Restriction map for
pFN21AB7402
(Linear) (Six-base) MAPSORT of: KIBB7402.seq Check: 8368 from: 1 to: 1089
KIBB7402.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
Acc65I G'GTAC_C
Cuts at: 0 465 1089
Size: 465 624
AflII C'TTAA_G
Cuts at: 0 214 1089
Size: 214 875
AlwNI CAG_nnn'CTG
Cuts at: 0 519 1089
Size: 519 570
ApaI G_GGCC'C
Cuts at: 0 727 1089
Size: 727 362
ApoI r'AATT_y
Cuts at: 0 840 1089
Size: 840 249
AvaI C'yCGr_G
Cuts at: 0 22 1089
Size: 22 1067
BanI G'GyrC_C
Cuts at: 0 99 438 465 541 1089
Size: 99 339 27 76 548
Fragments arranged by size:
548 339 99 76 27
BanII G_rGCy'C
Cuts at: 0 727 867 1089
Size: 727 140 222
Fragments arranged by size:
727 222 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 621 655 1089
Size: 621 34 434
Fragments arranged by size:
621 434 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 789 1089
Size: 789 300
BclI T'GATC_A
Cuts at: 0 233 1089
Size: 233 856
BlpI GC'TnA_GC
Cuts at: 0 643 1089
Size: 643 446
Bme1580I G_kGCm'C
Cuts at: 0 710 727 1089
Size: 710 17 362
Fragments arranged by size:
710 362 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 736 898 1089
Size: 57 679 162 191
Fragments arranged by size:
679 191 162 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1089
Size: 127 962
BsaI GGTCTCn'nnnn_
Cuts at: 0 163 1089
Size: 163 926
BsaAI yAC'GTr
Cuts at: 0 119 1089
Size: 119 970
BsaBI GATnn'nnATC
Cuts at: 0 682 1089
Size: 682 407
BsaWI w'CCGG_w
Cuts at: 0 527 1089
Size: 527 562
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 538 568 748 778 1089
Size: 313 30 195 30 180 30 311
Fragments arranged by size:
313 311 195 180 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 784 1089
Size: 216 568 305
Fragments arranged by size:
568 305 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 849 1089
Size: 351 498 240
Fragments arranged by size:
498 351 240
BsiHKAI G_wGCw'C
Cuts at: 0 354 1089
Size: 354 735
Bsp1286I G_dGCh'C
Cuts at: 0 354 710 727 867 1089
Size: 354 356 17 140 222
Fragments arranged by size:
356 354 222 140 17
BspHI T'CATG_A
Cuts at: 0 734 1089
Size: 734 355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 237 1089
Size: 237 852
BsrDI GCAATG_nn'
Cuts at: 0 1067 1089
Size: 1067 22
BsrGI T'GTAC_A
Cuts at: 0 269 1089
Size: 269 820
BstYI r'GATC_y
Cuts at: 0 254 377 1089
Size: 254 123 712
Fragments arranged by size:
712 254 123
BtgI C'CryG_G
Cuts at: 0 867 1089
Size: 867 222
BtsI GCAGTG_nn'
Cuts at: 0 339 1089
Size: 339 750
DraIII CAC_nnn'GTG
Cuts at: 0 119 1081 1089
Size: 119 962 8
Fragments arranged by size:
962 119 8
EaeI y'GGCC_r
Cuts at: 0 668 848 1089
Size: 668 180 241
Fragments arranged by size:
668 241 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 496 1089
Size: 496 593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 736 898 1089
Size: 57 679 162 191
Fragments arranged by size:
679 191 162 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 718 811 1089
Size: 718 93 278
Fragments arranged by size:
718 278 93
EcoO109I rG'GnC_Cy
Cuts at: 0 723 724 1089
Size: 723 1 365
Fragments arranged by size:
723 365 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 763 795 1038 1070 1089
Size: 143 32 588 32 243 32 19
Fragments arranged by size:
588 243 143 32 32 32 19
KpnI G_GTAC'C
Cuts at: 0 469 1089
Size: 469 620
MscI TGG'CCA
Cuts at: 0 670 850 1089
Size: 670 180 239
Fragments arranged by size:
670 239 180
MslI CAynn'nnrTG
Cuts at: 0 91 514 1089
Size: 91 423 575
Fragments arranged by size:
575 423 91
PfoI T'CCnGG_A
Cuts at: 0 404 713 875 1089
Size: 404 309 162 214
Fragments arranged by size:
404 309 214 162
PmlI CAC'GTG
Cuts at: 0 119 1089
Size: 119 970
PspOMI G'GGCC_C
Cuts at: 0 723 1089
Size: 723 366
SfcI C'TryA_G
Cuts at: 0 393 1089
Size: 393 696
SmlI C'TyrA_G
Cuts at: 0 142 214 1089
Size: 142 72 875
Fragments arranged by size:
875 142 72
StyI C'CwwG_G
Cuts at: 0 293 414 557 759 1089
Size: 293 121 143 202 330
Fragments arranged by size:
330 293 202 143 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 986 1089
Size: 986 103
TatI w'GTAC_w
Cuts at: 0 269 1089
Size: 269 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 1089
Size: 313 32 744
Fragments arranged by size:
744 313 32
Tth111I GACn'n_nGTC
Cuts at: 0 155 1089
Size: 155 934
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 300 575 969 1089
Size: 300 275 394 120
Fragments arranged by size:
394 300 275 120
XmnI GAAnn'nnTTC
Cuts at: 0 199 1089
Size: 199 890
Enzymes that do cut:
Acc65I AflII AlwNI ApaI ApoI AvaI BanI BanII
BcgI BciVI BclI BlpI Bme1580I BpmI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BsrGI BstYI BtgI BtsI DraIII
EaeI EciI Eco57MI EcoNI EcoO109I Hin4I KpnI MscI
MslI PfoI PmlI PspOMI SfcI SmlI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BfrBI BglI
BglII BmgBI BmrI BmtI BplI Bpu10I BsaHI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XhoI XmaI ZraI