Restriction map for
pFN21AB7406
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MslI MunI
NarI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 573 GTCTAC GT^[AC][GT]AC
AcyI 16 GGCGTC G[GA]^CG[CT]C
AflIII 83 ACGTGT A^C[GA][CT]GT
AlwI 25 GATCC GGATC(4/5)
AvaI 150 CTCGGG C^[CT]CG[GA]G
AvaII 3 GGACC G^G[AT]CC
BalI 206 TGGCCA TGG^CCA
BanI 55 GGCACC G^G[CT][GA]CC
BcnI 13 CCCGG CC^[GC]GG
BfaI 22 CTAG C^TAG
BmyI 54 GGGCAC G[AGT]GC[ACT]^C
BsaHI 16 GGCGTC G[GA]^CG[CT]C
BsoBI 150 CTCGGG C^[CT]CG[GA]G
Bsp1286I 54 GGGCAC G[AGT]GC[ACT]^C
BsrFI 131 GCCGGC [GA]^CCGG[CT]
BsrI 180 CCAGT ACTGG(1/-1)
BstF5I 437 CATCC GGATG(2/0)
BstNI 51 CCTGG CC^[AT]GG
BstXI 599 CCAGAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 24 AGATCC [GA]^GATC[CT]
Cfr10I 131 GCCGGC [GA]^CCGG[CT]
DraII 513 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 562 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 246 CCGTGG C^C[GA][CT]GG
EarI 505 GAAGAG CTCTTC(1/4)
EcoO109I 513 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 51 CCTGG ^CC[AT]GG
EcoRV 313 GATATC GAT^ATC
FokI 437 CATCC GGATG(9/13)
HgaI 17 GCGTC GACGC(5/10)
Hin1I 16 GGCGTC G[GA]^CG[CT]C
Ksp632I 505 GAAGAG CTCTTC(1/4)
MaeI 22 CTAG C^TAG
MaeII 83 ACGT A^CGT
MflI 24 AGATCC [GA]^GATC[CT]
MluNI 206 TGGCCA TGG^CCA
MscI 206 TGGCCA TGG^CCA
MseI 320 TTAA T^TAA
MvaI 51 CCTGG CC^[AT]GG
NaeI 131 GCCGGC GCC^GGC
NciI 13 CCCGG CC^[GC]GG
NgoMIV 131 GCCGGC G^CCGGC
PshAI 449 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 408 CTATAG C^T[GA][CT]AG
SmlI 136 CTCAAG C^T[CT][GA]AG
Tru9I 320 TTAA T^TAA
Tsp45I 638 GTCAC ^GT[GC]AC
Tsp509I 585 AATT ^AATT
XhoII 24 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbvI 108 GCTGC GCAGC(8/12)
144 GCTGC
BsrBI 193 GAGCGG CCGCTC(-3/-3)
226 GAGCGG
BssKI 13 CCCGG ^CC[ACGT]GG
51 CCTGG
DdeI 349 CTCAG C^T[ACGT]AG
634 CTGAG
DpnII 25 GATC ^GATC
355 GATC
EaeI 119 TGGCCG [CT]^GGCC[GA]
206 TGGCCA
EcoT14I 215 CCAAGG C^C[AT][AT]GG
517 CCTTGG
HapII 14 CCGG C^CGG
132 CCGG
HpaII 14 CCGG C^CGG
132 CCGG
HphI 538 GGTGA GGTGA(8/7)
467 TCACC
MaeIII 413 GTAAC ^GT[ACGT]AC
638 GTCAC
MboI 25 GATC ^GATC
355 GATC
MspI 14 CCGG C^CGG
132 CCGG
NdeII 25 GATC ^GATC
355 GATC
PleI 376 GACTC GAGTC(4/5)
636 GAGTC
Sau3AI 25 GATC ^GATC
355 GATC
ScrFI 13 CCCGG CC^[ACGT]GG
51 CCTGG
StyI 215 CCAAGG C^C[AT][AT]GG
517 CCTTGG
TfiI 427 GAATC G^A[AT]TC
614 GATTC
TseI 108 GCTGC G^C[AT]GC
144 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AccII AciI AluI BpmI BsaJI
BsiYI BslI BstUI Cac8I CfoI
Cfr13I FauI Fnu4HI HaeIII HhaI
HinP1I HinfI ItaI MboII MnlI
MspA1I MvnI MwoI NlaIV PvuII
Sau96I TspRI