(Circular) (Six-base) MAPSORT of: pFN21AB7402.seq Check: 8680 from: 1 to: 5794 pFN21AB7402 5794 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3604 278 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3137 1937 Size: 1200 4594 Acc65I G'GTAC_C Cuts at: 2470 3115 2470 Size: 645 5149 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3857 5241 5509 1632 Size: 2225 1384 268 1917 Fragments arranged by size: 2225 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2219 820 Size: 197 1202 4395 Fragments arranged by size: 4395 1202 197 AflIII A'CryG_T Cuts at: 1203 4693 5670 1203 Size: 3490 977 1327 Fragments arranged by size: 3490 1327 977 AgeI A'CCGG_T Cuts at: 4625 4625 Size: 5794 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5794 AlwNI CAG_nnn'CTG Cuts at: 2524 3537 5109 2524 Size: 1013 1572 3209 Fragments arranged by size: 3209 1572 1013 ApaI G_GGCC'C Cuts at: 2732 2732 Size: 5794 ApaLI G'TGCA_C Cuts at: 3851 5007 3851 Size: 1156 4638 ApoI r'AATT_y Cuts at: 1493 2845 3103 3329 3481 5590 1493 Size: 1352 258 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 258 226 152 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 2027 3119 1870 Size: 73 84 1092 4545 Fragments arranged by size: 4545 1092 84 73 BamHI G'GATC_C Cuts at: 3124 3491 3124 Size: 367 5427 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 2443 2470 2546 3115 Size: 325 217 627 317 339 27 76 569 Cuts at: 3115 4570 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 569 339 325 317 217 76 27 BanII G_rGCy'C Cuts at: 1391 2732 2872 3113 1391 Size: 1341 140 241 4072 Fragments arranged by size: 4072 1341 241 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4823 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2626 2660 3985 4019 1903 Size: 34 50 34 605 34 1325 34 3678 Fragments arranged by size: 3678 1325 605 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2794 3686 4896 2794 Size: 892 1210 3692 Fragments arranged by size: 3692 1210 892 BclI T'GATC_A Cuts at: 1548 2238 1548 Size: 690 5104 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4405 136 Size: 107 122 71 631 605 2733 1525 Fragments arranged by size: 2733 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5789 5789 Size: 5794 BlpI GC'TnA_GC Cuts at: 2648 3211 2648 Size: 563 5231 Bme1580I G_kGCm'C Cuts at: 1790 2715 2732 3855 5011 1790 Size: 925 17 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 925 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3525 4483 442 Size: 416 53 83 399 24 2108 958 1753 Fragments arranged by size: 2108 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2741 2903 4454 1239 Size: 823 679 162 1551 2579 Fragments arranged by size: 2579 1551 823 679 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2132 3919 4784 5082 2132 Size: 1787 865 298 2844 Fragments arranged by size: 2844 1787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2168 4457 882 Size: 617 669 2289 2219 Fragments arranged by size: 2289 2219 669 617 BsaAI yAC'GTr Cuts at: 493 1204 2124 3585 493 Size: 711 920 1461 2702 Fragments arranged by size: 2702 1461 920 711 BsaBI GATnn'nnATC Cuts at: 2687 5788 2687 Size: 3101 2693 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3601 3983 4618 275 Size: 53 83 186 936 2068 382 635 1451 Fragments arranged by size: 2068 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2532 4227 4625 4899 5046 5457 2532 Size: 1695 398 274 147 411 2869 Fragments arranged by size: 2869 1695 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 2543 2573 2753 Size: 30 165 30 915 30 195 30 180 Cuts at: 2753 2783 1178 Size: 30 4189 Fragments arranged by size: 4189 915 195 180 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 2789 1186 Size: 1035 568 4191 Fragments arranged by size: 4191 1035 568 BseYI C'CCAG_C Cuts at: 4997 5493 4997 Size: 496 5298 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2356 2854 1163 Size: 573 620 498 4103 Fragments arranged by size: 4103 620 573 498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3244 4005 5033 1540 Size: 275 186 1243 761 1028 2301 Fragments arranged by size: 2301 1243 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2359 3855 3940 5011 2359 Size: 1496 85 1071 3142 Fragments arranged by size: 3142 1496 1071 85 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 2715 2732 2872 3113 3855 3940 Size: 399 569 356 17 140 241 742 85 Cuts at: 3940 5011 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 569 399 356 241 140 85 17 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 2739 3683 5413 2739 Size: 944 1730 3120 Fragments arranged by size: 3120 1730 944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2242 3149 844 Size: 1398 907 3489 Fragments arranged by size: 3489 1398 907 BsrBI CCG'CTC Cuts at: 1394 1823 3681 1394 Size: 429 1858 3507 Fragments arranged by size: 3507 1858 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3072 4289 4463 65 Size: 1183 1824 1217 174 1396 Fragments arranged by size: 1824 1396 1217 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4438 4625 1700 Size: 110 2628 187 2869 Fragments arranged by size: 2869 2628 187 110 BsrGI T'GTAC_A Cuts at: 96 2274 96 Size: 2178 3616 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5794 BssSI C'ACGA_G Cuts at: 3854 4866 3854 Size: 1012 4782 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5794 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5794 BstYI r'GATC_y Cuts at: 1972 2259 2382 3124 3491 3876 3893 5345 5431 Size: 287 123 742 367 385 17 1452 86 Cuts at: 5431 5443 5789 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 742 385 367 346 287 123 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2872 513 Size: 552 1807 3435 Fragments arranged by size: 3435 1807 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5055 Fragments arranged by size: 5055 683 56 BtsI GCAGTG_nn' Cuts at: 2344 3310 4104 4124 2344 Size: 966 794 20 4014 Fragments arranged by size: 4014 966 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5759 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DraIII CAC_nnn'GTG Cuts at: 2124 3086 2124 Size: 962 4832 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4801 809 Size: 507 3485 1802 Fragments arranged by size: 3485 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2673 2853 3241 Size: 54 1425 50 275 18 843 180 388 Cuts at: 3241 4130 5759 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 843 388 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3241 1537 Size: 275 1429 4090 Fragments arranged by size: 4090 1429 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3724 1353 Size: 2371 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2501 4381 4755 4901 387 Size: 1064 138 912 1880 374 146 1280 Fragments arranged by size: 1880 1280 1064 912 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2741 2903 3857 4454 5241 5509 Size: 393 430 679 162 954 597 787 268 Cuts at: 5509 1239 Size: 1524 Fragments arranged by size: 1524 954 787 679 597 430 393 268 162 EcoNI CCTnn'n_nnAGG Cuts at: 2723 2816 2723 Size: 93 5701 EcoO109I rG'GnC_Cy Cuts at: 2728 2729 2728 Size: 1 5793 EcoRI G'AATT_C Cuts at: 3103 3481 3103 Size: 378 5416 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 4941 1678 Size: 3263 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2768 2800 3043 Size: 32 135 32 576 32 588 32 243 Cuts at: 3043 3075 4435 4467 4509 4541 1373 Size: 32 1360 32 42 32 2626 Fragments arranged by size: 2626 1360 588 576 243 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3138 3384 669 Size: 988 281 1200 246 3079 Fragments arranged by size: 3079 1200 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KpnI G_GTAC'C Cuts at: 2474 3119 2474 Size: 645 5149 MfeI C'AATT_G Cuts at: 3393 3393 Size: 5794 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4908 5092 1305 Size: 3603 184 2007 Fragments arranged by size: 3603 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 2855 10 Size: 54 1425 343 843 180 2949 Fragments arranged by size: 2949 1425 843 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2096 2519 3752 4111 4270 518 Size: 648 843 87 423 1233 359 159 2042 Fragments arranged by size: 2042 1233 843 648 423 359 159 87 MspA1I CmG'CkG Cuts at: 1606 3210 3887 5035 5280 1606 Size: 1604 677 1148 245 2120 Fragments arranged by size: 2120 1604 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5242 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5794 NotI GC'GGCC_GC Cuts at: 3241 3241 Size: 5794 NspI r_CATG'y Cuts at: 1132 1229 3152 4697 1132 Size: 97 1923 1545 2229 Fragments arranged by size: 2229 1923 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5794 PciI A'CATG_T Cuts at: 4693 4693 Size: 5794 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5794 PfoI T'CCnGG_A Cuts at: 2409 2718 2880 2409 Size: 309 162 5323 Fragments arranged by size: 5323 309 162 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PmlI CAC'GTG Cuts at: 2124 2124 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3863 3895 5376 5408 3863 Size: 32 1481 32 4249 Fragments arranged by size: 4249 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5794 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PspOMI G'GGCC_C Cuts at: 2728 2728 Size: 5794 PstI C_TGCA'G Cuts at: 830 3146 830 Size: 2316 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 4042 5754 4042 Size: 1712 4082 SfcI C'TryA_G Cuts at: 826 1045 2398 3142 4277 4958 5149 826 Size: 219 1353 744 1135 681 191 1471 Fragments arranged by size: 1471 1353 1135 744 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5794 SmaI CCC'GGG Cuts at: 1872 3121 1872 Size: 1249 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 2219 3898 4799 5061 820 Size: 197 926 204 72 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 204 197 72 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 3152 1132 Size: 2020 3774 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5794 StyI C'CwwG_G Cuts at: 513 1065 2298 2419 2562 2764 3233 513 Size: 552 1233 121 143 202 469 3074 Fragments arranged by size: 3074 1233 552 469 202 143 121 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2991 3821 3838 3991 4176 4621 325 Size: 1255 1411 830 17 153 185 445 1498 Fragments arranged by size: 1498 1411 1255 830 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 4040 5752 Size: 274 80 33 51 1443 297 1766 1712 Cuts at: 5752 96 Size: 138 Fragments arranged by size: 1766 1712 1443 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 5754 5786 2318 Size: 32 3404 32 2326 Fragments arranged by size: 3404 2326 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2160 1316 Size: 844 4950 XbaI T'CTAG_A Cuts at: 3130 3130 Size: 5794 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2305 2580 2974 2305 Size: 275 394 5125 Fragments arranged by size: 5125 394 275 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5794 XmaI C'CCGG_G Cuts at: 1870 3119 1870 Size: 1249 4545 XmnI GAAnn'nnTTC Cuts at: 2204 3923 2204 Size: 1719 4075 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3602 276 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI