################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI MaeI MaeII MaeIII MamI MflI MluI MluNI MroI MscI MseI NarI NciI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 433 GTAC GT^AC Aor51HI 173 AGCGCT AGC^GCT ApaI 195 GGGCCC GGGCC^C AspHI 451 GAGCTC G[AT]GC[AT]^C AvaII 75 GGTCC G^G[AT]CC BanI 427 GGCACC G^G[CT][GA]CC BcgI 501 CGATCAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 141 GCTGAGC GC^T[ACGT]AGC BpmI 454 CTCCAG CTGGAG(16/14) Bpu1102I 141 GCTGAGC GC^T[ACGT]AGC BsiHKAI 451 GAGCTC G[AT]GC[AT]^C BsmAI 240 GTCTC GTCTC(1/5) BsrFI 201 GCCGGC [GA]^CCGG[CT] BssSI 298 CACGAG CACGAG(-5/-1) CelII 141 GCTGAGC GC^T[ACGT]AGC Cfr10I 201 GCCGGC [GA]^CCGG[CT] DpnII 502 GATC ^GATC DraII 194 GGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 396 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 105 CCGCGG C^C[GA][CT]GG Eco47III 173 AGCGCT AGC^GCT EcoNI 285 CCTATGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 194 GGGGCCC [GA]G^G[ACGT]CC[CT] HapII 202 CCGG C^CGG HpaII 202 CCGG C^CGG KspI 105 CCGCGG CCGC^GG MboI 502 GATC ^GATC MfeI 512 CAATTG C^AATTG MspI 202 CCGG C^CGG MunI 512 CAATTG C^AATTG NaeI 201 GCCGGC GCC^GGC NdeII 502 GATC ^GATC NgoMIV 201 GCCGGC G^CCGGC NotI 107 GCGGCCGC GC^GGCCGC NspI 134 GCATGT [GA]CATG^[CT] PvuII 441 CAGCTG CAG^CTG RsaI 433 GTAC GT^AC SacI 451 GAGCTC GAGCT^C SacII 105 CCGCGG CCGC^GG Sau3AI 502 GATC ^GATC TaqI 464 TCGA T^CGA TspRI 28 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 464 TCGA T^CGA XcmI 377 CCATCGTGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BbsI 352 GTCTTC GAAGAC(2/6) 361 GTCTTC BbvI 113 GCTGC GCAGC(8/12) 246 GCTGC BpuAI 352 GTCTTC GAAGAC(2/6) 361 GTCTTC BsgI 160 GTGCAG GTGCAG(16/14) 184 GTGCAG BsiEI 108 CGGCCG CG[GA][CT]^CG 203 CGGCCG BsmFI 294 GGGAC GGGAC(10/14) 76 GTCCC BsrDI 366 CATTGC GCAATG(2/0) 553 GCAATG DdeI 26 CTCAG C^T[ACGT]AG 142 CTGAG EagI 108 CGGCCG C^GGCCG 203 CGGCCG EclXI 108 CGGCCG C^GGCCG 203 CGGCCG Eco52I 108 CGGCCG C^GGCCG 203 CGGCCG FauI 78 CCCGC CCCGC(4/6) 198 CCCGC MslI 375 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 439 CACAGCTGTG NlaIII 135 CATG CATG^ 399 CATG TseI 113 GCTGC G^C[AT]GC 246 GCTGC Tsp509I 13 AATT ^AATT 513 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AluI BanII BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I EaeI EarI EcoRII Fnu4HI HaeII HaeIII HhaI HinP1I HphI ItaI Ksp632I MboII MnlI MspA1I MvaI MvnI MwoI NlaIV SapI Sau96I ScrFI