Restriction map for
pFN21AB7413
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AocI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BcgI BciVI BfaI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI HincII HindII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 236 GTCTAC GT^[AC][GT]AC
AcyI 696 GGCGCC G[GA]^CG[CT]C
AhdI 793 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 551 GTGCAC G^TGCAC
ApaI 941 GGGCCC GGGCC^C
ApaLI 551 GTGCAC G^TGCAC
Asp700I 115 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 793 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 127 TGGCCA TGG^CCA
BbeI 696 GGCGCC GGCGC^C
BbrPI 496 CACGTG CAC^GTG
BbvCI 908 CCTCAGC CCTCAGC(-5/-2)
BclI 467 TGATCA T^GATCA
BglI 620 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 496 CACGTG [CT]AC^GT[GA]
BsaHI 696 GGCGCC G[GA]^CG[CT]C
BseRI 315 GAGGAG GAGGAG(10/8)
BsmI 135 GAATGC GAATGC(1/-1)
Bsp1407I 363 TGTACA T^GTACA
BspMI 653 ACCTGC ACCTGC(4/8)
BsrGI 363 TGTACA T^GTACA
BssSI 776 CTCGTG CACGAG(-5/-1)
BstXI 651 CCACCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 686 CCGTGG C^C[GA][CT]GG
EaeI 127 TGGCCA [CT]^GGCC[GA]
Eam1105I 793 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 130 CCAAGG C^C[AT][AT]GG
FbaI 467 TGATCA T^GATCA
Hin1I 696 GGCGCC G[GA]^CG[CT]C
HindIII 977 AAGCTT A^AGCTT
KasI 696 GGCGCC G^GCGCC
MaeIII 871 GTCAC ^GT[ACGT]AC
MluNI 127 TGGCCA TGG^CCA
MscI 127 TGGCCA TGG^CCA
NarI 696 GGCGCC GG^CGCC
PflMI 751 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 496 CACGTG CAC^GTG
PmlI 496 CACGTG CAC^GTG
PpuMI 743 AGGACCT [GA]G^G[AT]CC[CT]
SacI 881 GAGCTC GAGCT^C
SfcI 247 CTACAG C^T[GA][CT]AG
SfoI 696 GGCGCC GGC^GCC
SspBI 363 TGTACA T^GTACA
StyI 130 CCAAGG C^C[AT][AT]GG
Tsp45I 871 GTCAC ^GT[GC]AC
Tsp509I 933 AATT ^AATT
Van91I 751 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 255 CCAAATCCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 115 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 364 GTAC GT^AC
1114 GTAC
AlwI 384 GATCC GGATC(4/5)
102 GGATC
AspHI 551 GTGCAC G[AT]GC[AT]^C
881 GAGCTC
AvaI 594 CCCGGG C^[CT]CG[GA]G
875 CCCGGG
BbsI 451 GAAGAC GAAGAC(2/6)
1108 GAAGAC
BlpI 286 GCTCAGC GC^T[ACGT]AGC
582 GCTGAGC
Bpu1102I 286 GCTCAGC GC^T[ACGT]AGC
582 GCTGAGC
BpuAI 451 GAAGAC GAAGAC(2/6)
1108 GAAGAC
BsgI 182 CTGCAC GTGCAG(16/14)
310 GTGCAG
BsiHKAI 551 GTGCAC G[AT]GC[AT]^C
881 GAGCTC
BsmFI 436 GGGAC GGGAC(10/14)
1140 GTCCC
BsoBI 594 CCCGGG C^[CT]CG[GA]G
875 CCCGGG
BsrI 59 ACTGG ACTGG(1/-1)
1125 ACTGG
BstYI 102 GGATCT [GA]^GATC[CT]
383 AGATCC
CelII 286 GCTCAGC GC^T[ACGT]AGC
582 GCTGAGC
CfoI 537 GCGC GCG^C
697 GCGC
EcoNI 1047 CCTGACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1071 CCTCATTCAGG
HaeII 536 AGCGCC [GA]GCGC^[CT]
696 GGCGCC
HhaI 537 GCGC GCG^C
697 GCGC
HinP1I 537 GCGC G^CGC
697 GCGC
MflI 102 GGATCT [GA]^GATC[CT]
383 AGATCC
MslI 456 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
652 CACCTGCATG
PleI 438 GACTC GAGTC(4/5)
799 GAGTC
PvuII 289 CAGCTG CAG^CTG
1058 CAGCTG
RsaI 364 GTAC GT^AC
1114 GTAC
SapI 1147 GAAGAGC GCTCTTC(1/4)
4 GCTCTTC
SmaI 594 CCCGGG CCC^GGG
875 CCCGGG
TfiI 16 GATTC G^A[AT]TC
120 GATTC
XhoII 102 GGATCT [GA]^GATC[CT]
383 AGATCC
XmaCI 594 CCCGGG C^CCGGG
875 CCCGGG
XmaI 594 CCCGGG C^CCGGG
875 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI TspRI