(Circular) (Six-base) MAPSORT of: pFN21AB7412.seq Check: 2732 from: 1 to: 5722 pFN21AB7412 5722 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2850 3532 278 Size: 53 83 186 936 1314 682 2468 Fragments arranged by size: 2468 1314 936 682 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2382 2883 3000 3785 5169 5437 1632 Size: 750 501 117 785 1384 268 1917 Fragments arranged by size: 1917 1384 785 750 501 268 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 4621 5598 1203 Size: 3418 977 1327 Fragments arranged by size: 3418 1327 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 2034 1166 Size: 868 4854 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5722 AlwNI CAG_nnn'CTG Cuts at: 3465 5037 3465 Size: 1572 4150 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 2128 2697 2731 2884 2947 3031 3257 3409 Size: 635 569 34 153 63 84 226 152 Cuts at: 3409 5518 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 635 569 226 153 152 84 63 34 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 3047 1870 Size: 73 1104 4545 Fragments arranged by size: 4545 1104 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2749 2782 2749 Size: 33 5689 BamHI G'GATC_C Cuts at: 2791 3052 3419 2791 Size: 261 367 5094 Fragments arranged by size: 5094 367 261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2147 2293 2475 2929 2960 Size: 325 217 627 360 146 182 454 31 Cuts at: 2960 3043 4498 618 Size: 83 1455 1842 Fragments arranged by size: 1842 1455 627 454 360 325 217 182 146 83 31 BanII G_rGCy'C Cuts at: 1391 2368 3041 1391 Size: 977 673 4072 Fragments arranged by size: 4072 977 673 BbeI G_GCGC'C Cuts at: 2151 2297 2479 2151 Size: 146 182 5394 Fragments arranged by size: 5394 182 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2774 928 Size: 971 875 3876 Fragments arranged by size: 3876 971 875 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3913 3947 1903 Size: 34 50 34 1892 34 3678 Fragments arranged by size: 3678 1892 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2777 3614 4824 2777 Size: 837 1210 3675 Fragments arranged by size: 3675 1210 837 BclI T'GATC_A Cuts at: 1548 1548 Size: 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4333 136 Size: 107 122 71 631 605 2661 1525 Fragments arranged by size: 2661 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 2264 2932 3783 4939 1790 Size: 474 668 851 1156 2573 Fragments arranged by size: 2573 1156 851 668 474 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 2237 3021 Size: 416 53 83 399 24 636 184 784 Cuts at: 3021 3453 4411 442 Size: 432 958 1753 Fragments arranged by size: 1753 958 784 636 432 416 399 184 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2241 1056 Size: 1185 4537 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2619 2619 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 2874 4382 1239 Size: 1388 247 1508 2579 Fragments arranged by size: 2579 1508 1388 247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2848 3847 4712 5010 2848 Size: 999 865 298 3560 Fragments arranged by size: 3560 999 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4385 882 Size: 617 2886 2219 Fragments arranged by size: 2886 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 5716 5716 Size: 5722 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2294 2476 2847 Size: 53 83 186 936 615 146 182 371 Cuts at: 2847 3529 3911 4546 275 Size: 682 382 635 1451 Fragments arranged by size: 1451 936 682 635 615 382 371 186 182 146 83 53 BsaWI w'CCGG_w Cuts at: 4155 4553 4827 4974 5385 4155 Size: 398 274 147 411 4492 Fragments arranged by size: 4492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2600 2630 1178 Size: 30 165 30 1197 30 4270 Fragments arranged by size: 4270 1197 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 1186 Size: 874 4848 BseYI C'CCAG_C Cuts at: 2012 4925 5421 2012 Size: 2913 496 2313 Fragments arranged by size: 2913 2313 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 2196 3172 3933 4961 1540 Size: 275 186 21 174 976 761 1028 2301 Fragments arranged by size: 2301 1028 976 761 275 186 174 21 BsiHKAI G_wGCw'C Cuts at: 2328 2435 3783 3868 4939 2328 Size: 107 1348 85 1071 3111 Fragments arranged by size: 3111 1348 1071 107 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2328 2368 2435 2932 3041 3783 Size: 399 474 64 40 67 497 109 742 Cuts at: 3783 3868 4939 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 497 474 399 109 85 67 64 40 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 3611 5341 3611 Size: 1730 3992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 844 Size: 2233 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3609 1394 Size: 429 1786 3507 Fragments arranged by size: 3507 1786 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4217 4391 65 Size: 1183 2969 174 1396 Fragments arranged by size: 2969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 4366 4553 1700 Size: 110 223 2333 187 2869 Fragments arranged by size: 2869 2333 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 2214 1925 Size: 289 5433 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5722 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstXI CCAn_nnnn'nTGG Cuts at: 2671 2671 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2791 3052 3419 3804 3821 5273 5359 5371 Size: 819 261 367 385 17 1452 86 12 Cuts at: 5371 5717 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 819 385 367 346 261 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2208 513 Size: 552 1143 4027 Fragments arranged by size: 4027 1143 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 2490 2951 3238 4032 4052 2490 Size: 461 287 794 20 4160 Fragments arranged by size: 4160 794 461 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 3169 4058 Size: 54 1425 50 275 18 372 967 889 Cuts at: 4058 5687 8 Size: 1629 43 Fragments arranged by size: 1629 1425 967 889 372 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2502 2566 3652 1353 Size: 1149 64 1086 3423 Fragments arranged by size: 3423 1149 1086 64 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2743 4309 4683 4829 387 Size: 1064 138 1154 1566 374 146 1280 Fragments arranged by size: 1566 1280 1154 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2382 2627 2874 2883 3000 3785 4382 Size: 393 750 245 247 9 117 785 597 Cuts at: 4382 5169 5437 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 785 750 597 393 268 247 245 117 9 EcoNI CCTnn'n_nnAGG Cuts at: 2531 2531 Size: 5722 EcoO109I rG'GnC_Cy Cuts at: 2466 2466 Size: 5722 EcoRI G'AATT_C Cuts at: 2731 2884 3031 3409 2731 Size: 153 147 378 5044 Fragments arranged by size: 5044 378 153 147 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2798 2798 Size: 5722 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 2151 2297 2405 2479 4869 1678 Size: 473 146 108 74 2390 2531 Fragments arranged by size: 2531 2390 473 146 108 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2783 2815 4363 4395 4437 Size: 32 135 32 1211 32 1548 32 42 Cuts at: 4437 4469 1373 Size: 32 2626 Fragments arranged by size: 2626 1548 1211 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3066 3312 669 Size: 988 281 1128 246 3079 Fragments arranged by size: 3079 1128 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5722 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KasI G'GCGC_C Cuts at: 2147 2293 2475 2147 Size: 146 182 5394 Fragments arranged by size: 5394 182 146 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2781 4836 5020 1305 Size: 1476 2055 184 2007 Fragments arranged by size: 2055 2007 1476 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2204 10 Size: 54 1425 343 372 3528 Fragments arranged by size: 3528 1425 372 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2034 3680 4039 4198 518 Size: 648 868 1646 359 159 2042 Fragments arranged by size: 2042 1646 868 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2210 3138 3815 4963 5208 1606 Size: 478 126 928 677 1148 245 2120 Fragments arranged by size: 2120 1148 928 677 478 245 126 NarI GG'CG_CC Cuts at: 2148 2294 2476 2148 Size: 146 182 5394 Fragments arranged by size: 5394 182 146 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5170 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NheI G'CTAG_C Cuts at: 1052 2237 1052 Size: 1185 4537 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 2518 3080 4625 1132 Size: 97 1289 562 1545 2229 Fragments arranged by size: 2229 1545 1289 562 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5722 PfoI T'CCnGG_A Cuts at: 2913 2913 Size: 5722 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2466 2466 Size: 5722 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PstI C_TGCA'G Cuts at: 830 2169 3074 830 Size: 1339 905 3478 Fragments arranged by size: 3478 1339 905 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 SacII CC_GC'GG Cuts at: 2211 2211 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SapI GCTCTTCn'nnn_ Cuts at: 2566 2566 Size: 5722 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 2165 3070 4205 4886 5077 826 Size: 219 1120 905 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 905 681 219 191 SfoI GGC'GCC Cuts at: 2149 2295 2477 2149 Size: 146 182 5394 Fragments arranged by size: 5394 182 146 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 2033 1700 Size: 333 5389 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2863 3826 4727 4989 820 Size: 197 926 920 963 901 262 1553 Fragments arranged by size: 1553 963 926 920 901 262 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 2518 3080 1132 Size: 1386 562 3774 Fragments arranged by size: 3774 1386 562 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5722 StyI C'CwwG_G Cuts at: 513 1065 3161 513 Size: 552 2096 3074 Fragments arranged by size: 3074 2096 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2391 2664 3749 3766 3919 4104 4549 Size: 1255 811 273 1085 17 153 185 445 Cuts at: 4549 325 Size: 1498 Fragments arranged by size: 1498 1255 1085 811 445 273 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3004 3968 5680 Size: 274 80 33 51 1443 1027 964 1712 Cuts at: 5680 96 Size: 138 Fragments arranged by size: 1712 1443 1027 964 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2482 2514 5682 5714 2482 Size: 32 3168 32 2490 Fragments arranged by size: 3168 2490 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5722 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 3851 3851 Size: 5722 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2848 3530 276 Size: 53 83 186 936 1314 682 2468 Fragments arranged by size: 2468 1314 936 682 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI