Restriction map for
pFN21AB7402
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AviII AvrII BamHI BbeI
BbsI BbvCI BbvI BcnI BglI
BglII BlnI BpuAI BsaHI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI Fnu4HI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI ItaI
KasI Ksp632I KspI MfeI MluI
MroI MspA1I MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TseI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 465 GGTACC G^GTACC
AccII 601 CGCG CG^CG
AcsI 840 GAATTT [GA]^AATT[CT]
AflII 214 CTTAAG C^TTAAG
AlwNI 514 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 723 GGGCCC GGGCC^C
ApoI 840 GAATTT [GA]^AATT[CT]
Asp700I 195 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 465 GGTACC G^GTACC
AspHI 350 GAGCAC G[AT]GC[AT]^C
AspI 152 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 22 CCCGAG C^[CT]CG[GA]G
BbrPI 117 CACGTG CAC^GTG
BcgI 632 CGACCCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 778 GTATCC GTATCC(6/5)
BclI 233 TGATCA T^GATCA
BfaI 992 CTAG C^TAG
BfrI 214 CTTAAG C^TTAAG
BlpI 642 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 642 GCTTAGC GC^T[ACGT]AGC
BsaAI 117 CACGTG [CT]AC^GT[GA]
BsaBI 678 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 157 GGTCTC GGTCTC(1/5)
BsaWI 527 ACCGGA [AT]^CCGG[AT]
BsiHKAI 350 GAGCAC G[AT]GC[AT]^C
BsoBI 22 CCCGAG C^[CT]CG[GA]G
Bsp1407I 269 TGTACA T^GTACA
BspHI 734 TCATGA T^CATGA
BspMI 246 GCAGGT ACCTGC(4/8)
BsrDI 1060 GCAATG GCAATG(2/0)
BsrGI 269 TGTACA T^GTACA
BstUI 601 CGCG CG^CG
CelII 642 GCTTAGC GC^T[ACGT]AGC
DsaI 867 CCGTGG C^C[GA][CT]GG
FbaI 233 TGATCA T^GATCA
HapII 528 CCGG C^CGG
HinfI 106 GATTC G^A[ACGT]TC
HpaII 528 CCGG C^CGG
KpnI 465 GGTACC GGTAC^C
MaeI 992 CTAG C^TAG
MaeIII 662 GTGAC ^GT[ACGT]AC
MamI 678 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 837 GAAGA GAAGA(8/7)
MspI 528 CCGG C^CGG
MvnI 601 CGCG CG^CG
PflFI 152 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 117 CACGTG CAC^GTG
PmlI 117 CACGTG CAC^GTG
RcaI 734 TCATGA T^CATGA
SfcI 393 CTATAG C^T[GA][CT]AG
SspBI 269 TGTACA T^GTACA
TfiI 106 GATTC G^A[AT]TC
Tsp45I 662 GTGAC ^GT[GC]AC
Tth111I 152 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 195 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 270 GTAC GT^AC
466 GTAC
BalI 668 TGGCCA TGG^CCA
848 TGGCCA
BanII 723 GGGCCC G[GA]GC[CT]^C
863 GAGCCC
BseRI 225 CTCCTC GAGGAG(10/8)
769 GAGGAG
BsgI 330 GTGCAG GTGCAG(16/14)
828 GTGCAG
BstYI 254 AGATCC [GA]^GATC[CT]
377 AGATCC
DraII 722 AGGGCCC [GA]G^G[ACGT]CC[CT]
723 GGGCCCT
DraIII 114 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1076 CACTTAGTG
EaeI 668 TGGCCA [CT]^GGCC[GA]
848 TGGCCA
EcoNI 714 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
807 CCTGAATGAGG
EcoO109I 722 AGGGCCC [GA]G^G[ACGT]CC[CT]
723 GGGCCCT
HgaI 337 GCGTC GACGC(5/10)
602 GCGTC
HphI 1044 GGTGA GGTGA(8/7)
113 TCACC
MaeII 118 ACGT A^CGT
388 ACGT
MflI 254 AGATCC [GA]^GATC[CT]
377 AGATCC
MluNI 668 TGGCCA TGG^CCA
848 TGGCCA
MscI 668 TGGCCA TGG^CCA
848 TGGCCA
MseI 215 TTAA T^TAA
1013 TTAA
MslI 87 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
510 CACTCAGATG
RsaI 270 GTAC GT^AC
466 GTAC
SmlI 142 CTCAAG C^T[CT][GA]AG
214 CTTAAG
TaqI 686 TCGA T^CGA
857 TCGA
Tru9I 215 TTAA T^TAA
1013 TTAA
Tsp509I 274 AATT ^AATT
841 AATT
TthHB8I 686 TCGA T^CGA
857 TCGA
XhoII 254 AGATCC [GA]^GATC[CT]
377 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I FokI HaeIII MboI
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TspRI XcmI