(Circular) (Six-base) MAPSORT of: pFN21AB7408.seq Check: 4105 from: 1 to: 5887 pFN21AB7408 5887 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3230 1937 Size: 1293 4594 Acc65I G'GTAC_C Cuts at: 2400 3208 2400 Size: 808 5079 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3950 5334 5602 1632 Size: 2318 1384 268 1917 Fragments arranged by size: 2318 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 4786 5763 1203 Size: 3583 977 1327 Fragments arranged by size: 3583 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 2409 2498 1166 Size: 1243 89 4555 Fragments arranged by size: 4555 1243 89 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 3630 5202 3630 Size: 1572 4315 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 2557 2900 2923 3196 3422 3574 5683 1493 Size: 1064 343 23 273 226 152 2109 1697 Fragments arranged by size: 2109 1697 1064 343 273 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 2792 4441 160 Size: 872 1760 1649 1606 Fragments arranged by size: 1760 1649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 3212 1870 Size: 73 1269 4545 Fragments arranged by size: 4545 1269 73 BamHI G'GATC_C Cuts at: 3217 3584 3217 Size: 367 5520 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2196 2400 3208 4663 618 Size: 325 217 627 409 204 808 1455 1842 Fragments arranged by size: 1842 1455 808 627 409 325 217 204 BanII G_rGCy'C Cuts at: 1391 2397 3206 1391 Size: 1006 809 4072 Fragments arranged by size: 4072 1006 809 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2174 2285 928 Size: 971 275 111 4530 Fragments arranged by size: 4530 971 275 111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2141 2175 2252 2286 4078 4112 1903 Size: 34 204 34 77 34 1792 34 3678 Fragments arranged by size: 3678 1792 204 77 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2419 3037 5882 2419 Size: 618 2845 2424 Fragments arranged by size: 2845 2424 618 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 2138 3948 5104 1790 Size: 348 1810 1156 2573 Fragments arranged by size: 2573 1810 1156 348 BmgBI CAC'GTC Cuts at: 1318 2407 2500 1318 Size: 1089 93 4705 Fragments arranged by size: 4705 1089 93 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3618 4576 442 Size: 416 53 83 399 24 2201 958 1753 Fragments arranged by size: 2201 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2547 2646 2547 Size: 99 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2163 2274 4547 1239 Size: 924 111 2273 2579 Fragments arranged by size: 2579 2273 924 111 Bpu10I CC'TnA_GC Cuts at: 2486 2486 Size: 5887 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3018 3029 3154 4012 4877 5175 3018 Size: 11 125 858 865 298 3730 Fragments arranged by size: 3730 865 858 298 125 11 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2066 2139 2250 4550 882 Size: 617 567 73 111 2300 2219 Fragments arranged by size: 2300 2219 617 567 111 73 BsaAI yAC'GTr Cuts at: 493 1204 3678 493 Size: 711 2474 2702 Fragments arranged by size: 2702 2474 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3694 4076 4711 275 Size: 53 83 186 936 2161 382 635 1451 Fragments arranged by size: 2161 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4320 4718 4992 5139 5550 4320 Size: 398 274 147 411 4657 Fragments arranged by size: 4657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2384 2414 1178 Size: 30 165 30 981 30 4651 Fragments arranged by size: 4651 981 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5887 BseYI C'CCAG_C Cuts at: 5090 5586 5090 Size: 496 5391 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2747 1163 Size: 573 1011 4303 Fragments arranged by size: 4303 1011 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3948 4033 5104 3948 Size: 85 1071 4731 Fragments arranged by size: 4731 1071 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2397 3206 3948 4033 5104 1391 Size: 399 348 259 809 742 85 1071 2174 Fragments arranged by size: 2174 1071 809 742 399 348 259 85 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3242 844 Size: 2398 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2118 2340 3774 1394 Size: 429 295 222 1434 3507 Fragments arranged by size: 3507 1434 429 295 222 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 4531 4718 1700 Size: 110 219 2502 187 2869 Fragments arranged by size: 2869 2502 219 187 110 BsrGI T'GTAC_A Cuts at: 96 2872 96 Size: 2776 3111 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 3947 4959 3947 Size: 1012 4875 BstAPI GCAn_nnn'nTGC Cuts at: 794 2085 2196 2307 794 Size: 1291 111 111 4374 Fragments arranged by size: 4374 1291 111 111 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstXI CCAn_nnnn'nTGG Cuts at: 2411 2411 Size: 5887 BstYI r'GATC_y Cuts at: 1972 2419 2470 3037 3217 3584 3969 3986 5438 Size: 447 51 567 180 367 385 17 1452 Cuts at: 5438 5524 5536 5882 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 567 447 385 367 346 180 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2410 513 Size: 552 939 406 3990 Fragments arranged by size: 3990 939 552 406 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 2203 2314 2804 3403 4197 4217 2203 Size: 111 490 599 794 20 3873 Fragments arranged by size: 3873 794 599 490 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 2928 3191 2928 Size: 263 5624 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2796 3054 3334 Size: 54 1425 50 275 18 966 258 280 Cuts at: 3334 4223 5852 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 966 889 280 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2044 3145 3817 1353 Size: 691 1101 672 3423 Fragments arranged by size: 3423 1101 691 672 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4474 4848 4994 387 Size: 1064 138 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2274 3950 4547 5334 5602 1239 Size: 393 531 111 1676 597 787 268 1524 Fragments arranged by size: 1676 1524 787 597 531 393 268 111 EcoNI CCTnn'n_nnAGG Cuts at: 2107 2218 2329 2107 Size: 111 111 5665 Fragments arranged by size: 5665 111 111 EcoRI G'AATT_C Cuts at: 2557 3196 3574 2557 Size: 639 378 4870 Fragments arranged by size: 4870 639 378 EcoRV GAT'ATC Cuts at: 2827 2827 Size: 5887 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 2250 2361 2385 5034 1678 Size: 572 111 24 2649 2531 Fragments arranged by size: 2649 2531 572 111 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2816 2848 4528 4560 4602 Size: 32 135 32 1244 32 1680 32 42 Cuts at: 4602 4634 1373 Size: 32 2626 Fragments arranged by size: 2626 1680 1244 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2597 3231 3477 669 Size: 988 281 659 634 246 3079 Fragments arranged by size: 3079 988 659 634 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 2404 3212 2404 Size: 808 5079 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2798 10 Size: 54 1425 343 966 3099 Fragments arranged by size: 3099 1425 966 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2409 2480 2498 3084 3845 4204 4363 Size: 648 1243 71 18 586 761 359 159 Cuts at: 4363 518 Size: 2042 Fragments arranged by size: 2042 1243 761 648 586 359 159 71 18 MspA1I CmG'CkG Cuts at: 1606 3303 3980 5128 5373 1606 Size: 1697 677 1148 245 2120 Fragments arranged by size: 2120 1697 1148 677 245 NaeI GCC'GGC Cuts at: 2031 2031 Size: 5887 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4396 Fragments arranged by size: 4396 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NgoMIV G'CCGG_C Cuts at: 2029 2029 Size: 5887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 3245 4790 1132 Size: 97 2016 1545 2229 Fragments arranged by size: 2229 2016 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5887 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PstI C_TGCA'G Cuts at: 830 2222 2333 3239 830 Size: 1392 111 906 3478 Fragments arranged by size: 3478 1392 906 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SfcI C'TryA_G Cuts at: 826 1045 2107 2218 2329 3235 4370 5051 5242 Size: 219 1062 111 111 906 1135 681 191 Cuts at: 5242 826 Size: 1471 Fragments arranged by size: 1471 1135 1062 906 681 219 191 111 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3008 3033 3169 3991 4892 5154 Size: 197 926 1065 25 136 822 901 262 Cuts at: 5154 820 Size: 1553 Fragments arranged by size: 1553 1065 926 901 822 262 197 136 25 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 2734 3019 3811 5 Size: 47 2682 285 792 2081 Fragments arranged by size: 2682 2081 792 285 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5887 StyI C'CwwG_G Cuts at: 513 1065 2004 3326 513 Size: 552 939 1322 3074 Fragments arranged by size: 3074 1322 939 552 SwaI ATTT'AAAT Cuts at: 2928 2928 Size: 5887 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3914 3931 4084 4269 4714 325 Size: 1255 2334 17 153 185 445 1498 Fragments arranged by size: 2334 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2872 4133 5845 Size: 274 80 33 51 1443 895 1261 1712 Cuts at: 5845 96 Size: 138 Fragments arranged by size: 1712 1443 1261 895 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 2592 1316 Size: 1276 4611 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 4016 4016 Size: 5887 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI