(Circular) (Six-base) MAPSORT of: pFN21AB7414.seq Check: 4348 from: 1 to: 5971 pFN21AB7414 5971 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2323 2686 2854 3190 4034 5418 5686 1632 Size: 691 363 168 336 844 1384 268 1917 Fragments arranged by size: 1917 1384 844 691 363 336 268 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 2661 1166 Size: 1495 4476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 3714 5286 3714 Size: 1572 4399 ApaI G_GGCC'C Cuts at: 2107 2107 Size: 5971 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 3072 3280 3506 3658 5767 1493 Size: 1579 208 226 152 2109 1697 Fragments arranged by size: 2109 1697 1579 226 208 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3296 1870 Size: 73 1353 4545 Fragments arranged by size: 4545 1353 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2022 2475 3292 4747 618 Size: 325 217 627 235 453 817 1455 1842 Fragments arranged by size: 1842 1455 817 627 453 325 235 217 BanII G_rGCy'C Cuts at: 1391 2107 3290 1391 Size: 716 1183 4072 Fragments arranged by size: 4072 1183 716 BbeI G_GCGC'C Cuts at: 2026 2026 Size: 5971 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2878 3260 928 Size: 971 979 382 3639 Fragments arranged by size: 3639 979 971 382 BbvCI CC'TCA_GC Cuts at: 2467 2467 Size: 5971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2107 4032 5188 1790 Size: 317 1925 1156 2573 Fragments arranged by size: 2573 1925 1156 317 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2922 3006 3090 Size: 416 53 83 399 24 1505 84 84 Cuts at: 3090 3702 4660 442 Size: 612 958 1753 Fragments arranged by size: 1753 1505 958 612 416 399 84 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2213 2850 2876 4631 1239 Size: 908 66 637 26 1755 2579 Fragments arranged by size: 2579 1755 908 637 66 26 Bpu10I CC'TnA_GC Cuts at: 2467 2467 Size: 5971 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2950 4096 4961 5259 2950 Size: 1146 865 298 3662 Fragments arranged by size: 3662 1146 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2188 4634 882 Size: 617 689 2446 2219 Fragments arranged by size: 2446 2219 689 617 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2023 3778 4160 4795 Size: 53 83 186 936 490 1755 382 635 Cuts at: 4795 275 Size: 1451 Fragments arranged by size: 1755 1451 936 635 490 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5746 Fragments arranged by size: 5746 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2461 1186 Size: 1275 4696 BseYI C'CCAG_C Cuts at: 2187 2376 5174 5670 2187 Size: 189 2798 496 2488 Fragments arranged by size: 2798 2488 496 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 1163 Size: 573 381 5017 Fragments arranged by size: 5017 573 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4032 4117 5188 4032 Size: 85 1071 4815 Fragments arranged by size: 4815 1071 85 BsmI GAATG_Cn' Cuts at: 2156 2684 2994 3119 3482 3575 2156 Size: 528 310 125 363 93 4552 Fragments arranged by size: 4552 528 363 310 125 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 3290 4032 4117 5188 1391 Size: 399 317 1183 742 85 1071 2174 Fragments arranged by size: 2174 1183 1071 742 399 317 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 844 Size: 2482 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4466 4640 65 Size: 1183 3218 174 1396 Fragments arranged by size: 3218 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 2109 96 Size: 2013 3958 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966 Size: 1329 367 385 17 1452 86 12 346 Cuts at: 5966 1972 Size: 1977 Fragments arranged by size: 1977 1452 1329 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2210 513 Size: 552 1145 4274 Fragments arranged by size: 4274 1145 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2671 2706 529 Size: 35 2107 35 3794 Fragments arranged by size: 3794 2107 35 35 DraI TTT'AAA Cuts at: 2761 3275 2761 Size: 514 5457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936 Size: 54 1425 50 275 18 1588 889 1629 Cuts at: 5936 8 Size: 43 Fragments arranged by size: 1629 1588 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3901 1353 Size: 2548 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2213 2323 2686 2850 2854 2876 Size: 393 515 66 110 363 164 4 22 Cuts at: 2876 3190 4034 4631 5418 5686 1239 Size: 314 844 597 787 268 1524 Fragments arranged by size: 1524 844 787 597 515 393 363 314 268 164 110 66 22 4 EcoNI CCTnn'n_nnAGG Cuts at: 2470 2470 Size: 5971 EcoO109I rG'GnC_Cy Cuts at: 2103 2104 2215 2356 2103 Size: 1 111 141 5718 Fragments arranged by size: 5718 141 111 1 EcoRI G'AATT_C Cuts at: 3072 3280 3658 3072 Size: 208 378 5385 Fragments arranged by size: 5385 378 208 EcoRV GAT'ATC Cuts at: 2586 2586 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 2026 5118 1678 Size: 348 3092 2531 Fragments arranged by size: 3092 2531 348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 2797 2829 3049 Size: 32 135 32 731 32 462 32 220 Cuts at: 3049 3081 4612 4644 4686 4718 1373 Size: 32 1531 32 42 32 2626 Fragments arranged by size: 2626 1531 731 462 220 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KasI G'GCGC_C Cuts at: 2022 2022 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2924 3069 5085 5269 1305 Size: 1619 145 2016 184 2007 Fragments arranged by size: 2016 2007 1619 184 145 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2624 2643 2661 2761 3929 4288 4447 Size: 648 1458 19 18 100 1168 359 159 Cuts at: 4447 518 Size: 2042 Fragments arranged by size: 2042 1458 1168 648 359 159 100 19 18 MspA1I CmG'CkG Cuts at: 1606 2191 3210 3387 4064 5212 5457 1606 Size: 585 1019 177 677 1148 245 2120 Fragments arranged by size: 2120 1148 1019 677 585 245 177 NarI GG'CG_CC Cuts at: 2023 2023 Size: 5971 NcoI C'CATG_G Cuts at: 513 1065 2210 513 Size: 552 1145 4274 Fragments arranged by size: 4274 1145 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3329 4874 1132 Size: 97 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 97 PasI CC'CwG_GG Cuts at: 1348 2226 1348 Size: 878 5093 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3260 1613 Size: 1647 4324 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PspOMI G'GGCC_C Cuts at: 2103 2103 Size: 5971 PstI C_TGCA'G Cuts at: 830 3323 830 Size: 2493 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2191 3210 2191 Size: 1019 4952 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 3319 4454 5135 5326 826 Size: 219 2274 1135 681 191 1471 Fragments arranged by size: 2274 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2024 2024 Size: 5971 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2965 4075 4976 5238 820 Size: 197 926 1022 1110 901 262 1553 Fragments arranged by size: 1553 1110 1022 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 2533 3895 5 Size: 47 2481 1362 2081 Fragments arranged by size: 2481 2081 1362 47 StuI AGG'CCT Cuts at: 1517 2486 2867 1517 Size: 969 381 4621 Fragments arranged by size: 4621 969 381 StyI C'CwwG_G Cuts at: 513 1065 2210 3410 513 Size: 552 1145 1200 3074 Fragments arranged by size: 3074 1200 1145 552 SwaI ATTT'AAAT Cuts at: 2761 2761 Size: 5971 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2081 3998 4015 4168 4353 4798 325 Size: 1255 501 1917 17 153 185 445 1498 Fragments arranged by size: 1917 1498 1255 501 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2058 2109 4217 Size: 274 80 33 51 1443 81 51 2108 Cuts at: 4217 5929 96 Size: 1712 138 Fragments arranged by size: 2108 1712 1443 274 138 81 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 2437 1316 Size: 1121 4850 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2418 2176 Size: 242 5729 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 2951 3119 4100 2951 Size: 168 981 4822 Fragments arranged by size: 4822 981 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI