Restriction map for
pFN21AB7409
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI Eco47III EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MseI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 345 GTCTAC GT^[AC][GT]AC
AfaI 295 GTAC GT^AC
AhdI 337 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 488 CCTCAGG CC^T[ACGT]AGG
AspEI 337 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 555 GTGCTC G[AT]GC[AT]^C
AvaI 58 CCCGGG C^[CT]CG[GA]G
AvrII 178 CCTAGG C^CTAGG
BalI 299 TGGCCA TGG^CCA
BamHI 125 GGATCC G^GATCC
BbvI 480 GCAGC GCAGC(8/12)
BfaI 179 CTAG C^TAG
BglI 25 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 178 CCTAGG C^CTAGG
BlpI 145 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 145 GCTGAGC GC^T[ACGT]AGC
BsaI 458 GAGACC GGTCTC(1/5)
BsiEI 17 CGGCCG CG[GA][CT]^CG
BsiHKAI 555 GTGCTC G[AT]GC[AT]^C
BsmFI 62 GGGAC GGGAC(10/14)
BsoBI 58 CCCGGG C^[CT]CG[GA]G
BspMI 272 ACCTGC ACCTGC(4/8)
BsrBI 520 CCGCTC CCGCTC(-3/-3)
BstEII 499 GGTGACC G^GT[ACGT]ACC
BstPI 499 GGTGACC G^GT[ACGT]ACC
BstXI 129 CCAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 125 GGATCC [GA]^GATC[CT]
Bsu36I 488 CCTCAGG CC^T[ACGT]AGG
CelII 145 GCTGAGC GC^T[ACGT]AGC
DraII 384 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 112 CCGTGG C^C[GA][CT]GG
EagI 17 CGGCCG C^GGCCG
Eam1105I 337 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 370 GAAGAG CTCTTC(1/4)
EclXI 17 CGGCCG C^GGCCG
Eco52I 17 CGGCCG C^GGCCG
Eco81I 488 CCTCAGG CC^T[ACGT]AGG
EcoO109I 384 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 499 GGTGACC G^GT[ACGT]ACC
HphI 499 GGTGA GGTGA(8/7)
Ksp632I 370 GAAGAG CTCTTC(1/4)
MaeI 179 CTAG C^TAG
MflI 125 GGATCC [GA]^GATC[CT]
MluNI 299 TGGCCA TGG^CCA
MscI 299 TGGCCA TGG^CCA
NlaIII 252 CATG CATG^
PflMI 411 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 384 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 295 GTAC GT^AC
SapI 370 GAAGAGC GCTCTTC(1/4)
ScaI 294 AGTACT AGT^ACT
SmaI 58 CCCGGG CCC^GGG
StuI 76 AGGCCT AGG^CCT
TseI 480 GCAGC G^C[AT]GC
Van91I 411 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 189 CCAACACACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 125 GGATCC [GA]^GATC[CT]
XmaCI 58 CCCGGG C^CCGGG
XmaI 58 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AluI 149 AGCT AG^CT
366 AGCT
AvaII 385 GGACC G^G[AT]CC
517 GGTCC
BpmI 72 CTGGAG CTGGAG(16/14)
137 CTGGAG
BseRI 537 CTCCTC GAGGAG(10/8)
558 CTCCTC
BsmAI 458 GAGAC GTCTC(1/5)
535 GTCTC
BstF5I 229 CATCC GGATG(2/0)
563 CATCC
Cac8I 92 GCGGGC GC[ACGT]^[ACGT]GC
315 GCAAGC
EaeI 17 CGGCCG [CT]^GGCC[GA]
299 TGGCCA
FokI 229 CATCC GGATG(9/13)
563 CATCC
HgaI 64 GACGC GACGC(5/10)
261 GCGTC
MaeIII 50 GTCAC ^GT[ACGT]AC
500 GTGAC
MboII 370 GAAGA GAAGA(8/7)
402 TCTTC
SmlI 527 CTCAAG C^T[CT][GA]AG
540 CTCAAG
Tsp45I 50 GTCAC ^GT[GC]AC
500 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AlwI BanII BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI BstUI CfoI
Cfr13I DdeI DpnII EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI TspRI