Restriction map for
pFN21AB7408
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I AspEI AspHI
AvaI AvaII AviII AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I CelII
Cfr13I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HgaI Hin1I HindIII HpaI
HphI KasI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI Tsp45I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 395 GGTACC G^GTACC
AseI 786 ATTAAT AT^TAAT
AsnI 786 ATTAAT AT^TAAT
Asp718I 395 GGTACC G^GTACC
AspI 584 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 791 TGGCCA TGG^CCA
BanII 388 GAGCCC G[GA]GC[CT]^C
BsgI 757 CTGCAC GTGCAG(16/14)
Bsp1407I 867 TGTACA T^GTACA
BsrFI 24 GCCGGC [GA]^CCGG[CT]
BsrGI 867 TGTACA T^GTACA
BstXI 399 CCACGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 24 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
DraI 921 TTTAAA TTT^AAA
DsaI 405 CCGTGG C^C[GA][CT]GG
EcoRI 552 GAATTC G^AATTC
EcoRV 820 GATATC GAT^ATC
HapII 25 CCGG C^CGG
HincII 590 GTCAAC GT[CT]^[GA]AC
HindII 590 GTCAAC GT[CT]^[GA]AC
HpaII 25 CCGG C^CGG
KpnI 395 GGTACC GGTAC^C
MluNI 791 TGGCCA TGG^CCA
MscI 791 TGGCCA TGG^CCA
MspI 25 CCGG C^CGG
NaeI 24 GCCGGC GCC^GGC
NgoMIV 24 GCCGGC G^CCGGC
PflFI 584 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 786 ATTAAT AT^TAAT
SfaNI 1122 GATGC GCATC(5/9)
SspBI 867 TGTACA T^GTACA
SwaI 920 ATTTAAAT ATTT^AAAT
TaqI 824 TCGA T^CGA
Tth111I 584 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 824 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 113 GCGG CCGC(-3/-1)
335 GCGG
BanI 191 GGTGCC G^G[CT][GA]CC
395 GGTACC
BbsI 162 GAAGAC GAAGAC(2/6)
273 GAAGAC
BcgI 147 CGACGACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
258 CGACGACTCTGC
BfaI 1070 CTAG C^TAG
1172 CTAG
BglII 414 AGATCT A^GATCT
1032 AGATCT
BmyI 129 GGGCAC G[AGT]GC[ACT]^C
388 GAGCCC
BpmI 137 CTGGAG CTGGAG(16/14)
248 CTGGAG
BpuAI 162 GAAGAC GAAGAC(2/6)
273 GAAGAC
BsaJI 391 CCCAGG C^C[ACGT][ACGT]GG
405 CCGTGG
BsmFI 485 GGGAC GGGAC(10/14)
454 GTCCC
Bsp1286I 129 GGGCAC G[AGT]GC[ACT]^C
388 GAGCCC
BsrBI 111 GAGCGG CCGCTC(-3/-3)
333 GAGCGG
BsrI 930 ACTGG ACTGG(1/-1)
1092 CCAGT
EaeI 791 TGGCCA [CT]^GGCC[GA]
1049 CGGCCA
EarI 33 CTCTTC CTCTTC(1/4)
1134 CTCTTC
Ksp632I 33 CTCTTC CTCTTC(1/4)
1134 CTCTTC
MaeI 1070 CTAG C^TAG
1172 CTAG
MaeIII 858 GTTAC ^GT[ACGT]AC
1095 GTTAC
PleI 151 GACTC GAGTC(4/5)
262 GACTC
PstI 213 CTGCAG CTGCA^G
324 CTGCAG
SspI 727 AATATT AAT^ATT
1012 AATATT
TfiI 16 GATTC G^A[AT]TC
826 GAATC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BsaI BsiYI BslI BsmAI
BssKI BstAPI BstNI BstYI CfoI
DdeI DpnII EcoNI EcoRII Fnu4HI
HaeII HaeIII HhaI HinP1I HinfI
ItaI MaeII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfcI SmlI Tru9I TseI
Tsp509I TspRI XhoII