(Circular) (Six-base) MAPSORT of: pFN21AB7409.seq Check: 18 from: 1 to: 5275 pFN21AB7409 5275 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3085 278 Size: 53 83 186 936 1549 2468 Fragments arranged by size: 2468 1549 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2351 2618 1937 Size: 414 267 4594 Fragments arranged by size: 4594 414 267 Acc65I G'GTAC_C Cuts at: 2596 2596 Size: 5275 AclI AA'CG_TT Cuts at: 1563 3402 3775 1563 Size: 1839 373 3063 Fragments arranged by size: 3063 1839 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2394 3338 4722 4990 1632 Size: 762 944 1384 268 1917 Fragments arranged by size: 1917 1384 944 762 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5078 AflIII A'CryG_T Cuts at: 1203 4174 5151 1203 Size: 2971 977 1327 Fragments arranged by size: 2971 1327 977 AgeI A'CCGG_T Cuts at: 4106 4106 Size: 5275 AhdI GACnn_n'nnGTC Cuts at: 2347 4004 2347 Size: 1657 3618 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5275 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5275 AlwNI CAG_nnn'CTG Cuts at: 3018 4590 3018 Size: 1572 3703 ApaLI G'TGCA_C Cuts at: 3332 4488 3332 Size: 1156 4119 ApoI r'AATT_y Cuts at: 1493 2584 2810 2962 5071 1493 Size: 1091 226 152 2109 1697 Fragments arranged by size: 2109 1697 1091 226 152 AseI AT'TA_AT Cuts at: 160 1032 3829 160 Size: 872 2797 1606 Fragments arranged by size: 2797 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5275 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2600 1870 Size: 73 120 537 4545 Fragments arranged by size: 4545 537 120 73 AvrII C'CTAG_G Cuts at: 2183 2183 Size: 5275 BamHI G'GATC_C Cuts at: 2130 2605 2972 2130 Size: 475 367 4433 Fragments arranged by size: 4433 475 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2596 4051 618 Size: 325 217 627 809 1455 1842 Fragments arranged by size: 1842 1455 809 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2032 2111 2594 1391 Size: 622 19 79 483 4072 Fragments arranged by size: 4072 622 483 79 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3466 3500 1903 Size: 34 1529 34 3678 Fragments arranged by size: 3678 1529 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3167 4377 3167 Size: 1210 4065 BclI T'GATC_A Cuts at: 1548 1548 Size: 5275 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 3886 136 Size: 107 122 71 631 605 364 1850 1525 Fragments arranged by size: 1850 1525 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 5270 5270 Size: 5275 BlpI GC'TnA_GC Cuts at: 2151 2692 2151 Size: 541 4734 Bme1580I G_kGCm'C Cuts at: 1790 3336 4492 1790 Size: 1546 1156 2573 Fragments arranged by size: 2573 1546 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5275 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2309 2521 3006 Size: 416 53 83 399 24 892 212 485 Cuts at: 3006 3964 442 Size: 958 1753 Fragments arranged by size: 1753 958 892 485 416 399 212 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5275 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2163 3935 1239 Size: 859 65 1772 2579 Fragments arranged by size: 2579 1772 859 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2517 2530 3400 4265 4563 2517 Size: 13 870 865 298 3229 Fragments arranged by size: 3229 870 865 298 13 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2457 3938 882 Size: 617 958 1481 2219 Fragments arranged by size: 2219 1481 958 617 BsaAI yAC'GTr Cuts at: 493 1204 3066 493 Size: 711 1862 2702 Fragments arranged by size: 2702 1862 711 BsaBI GATnn'nnATC Cuts at: 5269 5269 Size: 5275 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3082 3464 4099 275 Size: 53 83 186 936 1549 382 635 1451 Fragments arranged by size: 1549 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3708 4106 4380 4527 4938 3708 Size: 398 274 147 411 4045 Fragments arranged by size: 4045 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5050 Fragments arranged by size: 5050 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2533 2554 1186 Size: 1347 21 3907 Fragments arranged by size: 3907 1347 21 BseYI C'CCAG_C Cuts at: 4478 4974 4478 Size: 496 4779 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4702 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 2725 3486 4514 1540 Size: 275 186 24 700 761 1028 2301 Fragments arranged by size: 2301 1028 761 700 275 186 24 BsiHKAI G_wGCw'C Cuts at: 2564 3336 3421 4492 2564 Size: 772 85 1071 3347 Fragments arranged by size: 3347 1071 772 85 BsmI GAATG_Cn' Cuts at: 2786 2879 2786 Size: 93 5182 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2032 2111 2564 2594 3336 3421 Size: 399 223 19 79 453 30 742 85 Cuts at: 3421 4492 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 453 399 223 85 79 30 19 BspEI T'CCGG_A Cuts at: 4938 4938 Size: 5275 BspHI T'CATG_A Cuts at: 3164 4894 3164 Size: 1730 3545 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2286 2630 844 Size: 1442 344 3489 Fragments arranged by size: 3489 1442 344 BsrBI CCG'CTC Cuts at: 1394 1823 2527 3162 1394 Size: 429 704 635 3507 Fragments arranged by size: 3507 704 635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3770 3944 65 Size: 1183 2522 174 1396 Fragments arranged by size: 2522 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3919 4106 1700 Size: 110 2109 187 2869 Fragments arranged by size: 2869 2109 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5275 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5275 BssSI C'ACGA_G Cuts at: 3335 4347 3335 Size: 1012 4263 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5275 BstBI TT'CG_AA Cuts at: 4079 4079 Size: 5275 BstEII G'GTnAC_C Cuts at: 1184 2504 1184 Size: 1320 3955 BstXI CCAn_nnnn'nTGG Cuts at: 2141 2141 Size: 5275 BstYI r'GATC_y Cuts at: 1972 2130 2605 2972 3357 3374 4826 4912 4924 Size: 158 475 367 385 17 1452 86 12 Cuts at: 4924 5270 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 475 385 367 346 158 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2494 2494 Size: 5275 BtgI C'CryG_G Cuts at: 513 1065 2004 2117 513 Size: 552 939 113 3671 Fragments arranged by size: 3671 939 552 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4536 Fragments arranged by size: 4536 683 56 BtsI GCAGTG_nn' Cuts at: 2056 2791 3585 3605 2056 Size: 735 794 20 3726 Fragments arranged by size: 3726 794 735 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5275 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5240 DraI TTT'AAA Cuts at: 2579 2579 Size: 5275 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4282 809 Size: 507 2966 1802 Fragments arranged by size: 2966 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2304 2722 Size: 54 1425 50 275 18 192 282 418 Cuts at: 2722 3611 5240 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 418 282 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2022 2722 1537 Size: 275 210 700 4090 Fragments arranged by size: 4090 700 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 2370 3205 1353 Size: 1017 835 3423 Fragments arranged by size: 3423 1017 835 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2076 3862 4236 4382 387 Size: 1064 138 487 1786 374 146 1280 Fragments arranged by size: 1786 1280 1064 487 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2163 2394 3338 3935 4722 4990 Size: 393 466 65 231 944 597 787 268 Cuts at: 4990 1239 Size: 1524 Fragments arranged by size: 1524 944 787 597 466 393 268 231 65 EcoO109I rG'GnC_Cy Cuts at: 2390 2390 Size: 5275 EcoRI G'AATT_C Cuts at: 2584 2962 2584 Size: 378 4897 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 5275 FspI TGC'GCA Cuts at: 3781 3781 Size: 5275 HaeII r_GCGC'y Cuts at: 1678 4422 1678 Size: 2744 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 2333 2365 3916 Size: 32 135 32 543 32 186 32 1551 Cuts at: 3916 3948 3990 4022 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1551 543 186 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2619 2865 669 Size: 988 281 681 246 3079 Fragments arranged by size: 3079 988 681 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5275 HpaI GTT'AAC Cuts at: 2865 2865 Size: 5275 KpnI G_GTAC'C Cuts at: 2600 2600 Size: 5275 MfeI C'AATT_G Cuts at: 2874 2874 Size: 5275 MluI A'CGCG_T Cuts at: 5151 5151 Size: 5275 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4389 4573 1305 Size: 3084 184 2007 Fragments arranged by size: 3084 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2306 10 Size: 54 1425 343 474 2979 Fragments arranged by size: 2979 1425 474 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3233 3592 3751 518 Size: 648 2067 359 159 2042 Fragments arranged by size: 2067 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2691 3368 4516 4761 1606 Size: 1085 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3784 Fragments arranged by size: 3784 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5275 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5275 NotI GC'GGCC_GC Cuts at: 2722 2722 Size: 5275 NspI r_CATG'y Cuts at: 1132 1229 2633 4178 1132 Size: 97 1404 1545 2229 Fragments arranged by size: 2229 1545 1404 97 PasI CC'CwG_GG Cuts at: 1348 2447 1348 Size: 1099 4176 PciI A'CATG_T Cuts at: 4174 4174 Size: 5275 PflMI CCAn_nnn'nTGG Cuts at: 1108 2422 1108 Size: 1314 3961 PmeI GTTT'AAAC Cuts at: 2579 2579 Size: 5275 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3344 3376 4857 4889 3344 Size: 32 1481 32 3730 Fragments arranged by size: 3730 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2390 2390 Size: 5275 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5275 PsiI TTA'TAA Cuts at: 2845 2845 Size: 5275 PstI C_TGCA'G Cuts at: 830 2627 830 Size: 1797 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5275 SalI G'TCGA_C Cuts at: 1936 2617 1936 Size: 681 4594 SapI GCTCTTCn'nnn_ Cuts at: 2370 2370 Size: 5275 SbfI CC_TGCA'GG Cuts at: 2627 2627 Size: 5275 ScaI AGT'ACT Cuts at: 2301 3523 5235 2301 Size: 1222 1712 2341 Fragments arranged by size: 2341 1712 1222 SfcI C'TryA_G Cuts at: 826 1045 2623 3758 4439 4630 826 Size: 219 1578 1135 681 191 1471 Fragments arranged by size: 1578 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5275 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5275 SmaI CCC'GGG Cuts at: 1872 2065 2602 1872 Size: 193 537 4545 Fragments arranged by size: 4545 537 193 SmlI C'TyrA_G Cuts at: 820 1017 1943 2532 2545 3379 4280 4542 820 Size: 197 926 589 13 834 901 262 1553 Fragments arranged by size: 1553 926 901 834 589 262 197 13 SnaBI TAC'GTA Cuts at: 493 3066 493 Size: 2573 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5275 SphI G_CATG'C Cuts at: 1132 2633 1132 Size: 1501 3774 SspI AAT'ATT Cuts at: 5 52 3199 5 Size: 47 3147 2081 Fragments arranged by size: 3147 2081 47 StuI AGG'CCT Cuts at: 1517 2083 1517 Size: 566 4709 StyI C'CwwG_G Cuts at: 513 1065 2004 2126 2183 2479 2714 513 Size: 552 939 122 57 296 235 3074 Fragments arranged by size: 3074 939 552 296 235 122 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3302 3319 3472 3657 4102 325 Size: 1255 1722 17 153 185 445 1498 Fragments arranged by size: 1722 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2299 3521 5233 Size: 274 80 33 51 1443 322 1222 1712 Cuts at: 5233 96 Size: 138 Fragments arranged by size: 1712 1443 1222 322 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5235 5267 5235 Size: 32 5243 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5275 XbaI T'CTAG_A Cuts at: 2611 2611 Size: 5275 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2201 2201 Size: 5275 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5275 XmaI C'CCGG_G Cuts at: 1870 2063 2600 1870 Size: 193 537 4545 Fragments arranged by size: 4545 537 193 XmnI GAAnn'nnTTC Cuts at: 3404 3404 Size: 5275 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3083 276 Size: 53 83 186 936 1549 2468 Fragments arranged by size: 2468 1549 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI