(Circular) (Six-base) MAPSORT of: pFN21AB7413.seq Check: 1730 from: 1 to: 5860 pFN21AB7413 5860 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2667 2667 Size: 5860 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2242 3203 1937 Size: 305 961 4594 Fragments arranged by size: 4594 961 305 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3028 3923 5307 5575 1632 Size: 1396 895 1384 268 1917 Fragments arranged by size: 1917 1396 1384 895 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 2803 4589 2803 Size: 1786 4074 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 2545 2762 2795 3068 3603 5175 2545 Size: 217 33 273 535 1572 3230 Fragments arranged by size: 3230 1572 535 273 217 33 ApaI G_GGCC'C Cuts at: 2950 2950 Size: 5860 ApaLI G'TGCA_C Cuts at: 2556 3917 5073 2556 Size: 1361 1156 3343 Fragments arranged by size: 3343 1361 1156 ApoI r'AATT_y Cuts at: 1493 3169 3395 3547 5656 1493 Size: 1676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1676 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2599 2880 3185 1870 Size: 73 656 281 305 4545 Fragments arranged by size: 4545 656 305 281 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2512 2701 3015 3181 4636 Size: 325 217 627 725 189 314 166 1455 Cuts at: 4636 618 Size: 1842 Fragments arranged by size: 1842 1455 725 627 325 314 217 189 166 BanII G_rGCy'C Cuts at: 1391 2715 2890 2950 3179 1391 Size: 1324 175 60 229 4072 Fragments arranged by size: 4072 1324 229 175 60 BbeI G_GCGC'C Cuts at: 2705 2705 Size: 5860 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2463 3120 928 Size: 971 564 657 3668 Fragments arranged by size: 3668 971 657 564 BbvCI CC'TCA_GC Cuts at: 2914 2914 Size: 5860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 2472 1548 Size: 924 4936 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2631 4471 136 Size: 107 122 71 631 605 959 1840 1525 Fragments arranged by size: 1840 1525 959 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 2292 2588 3277 2292 Size: 296 689 4875 Fragments arranged by size: 4875 689 296 Bme1580I G_kGCm'C Cuts at: 1790 2560 2950 3018 3921 5077 1790 Size: 770 390 68 903 1156 2573 Fragments arranged by size: 2573 1156 903 770 390 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2074 3591 4549 Size: 416 53 83 399 24 657 1517 958 Cuts at: 4549 442 Size: 1753 Fragments arranged by size: 1753 1517 958 657 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2423 2423 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2510 2729 2745 4520 1239 Size: 1271 219 16 1775 2579 Fragments arranged by size: 2579 1775 1271 219 16 Bpu10I CC'TnA_GC Cuts at: 2517 2914 2517 Size: 397 5463 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2503 3651 493 Size: 711 1299 1148 2702 Fragments arranged by size: 2702 1299 1148 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2702 3667 4049 4684 Size: 53 83 186 936 1169 965 382 635 Cuts at: 4684 275 Size: 1451 Fragments arranged by size: 1451 1169 965 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4293 4691 4965 5112 5523 4293 Size: 398 274 147 411 4630 Fragments arranged by size: 4630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2335 1186 Size: 1149 4711 BseYI C'CCAG_C Cuts at: 2221 2538 2755 3011 5063 5559 2221 Size: 317 217 256 2052 496 2522 Fragments arranged by size: 2522 2052 496 317 256 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2172 2336 1163 Size: 573 436 164 4687 Fragments arranged by size: 4687 573 436 164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2560 2890 3921 4006 5077 2560 Size: 330 1031 85 1071 3343 Fragments arranged by size: 3343 1071 1031 330 85 BsmI GAATG_Cn' Cuts at: 2146 3371 3464 2146 Size: 1225 93 4542 Fragments arranged by size: 4542 1225 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2560 2715 2890 2950 3018 3179 3921 Size: 399 770 155 175 60 68 161 742 Cuts at: 3921 4006 5077 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 770 742 399 175 161 155 85 68 60 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2667 3215 844 Size: 1823 548 3489 Fragments arranged by size: 3489 1823 548 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4504 4691 1700 Size: 110 2694 187 2869 Fragments arranged by size: 2869 2694 187 110 BsrGI T'GTAC_A Cuts at: 96 2368 96 Size: 2272 3588 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 2781 3920 4932 2781 Size: 1139 1012 3709 Fragments arranged by size: 3709 1139 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstXI CCAn_nnnn'nTGG Cuts at: 2663 2663 Size: 5860 BstYI r'GATC_y Cuts at: 1972 2107 2388 3190 3557 3942 3959 5411 5497 Size: 135 281 802 367 385 17 1452 86 Cuts at: 5497 5509 5855 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 802 385 367 346 281 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2691 513 Size: 552 939 687 3682 Fragments arranged by size: 3682 939 687 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 3376 4170 4190 3376 Size: 794 20 5046 Fragments arranged by size: 5046 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2132 3307 4196 Size: 54 1425 50 275 18 302 1175 889 Cuts at: 4196 5825 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1175 889 302 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2016 2414 3147 3790 1353 Size: 663 398 733 643 3423 Fragments arranged by size: 3423 733 663 643 398 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2880 4447 4821 4967 387 Size: 1064 138 1291 1567 374 146 1280 Fragments arranged by size: 1567 1291 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2888 2888 Size: 5860 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 2729 2745 3028 3923 4520 5307 Size: 393 878 219 16 283 895 597 787 Cuts at: 5307 5575 1239 Size: 268 1524 Fragments arranged by size: 1524 895 878 787 597 393 283 268 219 16 EcoNI CCTnn'n_nnAGG Cuts at: 3056 3080 3056 Size: 24 5836 EcoO109I rG'GnC_Cy Cuts at: 2325 2749 2792 2325 Size: 424 43 5393 Fragments arranged by size: 5393 424 43 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2977 2977 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2545 2705 5007 1678 Size: 867 160 2302 2531 Fragments arranged by size: 2531 2302 867 160 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2789 2821 4501 4533 4575 Size: 32 135 32 1217 32 1680 32 42 Cuts at: 4575 4607 1373 Size: 32 2626 Fragments arranged by size: 2626 1680 1217 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 2982 748 Size: 2234 3626 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KasI G'GCGC_C Cuts at: 2701 2701 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2134 10 Size: 54 1425 343 302 3736 Fragments arranged by size: 3736 1425 343 302 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 2661 3818 4177 4336 518 Size: 648 1299 196 1157 359 159 2042 Fragments arranged by size: 2042 1299 1157 648 359 196 159 MspA1I CmG'CkG Cuts at: 1606 2212 2296 3065 3276 3953 5101 5346 1606 Size: 606 84 769 211 677 1148 245 2120 Fragments arranged by size: 2120 1148 769 677 606 245 211 84 NarI GG'CG_CC Cuts at: 2702 2702 Size: 5860 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4369 Fragments arranged by size: 4369 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 3218 4763 1132 Size: 97 1989 1545 2229 Fragments arranged by size: 2229 1989 1545 97 PasI CC'CwG_GG Cuts at: 1348 2438 1348 Size: 1090 4770 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 2762 1108 Size: 1654 4206 PfoI T'CCnGG_A Cuts at: 2745 2745 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PmlI CAC'GTG Cuts at: 2503 2503 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2749 2749 Size: 5860 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2946 2946 Size: 5860 PstI C_TGCA'G Cuts at: 830 3212 830 Size: 2382 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2296 3065 2296 Size: 769 5091 SacI G_AGCT'C Cuts at: 2890 2890 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SapI GCTCTTCn'nnn_ Cuts at: 2016 3147 2016 Size: 1131 4729 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2252 3208 4343 5024 5215 826 Size: 219 1207 956 1135 681 191 1471 Fragments arranged by size: 1471 1207 1135 956 681 219 191 SfoI GGC'GCC Cuts at: 2703 2703 Size: 5860 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 2601 2882 3187 1872 Size: 729 281 305 4545 Fragments arranged by size: 4545 729 305 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2004 2135 3299 513 Size: 552 939 131 1164 3074 Fragments arranged by size: 3074 1164 939 552 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3887 3904 4057 4242 4687 325 Size: 1255 2307 17 153 185 445 1498 Fragments arranged by size: 2307 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2368 4106 5818 Size: 274 80 33 51 1443 391 1738 1712 Cuts at: 5818 96 Size: 138 Fragments arranged by size: 1738 1712 1443 391 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2136 2168 5820 5852 2136 Size: 32 3652 32 2144 Fragments arranged by size: 3652 2144 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2267 2267 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 2599 2880 3185 1870 Size: 729 281 305 4545 Fragments arranged by size: 4545 729 305 281 XmnI GAAnn'nnTTC Cuts at: 2124 3989 2124 Size: 1865 3995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI