Restriction map for
pFN21AB7409
(Linear) (Six-base) MAPSORT of: KIBB7409.seq Check: 5008 from: 1 to: 570
KIBB7409.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AccI GT'mk_AC
Cuts at: 0 346 570
Size: 346 224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 570
Size: 389 181
AhdI GACnn_n'nnGTC
Cuts at: 0 342 570
Size: 342 228
AvaI C'yCGr_G
Cuts at: 0 58 570
Size: 58 512
AvrII C'CTAG_G
Cuts at: 0 178 570
Size: 178 392
BamHI G'GATC_C
Cuts at: 0 125 570
Size: 125 445
BanII G_rGCy'C
Cuts at: 0 8 27 106 570
Size: 8 19 79 464
Fragments arranged by size:
464 79 19 8
BglI GCCn_nnn'nGGC
Cuts at: 0 31 570
Size: 31 539
BlpI GC'TnA_GC
Cuts at: 0 146 570
Size: 146 424
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 516 570
Size: 304 212 54
Fragments arranged by size:
304 212 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 158 570
Size: 93 65 412
Fragments arranged by size:
412 93 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 525 570
Size: 512 13 45
Fragments arranged by size:
512 45 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 452 570
Size: 452 118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 528 549 570
Size: 528 21 21
Fragments arranged by size:
528 21 21
BsiEI CG_ry'CG
Cuts at: 0 20 570
Size: 20 550
BsiHKAI G_wGCw'C
Cuts at: 0 559 570
Size: 559 11
Bsp1286I G_dGCh'C
Cuts at: 0 8 27 106 559 570
Size: 8 19 79 453 11
Fragments arranged by size:
453 79 19 11 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 281 570
Size: 281 289
BsrBI CCG'CTC
Cuts at: 0 522 570
Size: 522 48
BstEII G'GTnAC_C
Cuts at: 0 499 570
Size: 499 71
BstXI CCAn_nnnn'nTGG
Cuts at: 0 136 570
Size: 136 434
BstYI r'GATC_y
Cuts at: 0 125 570
Size: 125 445
Bsu36I CC'TnA_GG
Cuts at: 0 489 570
Size: 489 81
BtgI C'CryG_G
Cuts at: 0 112 570
Size: 112 458
BtsI GCAGTG_nn'
Cuts at: 0 51 570
Size: 51 519
EaeI y'GGCC_r
Cuts at: 0 17 299 570
Size: 17 282 271
Fragments arranged by size:
282 271 17
EagI C'GGCC_G
Cuts at: 0 17 570
Size: 17 553
EarI CTCTTCn'nnn_
Cuts at: 0 365 570
Size: 365 205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 71 570
Size: 71 499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 158 389 570
Size: 93 65 231 181
Fragments arranged by size:
231 181 93 65
EcoO109I rG'GnC_Cy
Cuts at: 0 385 570
Size: 385 185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 231 570
Size: 231 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 328 360 570
Size: 110 32 186 32 210
Fragments arranged by size:
210 186 110 32 32
MscI TGG'CCA
Cuts at: 0 301 570
Size: 301 269
PasI CC'CwG_GG
Cuts at: 0 442 570
Size: 442 128
PflMI CCAn_nnn'nTGG
Cuts at: 0 417 570
Size: 417 153
PpuMI rG'GwC_Cy
Cuts at: 0 385 570
Size: 385 185
SapI GCTCTTCn'nnn_
Cuts at: 0 365 570
Size: 365 205
ScaI AGT'ACT
Cuts at: 0 296 570
Size: 296 274
SmaI CCC'GGG
Cuts at: 0 60 570
Size: 60 510
SmlI C'TyrA_G
Cuts at: 0 527 540 570
Size: 527 13 30
Fragments arranged by size:
527 30 13
StuI AGG'CCT
Cuts at: 0 78 570
Size: 78 492
StyI C'CwwG_G
Cuts at: 0 121 178 474 570
Size: 121 57 296 96
Fragments arranged by size:
296 121 96 57
TatI w'GTAC_w
Cuts at: 0 294 570
Size: 294 276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 196 570
Size: 196 374
XmaI C'CCGG_G
Cuts at: 0 58 570
Size: 58 512
Enzymes that do cut:
AccI AcuI AhdI AvaI AvrII BamHI BanII BglI
BlpI BmrI BpmI BpuEI BsaI BseRI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BstEII BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EciI Eco57MI EcoO109I FalI
Hin4I MscI PasI PflMI PpuMI SapI ScaI SmaI
SmlI StuI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI BaeI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII Bme1580I BmgBI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XhoI XmnI ZraI