(Circular) (Six-base) MAPSORT of: pFN21AB7418.seq Check: 5485 from: 1 to: 5836 pFN21AB7418 5836 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3899 5283 5551 1632 Size: 2267 1384 268 1917 Fragments arranged by size: 2267 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3110 3142 3110 Size: 32 5804 AlwNI CAG_nnn'CTG Cuts at: 2524 2764 3579 5151 2524 Size: 240 815 1572 3209 Fragments arranged by size: 3209 1572 815 240 ApaI G_GGCC'C Cuts at: 2012 2121 2446 2012 Size: 109 325 5402 Fragments arranged by size: 5402 325 109 ApaLI G'TGCA_C Cuts at: 2800 3893 5049 2800 Size: 1093 1156 3587 Fragments arranged by size: 3587 1156 1093 ApoI r'AATT_y Cuts at: 1493 3101 3145 3371 3523 5632 1493 Size: 1608 44 226 152 2109 1697 Fragments arranged by size: 2109 1697 1608 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2120 3161 1870 Size: 73 177 1041 4545 Fragments arranged by size: 4545 1041 177 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2260 2317 2456 3157 4612 Size: 325 217 627 473 57 139 701 1455 Cuts at: 4612 618 Size: 1842 Fragments arranged by size: 1842 1455 701 627 473 325 217 139 57 BanII G_rGCy'C Cuts at: 1391 2012 2121 2176 2446 2859 3155 1391 Size: 621 109 55 270 413 296 4072 Fragments arranged by size: 4072 621 413 296 270 109 55 BbeI G_GCGC'C Cuts at: 2264 2264 Size: 5836 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2791 2825 4027 4061 1903 Size: 34 854 34 1202 34 3678 Fragments arranged by size: 3678 1202 854 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BfrBI ATG'CAT Cuts at: 2582 2582 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2101 2258 2727 Size: 107 122 71 631 605 429 157 469 Cuts at: 2727 4447 136 Size: 1720 1525 Fragments arranged by size: 1720 1525 631 605 469 429 157 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 2933 3253 2933 Size: 320 5516 Bme1580I G_kGCm'C Cuts at: 1790 2012 2121 2446 2740 2804 3897 5053 1790 Size: 222 109 325 294 64 1093 1156 2573 Fragments arranged by size: 2573 1156 1093 325 294 222 109 64 BmgBI CAC'GTC Cuts at: 1318 2194 1318 Size: 876 4960 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2365 3567 4525 Size: 416 53 83 399 24 948 1202 958 Cuts at: 4525 442 Size: 1753 Fragments arranged by size: 1753 1202 958 948 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 3062 4496 1239 Size: 1007 816 1434 2579 Fragments arranged by size: 2579 1434 1007 816 Bpu10I CC'TnA_GC Cuts at: 2528 2528 Size: 5836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2897 3961 4826 5124 2897 Size: 1064 865 298 3609 Fragments arranged by size: 3609 1064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2966 4499 882 Size: 617 1467 1533 2219 Fragments arranged by size: 2219 1533 1467 617 BsaAI yAC'GTr Cuts at: 493 1204 2350 3627 493 Size: 711 1146 1277 2702 Fragments arranged by size: 2702 1277 1146 711 BsaBI GATnn'nnATC Cuts at: 2294 5830 2294 Size: 3536 2300 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2261 2834 3643 Size: 53 83 186 936 699 29 573 809 Cuts at: 3643 4025 4660 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 809 699 635 573 382 186 83 53 29 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2853 2883 3000 3030 3110 Size: 30 165 30 1450 30 117 30 80 Cuts at: 3110 3140 1178 Size: 30 3874 Fragments arranged by size: 3874 1450 165 117 80 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5836 BseYI C'CCAG_C Cuts at: 2104 2269 2517 2823 5039 5535 2104 Size: 165 248 306 2216 496 2405 Fragments arranged by size: 2405 2216 496 306 248 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2154 2278 3286 4047 5075 1540 Size: 275 186 153 124 1008 761 1028 2301 Fragments arranged by size: 2301 1028 1008 761 275 186 153 124 BsiHKAI G_wGCw'C Cuts at: 2804 2859 3897 3982 5053 2804 Size: 55 1038 85 1071 3587 Fragments arranged by size: 3587 1071 1038 85 55 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 BsmBI CGTCTCn'nnnn_ Cuts at: 2070 2070 Size: 5836 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2121 2176 2446 2740 2804 2859 Size: 399 222 109 55 270 294 64 55 Cuts at: 2859 3155 3897 3982 5053 1391 Size: 296 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 296 294 270 222 109 85 64 55 55 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3095 3191 844 Size: 2251 96 3489 Fragments arranged by size: 3489 2251 96 BsrBI CCG'CTC Cuts at: 1394 1823 2018 3723 1394 Size: 429 195 1705 3507 Fragments arranged by size: 3507 1705 429 195 BsrDI GCAATG_nn' Cuts at: 65 1248 2582 4331 4505 65 Size: 1183 1334 1749 174 1396 Fragments arranged by size: 1749 1396 1334 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2146 2928 4480 4667 1700 Size: 110 336 782 1552 187 2869 Fragments arranged by size: 2869 1552 782 336 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 2810 3038 3896 4908 2810 Size: 228 858 1012 3738 Fragments arranged by size: 3738 1012 858 228 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5836 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2575 2782 2422 Size: 153 207 5476 Fragments arranged by size: 5476 207 153 BstYI r'GATC_y Cuts at: 1972 2959 3166 3533 3918 3935 5387 5473 5485 Size: 987 207 367 385 17 1452 86 12 Cuts at: 5485 5831 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 987 385 367 346 207 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 2604 513 Size: 552 939 123 477 3745 Fragments arranged by size: 3745 939 552 477 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2346 2401 3352 4146 4166 2346 Size: 55 951 794 20 4016 Fragments arranged by size: 4016 951 794 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DraIII CAC_nnn'GTG Cuts at: 2494 2494 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2219 4843 809 Size: 507 903 2624 1802 Fragments arranged by size: 2624 1802 903 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2787 3283 Size: 54 1425 50 275 18 321 636 496 Cuts at: 3283 4172 5801 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 636 496 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2151 3283 1537 Size: 275 339 1132 4090 Fragments arranged by size: 4090 1132 339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2453 3766 1353 Size: 1100 1313 3423 Fragments arranged by size: 3423 1313 1100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2040 2147 2184 4423 4797 4943 Size: 1064 138 451 107 37 2239 374 146 Cuts at: 4943 387 Size: 1280 Fragments arranged by size: 2239 1280 1064 451 374 146 138 107 37 EcoICRI GAG'CTC Cuts at: 2857 2857 Size: 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 3062 3899 4496 5283 5551 1239 Size: 393 614 816 837 597 787 268 1524 Fragments arranged by size: 1524 837 816 787 614 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2684 2684 Size: 5836 EcoO109I rG'GnC_Cy Cuts at: 2009 2117 2419 2443 2595 2950 2009 Size: 108 302 24 152 355 4895 Fragments arranged by size: 4895 355 302 152 108 24 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2677 2677 Size: 5836 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2264 2887 2926 4983 1678 Size: 586 623 39 2057 2531 Fragments arranged by size: 2531 2057 623 586 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2618 2650 4477 4509 4551 Size: 32 135 32 1046 32 1827 32 42 Cuts at: 4551 4583 1373 Size: 32 2626 Fragments arranged by size: 2626 1827 1046 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2658 3180 3426 669 Size: 988 281 720 522 246 3079 Fragments arranged by size: 3079 988 720 522 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KasI G'GCGC_C Cuts at: 2260 2260 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 4950 5134 1305 Size: 1251 2394 184 2007 Fragments arranged by size: 2394 2007 1251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2789 10 Size: 54 1425 343 957 3057 Fragments arranged by size: 3057 1425 957 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3794 4153 4312 518 Size: 648 2628 359 159 2042 Fragments arranged by size: 2628 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2098 2129 2622 2764 2827 2851 3252 Size: 465 27 31 493 142 63 24 401 Cuts at: 3252 3929 5077 5322 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 493 465 401 245 142 63 31 27 24 NaeI GCC'GGC Cuts at: 2148 2930 2148 Size: 782 5054 NarI GG'CG_CC Cuts at: 2261 2261 Size: 5836 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NgoMIV G'CCGG_C Cuts at: 2146 2928 2146 Size: 782 5054 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NsiI A_TGCA'T Cuts at: 2584 2584 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2494 1108 Size: 1386 4450 PfoI T'CCnGG_A Cuts at: 2223 2223 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3110 3142 3905 3937 5418 5450 3110 Size: 32 763 32 1481 32 3496 Fragments arranged by size: 3496 1481 763 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2595 2595 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PspOMI G'GGCC_C Cuts at: 2008 2117 2442 2008 Size: 109 325 5402 Fragments arranged by size: 5402 325 109 PstI C_TGCA'G Cuts at: 830 2592 2760 3188 830 Size: 1762 168 428 3478 Fragments arranged by size: 3478 1762 428 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2764 2764 Size: 5836 SacI G_AGCT'C Cuts at: 2859 2859 Size: 5836 SacII CC_GC'GG Cuts at: 2130 2130 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2588 2756 3184 4319 5000 5191 826 Size: 219 1543 168 428 1135 681 191 1471 Fragments arranged by size: 1543 1471 1135 681 428 219 191 168 SfoI GGC'GCC Cuts at: 2262 2262 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 2122 3163 1872 Size: 250 1041 4545 Fragments arranged by size: 4545 1041 250 SmlI C'TyrA_G Cuts at: 820 1017 1943 2912 3940 4841 5103 820 Size: 197 926 969 1028 901 262 1553 Fragments arranged by size: 1553 1028 969 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 2408 2808 1517 Size: 891 400 4545 Fragments arranged by size: 4545 891 400 StyI C'CwwG_G Cuts at: 513 1065 2004 2415 2685 2722 3275 513 Size: 552 939 411 270 37 553 3074 Fragments arranged by size: 3074 939 553 552 411 270 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2444 3863 3880 4033 4218 4663 325 Size: 1255 864 1419 17 153 185 445 1498 Fragments arranged by size: 1498 1419 1255 864 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2659 2691 2853 2885 5796 5828 2659 Size: 32 162 32 2911 32 2667 Fragments arranged by size: 2911 2667 162 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2714 1316 Size: 1398 4438 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2328 2782 2328 Size: 454 5382 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 2120 3161 1870 Size: 250 1041 4545 Fragments arranged by size: 4545 1041 250 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI