragments arranged by size:
4072 1228 212
BbeI G_GCGC'C
Cuts at: 2638 2638
Size: 5512
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2422 928
Size: 971 523 4018
Fragments arranged by size:
4018 971 523
BbvCI CC'TCA_GC
Cuts at: 2516 2516
Size: 5512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3703 3737 1903
Size: 34 50 34 1682 34 3678
Fragments arranged by size:
3678 1682 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4123 136
Size: 107 122 71 631 605 2451 1525
Fragments arranged by size:
2451 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2041 5507 2041
Size: 3466 2046
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 2379 2544 3573 4729 1790
Size: 589 165 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 589 165
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2014 2542 3243
Size: 416 53 83 399 24 597 528 701
Cuts at: 3243 4201 442
Size: 958 1753
Fragments arranged by size:
1753 958 701 597 528 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 2384 2396 2633 4172 1239
Size: 921 224 12 237 1539 2579
Fragments arranged by size:
2579 1539 921 237 224 12
Bpu10I CC'TnA_GC
Cuts at: 2516 2516
Size: 5512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2690 3637 4502 4800 2624
Size: 66 947 865 298 3336
Fragments arranged by size:
3336 947 865 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4175 882
Size: 617 2676 2219
Fragments arranged by size:
2676 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 2771 5506 2771
Size: 2735 2777
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2312 2635 3319 3701
Size: 53 83 186 936 779 323 684 382
Cuts at: 3701 4336 275
Size: 635 1451
Fragments arranged by size:
1451 936 779 684 635 382 323 186
83 53
BsaWI w'CCGG_w
Cuts at: 2055 3945 4343 4617 4764 5175 2055
Size: 1890 398 274 147 411 2392
Fragments arranged by size:
2392 1890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 1178
Size: 30 165 30 789 30 4468
Fragments arranged by size:
4468 789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5512
BseYI C'CCAG_C
Cuts at: 4715 5211 4715
Size: 496 5016
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 1163
Size: 573 543 4396
Fragments arranged by size:
4396 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3723 4751 1540
Size: 275 186 961 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2619 3573 3658 4729 2619
Size: 954 85 1071 3402
Fragments arranged by size:
3402 1071 954 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2379 2544 2619 2831 3573 3658 4729
Size: 399 589 165 75 212 742 85 1071
Cuts at: 4729 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 589 399 212 165 85
75
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2867 844
Size: 1547 476 3489
Fragments arranged by size:
3489 1547 476
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4007 4181 65
Size: 1183 2759 174 1396
Fragments arranged by size:
2759 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2461 4156 4343 1700
Size: 110 651 1695 187 2869
Fragments arranged by size:
2869 1695 651 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 2155 1925
Size: 230 5282
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5512
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstXI CCAn_nnnn'nTGG
Cuts at: 2027 2027
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2041 2391 2842 3209 3594 3611 5063 5149
Size: 69 350 451 367 385 17 1452 86
Cuts at: 5149 5161 5507 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 451 385 367 350 346 86
69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2439 513
Size: 552 1374 3586
Fragments arranged by size:
3586 1374 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 2218 3028 3822 3842 2218
Size: 810 794 20 3888
Fragments arranged by size:
3888 810 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2816 2816
Size: 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2708 4519 809
Size: 507 1392 1811 1802
Fragments arranged by size:
1811 1802 1392 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2436 2494 2959
Size: 54 1425 50 275 18 606 58 465
Cuts at: 2959 3848 5477 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 606 465 275 58 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2959 1537
Size: 275 1147 4090
Fragments arranged by size:
4090 1147 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2741 3442 1353
Size: 1388 701 3423
Fragments arranged by size:
3423 1388 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2237 4099 4473 4619 387
Size: 1064 138 648 1862 374 146 1280
Fragments arranged by size:
1862 1280 1064 648 374 146 138
EcoICRI GAG'CTC
Cuts at: 2617 2617
Size: 5512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2384 2396 2633 3575 4172 4959
Size: 393 528 224 12 237 942 597 787
Cuts at: 4959 5227 1239
Size: 268 1524
Fragments arranged by size:
1524 942 787 597 528 393 268 237
224 12
EcoO109I rG'GnC_Cy
Cuts at: 2577 2788 2577
Size: 211 5301
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 2175 2638 4659 1678
Size: 497 463 2021 2531
Fragments arranged by size:
2531 2021 497 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2409 2441 4153 4185 4227
Size: 32 135 32 837 32 1712 32 42
Cuts at: 4227 4259 1373
Size: 32 2626
Fragments arranged by size:
2626 1712 837 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2119 2404 2856 3102 669
Size: 988 281 181 285 452 246 3079
Fragments arranged by size:
3079 988 452 285 281 246 181
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KasI G'GCGC_C
Cuts at: 2634 2634
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4626 4810 1305
Size: 3321 184 2007
Fragments arranged by size:
3321 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2438 2496 10
Size: 54 1425 343 606 58 3026
Fragments arranged by size:
3026 1425 606 343 58 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 2564 3470 3829 3988 518
Size: 648 1278 120 906 359 159 2042
Fragments arranged by size:
2042 1278 906 648 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2093 2928 3605 4753 4998 1606
Size: 487 835 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 487 245
NaeI GCC'GGC
Cuts at: 2463 2463
Size: 5512
NarI GG'CG_CC
Cuts at: 2635 2635
Size: 5512
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4960
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NgoMIV G'CCGG_C
Cuts at: 2461 2461
Size: 5512
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2329 2449 2870 4415 1132
Size: 97 1100 120 421 1545 2229
Fragments arranged by size:
2229 1545 1100 421 120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5512
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2577 2788 2577
Size: 211 5301
PshAI GACnn'nnGTC
Cuts at: 1613 2786 1613
Size: 1173 4339
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PstI C_TGCA'G
Cuts at: 830 2864 830
Size: 2034 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
SacI G_AGCT'C
Cuts at: 2619 2619
Size: 5512
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SanDI GG'GwC_CC
Cuts at: 2577 2577
Size: 5512
SapI GCTCTTCn'nnn_
Cuts at: 2741 2741
Size: 5512
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2860 3995 4676 4867 826
Size: 219 1815 1135 681 191 1471
Fragments arranged by size:
1815 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2636 2636
Size: 5512
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 2669 3616 4517 4779 820
Size: 197 926 696 30 947 901 262 1553
Fragments arranged by size:
1553 947 926 901 696 262 197 30
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2491 2951 513
Size: 552 1426 460 3074
Fragments arranged by size:
3074 1426 552 460
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 3539 3556 3709 3894 4339 325
Size: 1255 1020 939 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1020 939 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3758 5470 96
Size: 274 80 33 51 1443 1781 1712 138
Fragments arranged by size:
1781 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2192 2224 5472 5504 2192
Size: 32 3248 32 2200
Fragments arranged by size:
3248 2200 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SexAI SfiI SrfI SwaI XcmI