Restriction map for
pFN21AB7419
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BmyI BpmI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI Ksp632I KspI MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 757 GGTACC G^GTACC
AcsI 31 GAATTC [GA]^AATT[CT]
ApoI 31 GAATTC [GA]^AATT[CT]
AseI 520 ATTAAT AT^TAAT
AsnI 520 ATTAAT AT^TAAT
Asp718I 757 GGTACC G^GTACC
AvaII 795 GGACC G^G[AT]CC
BalI 371 TGGCCA TGG^CCA
BanI 757 GGTACC G^G[CT][GA]CC
BbvI 913 GCTGC GCAGC(8/12)
BglII 539 AGATCT A^GATCT
BsaAI 103 TACGTA [CT]AC^GT[GA]
BseRI 225 CTCCTC GAGGAG(10/8)
BspHI 404 TCATGA T^CATGA
BsrI 243 ACTGG ACTGG(1/-1)
BssSI 634 CTCGTG CACGAG(-5/-1)
BstEII 55 GGTTACC G^GT[ACGT]ACC
BstPI 55 GGTTACC G^GT[ACGT]ACC
BstYI 539 AGATCT [GA]^GATC[CT]
Cfr13I 795 GGACC G^G[ACGT]CC
DraII 794 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 794 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 55 GGTTACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
EcoRV 208 GATATC GAT^ATC
Fnu4HI 913 GCTGC GC^[ACGT]GC
HincII 324 GTCAAC GT[CT]^[GA]AC
HindII 324 GTCAAC GT[CT]^[GA]AC
ItaI 913 GCTGC GC^[ACGT]GC
KpnI 757 GGTACC GGTAC^C
MaeII 104 ACGT A^CGT
MflI 539 AGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
NlaIV 757 GGTACC GG[ACGT]^[ACGT]CC
PflMI 488 CCATGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 47 GACTC GAGTC(4/5)
PpuMI 794 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 520 ATTAAT AT^TAAT
RcaI 404 TCATGA T^CATGA
Sau96I 795 GGACC G^G[ACGT]CC
SfaNI 146 GCATC GCATC(5/9)
SnaBI 103 TACGTA TAC^GTA
TseI 913 GCTGC G^C[AT]GC
Tsp45I 859 GTGAC ^GT[GC]AC
Van91I 488 CCATGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 792 CCAGGACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 539 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbsI 698 GAAGAC GAAGAC(2/6)
161 GTCTTC
BclI 134 TGATCA T^GATCA
743 TGATCA
BlpI 722 GCTCAGC GC^T[ACGT]AGC
930 GCTGAGC
Bpu1102I 722 GCTCAGC GC^T[ACGT]AGC
930 GCTGAGC
BpuAI 698 GAAGAC GAAGAC(2/6)
161 GTCTTC
BsgI 290 GTGCAG GTGCAG(16/14)
295 GTGCAG
BsmFI 45 GGGAC GGGAC(10/14)
789 GTCCC
BsrDI 122 GCAATG GCAATG(2/0)
316 GCAATG
BstF5I 660 CATCC GGATG(2/0)
286 GGATG
Cac8I 142 GCAAGC GC[ACGT]^[ACGT]GC
819 GCAAGC
CelII 722 GCTCAGC GC^T[ACGT]AGC
930 GCTGAGC
EaeI 371 TGGCCA [CT]^GGCC[GA]
689 CGGCCA
EcoT14I 342 CCTTGG C^C[AT][AT]GG
801 CCTTGG
FbaI 134 TGATCA T^GATCA
743 TGATCA
FokI 660 CATCC GGATG(9/13)
286 GGATG
MslI 168 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CATCATCATG
NspI 167 ACATGC [GA]CATG^[CT]
718 ACATGC
SfcI 824 CTACAG C^T[GA][CT]AG
885 CTATAG
SmlI 67 CTTGAG C^T[CT][GA]AG
891 CTTGAG
StyI 342 CCTTGG C^C[AT][AT]GG
801 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI Asp700I BfaI BsaJI
BsiYI BslI BssKI BstNI DdeI
DpnII EcoRII HaeIII HinfI HphI
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI SspI TfiI Tru9I
Tsp509I TspRI XmnI