(Circular) (Six-base) MAPSORT of: pFN21AB7401.seq Check: 2891 from: 1 to: 5296 pFN21AB7401 5296 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3106 278 Size: 53 83 186 936 1570 2468 Fragments arranged by size: 2468 1570 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2639 1937 Size: 702 4594 Acc65I G'GTAC_C Cuts at: 2617 2617 Size: 5296 AclI AA'CG_TT Cuts at: 1563 3423 3796 1563 Size: 1860 373 3063 Fragments arranged by size: 3063 1860 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2206 3359 4743 5011 1632 Size: 574 1153 1384 268 1917 Fragments arranged by size: 1917 1384 1153 574 268 AfeI AGC'GCT Cuts at: 2180 2180 Size: 5296 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5099 AflIII A'CryG_T Cuts at: 1203 4195 5172 1203 Size: 2992 977 1327 Fragments arranged by size: 2992 1327 977 AgeI A'CCGG_T Cuts at: 4127 4127 Size: 5296 AhdI GACnn_n'nnGTC Cuts at: 4025 4025 Size: 5296 AleI CACnn'nnGTG Cuts at: 1166 2384 2448 1166 Size: 1218 64 4014 Fragments arranged by size: 4014 1218 64 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5296 AlwNI CAG_nnn'CTG Cuts at: 3039 4611 3039 Size: 1572 3724 ApaI G_GGCC'C Cuts at: 2204 2204 Size: 5296 ApaLI G'TGCA_C Cuts at: 3353 4509 3353 Size: 1156 4140 ApoI r'AATT_y Cuts at: 1493 2605 2831 2983 5092 1493 Size: 1112 226 152 2109 1697 Fragments arranged by size: 2109 1697 1112 226 152 AseI AT'TA_AT Cuts at: 160 1032 3850 160 Size: 872 2818 1606 Fragments arranged by size: 2818 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5296 AvaI C'yCGr_G Cuts at: 1870 1943 2621 1870 Size: 73 678 4545 Fragments arranged by size: 4545 678 73 BamHI G'GATC_C Cuts at: 2626 2993 2626 Size: 367 4929 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2432 2617 4072 618 Size: 325 217 627 645 185 1455 1842 Fragments arranged by size: 1842 1455 645 627 325 217 185 BanII G_rGCy'C Cuts at: 1391 2204 2312 2460 2537 2615 1391 Size: 813 108 148 77 78 4072 Fragments arranged by size: 4072 813 148 108 78 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2350 2359 928 Size: 971 451 9 3865 Fragments arranged by size: 3865 971 451 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2495 2529 3487 3521 1903 Size: 34 558 34 958 34 3678 Fragments arranged by size: 3678 958 558 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3188 4398 3188 Size: 1210 4086 BclI T'GATC_A Cuts at: 1548 1548 Size: 5296 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3907 136 Size: 107 122 71 631 605 2235 1525 Fragments arranged by size: 2235 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5291 5291 Size: 5296 BlpI GC'TnA_GC Cuts at: 2147 2713 2147 Size: 566 4730 Bme1580I G_kGCm'C Cuts at: 1790 2204 2435 3357 4513 1790 Size: 414 231 922 1156 2573 Fragments arranged by size: 2573 1156 922 414 231 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5296 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3027 3985 442 Size: 416 53 83 399 24 1610 958 1753 Fragments arranged by size: 1753 1610 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5296 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2444 3956 1239 Size: 1205 1512 2579 Fragments arranged by size: 2579 1512 1205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3421 4286 4584 3421 Size: 865 298 4133 Fragments arranged by size: 4133 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3959 882 Size: 617 2460 2219 Fragments arranged by size: 2460 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3087 493 Size: 711 1883 2702 Fragments arranged by size: 2702 1883 711 BsaBI GATnn'nnATC Cuts at: 5290 5290 Size: 5296 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3103 3485 4120 275 Size: 53 83 186 936 1570 382 635 1451 Fragments arranged by size: 1570 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3729 4127 4401 4548 4959 3729 Size: 398 274 147 411 4066 Fragments arranged by size: 4066 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2294 2324 1178 Size: 30 165 30 891 30 4150 Fragments arranged by size: 4150 891 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5296 BseYI C'CCAG_C Cuts at: 2479 2563 4499 4995 2479 Size: 84 1936 496 2780 Fragments arranged by size: 2780 1936 496 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2210 1163 Size: 573 450 24 4249 Fragments arranged by size: 4249 573 450 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2116 2211 2746 3507 4535 1540 Size: 275 186 115 95 535 761 1028 2301 Fragments arranged by size: 2301 1028 761 535 275 186 115 95 BsiHKAI G_wGCw'C Cuts at: 2460 3357 3442 4513 2460 Size: 897 85 1071 3243 Fragments arranged by size: 3243 1071 897 85 BsmI GAATG_Cn' Cuts at: 2807 2900 2807 Size: 93 5203 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2312 2435 2460 2537 2615 3357 Size: 399 414 108 123 25 77 78 742 Cuts at: 3357 3442 4513 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 414 399 123 108 85 78 77 25 BspEI T'CCGG_A Cuts at: 4959 4959 Size: 5296 BspHI T'CATG_A Cuts at: 3185 4915 3185 Size: 1730 3566 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2651 844 Size: 1807 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3183 1394 Size: 429 1360 3507 Fragments arranged by size: 3507 1360 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2370 2565 3791 3965 65 Size: 1183 1122 195 1226 174 1396 Fragments arranged by size: 1396 1226 1183 1122 195 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2206 3940 4127 1700 Size: 110 396 1734 187 2869 Fragments arranged by size: 2869 1734 396 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5296 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5296 BssSI C'ACGA_G Cuts at: 2303 3356 4368 2303 Size: 1053 1012 3231 Fragments arranged by size: 3231 1053 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5296 BstBI TT'CG_AA Cuts at: 4100 4100 Size: 5296 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5296 BstYI r'GATC_y Cuts at: 1972 2626 2993 3378 3395 4847 4933 4945 5291 Size: 654 367 385 17 1452 86 12 346 Cuts at: 5291 1972 Size: 1977 Fragments arranged by size: 1977 1452 654 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2110 513 Size: 552 939 106 3699 Fragments arranged by size: 3699 939 552 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4557 Fragments arranged by size: 4557 683 56 BtsI GCAGTG_nn' Cuts at: 2812 3606 3626 2812 Size: 794 20 4482 Fragments arranged by size: 4482 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5296 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5261 DraI TTT'AAA Cuts at: 2600 2600 Size: 5296 DraIII CAC_nnn'GTG Cuts at: 2406 2406 Size: 5296 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4303 809 Size: 507 2987 1802 Fragments arranged by size: 2987 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2113 2208 Size: 54 1425 50 275 18 268 15 95 Cuts at: 2208 2256 2743 3632 5261 8 Size: 48 487 889 1629 43 Fragments arranged by size: 1629 1425 889 487 275 268 95 54 50 48 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2113 2208 2743 1537 Size: 275 301 95 535 4090 Fragments arranged by size: 4090 535 301 275 95 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 2288 2448 3226 1353 Size: 779 156 160 778 3423 Fragments arranged by size: 3423 779 778 160 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2311 2485 3883 4257 4403 387 Size: 1064 138 722 174 1398 374 146 1280 Fragments arranged by size: 1398 1280 1064 722 374 174 146 138 EcoICRI GAG'CTC Cuts at: 2458 2458 Size: 5296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2444 3359 3956 4743 5011 1239 Size: 393 574 238 915 597 787 268 1524 Fragments arranged by size: 1524 915 787 597 574 393 268 238 EcoNI CCTnn'n_nnAGG Cuts at: 2294 2294 Size: 5296 EcoO109I rG'GnC_Cy Cuts at: 2200 2200 Size: 5296 EcoRI G'AATT_C Cuts at: 2605 2983 2605 Size: 378 4918 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2570 2570 Size: 5296 FspI TGC'GCA Cuts at: 3802 3802 Size: 5296 HaeII r_GCGC'y Cuts at: 1678 2012 2154 2182 2349 4443 1678 Size: 334 142 28 167 2094 2531 Fragments arranged by size: 2531 2094 334 167 142 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3937 3969 4011 4043 1373 Size: 32 135 32 2365 32 42 32 2626 Fragments arranged by size: 2626 2365 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2640 2886 669 Size: 988 281 702 246 3079 Fragments arranged by size: 3079 988 702 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5296 HpaI GTT'AAC Cuts at: 2886 2886 Size: 5296 KpnI G_GTAC'C Cuts at: 2621 2621 Size: 5296 MfeI C'AATT_G Cuts at: 2517 2895 2517 Size: 378 4918 MluI A'CGCG_T Cuts at: 5172 5172 Size: 5296 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4410 4594 1305 Size: 3105 184 2007 Fragments arranged by size: 3105 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3474 Fragments arranged by size: 3474 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2448 3254 3613 3772 518 Size: 648 1218 64 806 359 159 2042 Fragments arranged by size: 2042 1218 806 648 359 159 64 MspA1I CmG'CkG Cuts at: 1606 2086 2112 2118 2448 2712 3389 4537 4782 Size: 480 26 6 330 264 677 1148 245 Cuts at: 4782 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 480 330 264 245 26 6 NaeI GCC'GGC Cuts at: 2208 2208 Size: 5296 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3805 Fragments arranged by size: 3805 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5296 NgoMIV G'CCGG_C Cuts at: 2206 2206 Size: 5296 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5296 NotI GC'GGCC_GC Cuts at: 2113 2743 2113 Size: 630 4666 NspI r_CATG'y Cuts at: 1132 1229 2143 2654 4199 1132 Size: 97 914 511 1545 2229 Fragments arranged by size: 2229 1545 914 511 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5296 PciI A'CATG_T Cuts at: 4195 4195 Size: 5296 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5296 PfoI T'CCnGG_A Cuts at: 2460 2460 Size: 5296 PmeI GTTT'AAAC Cuts at: 2600 2600 Size: 5296 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3365 3397 4878 4910 3365 Size: 32 1481 32 3751 Fragments arranged by size: 3751 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5296 PsiI TTA'TAA Cuts at: 2866 2866 Size: 5296 PspOMI G'GGCC_C Cuts at: 2200 2200 Size: 5296 PstI C_TGCA'G Cuts at: 830 2648 830 Size: 1818 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5296 PvuII CAG'CTG Cuts at: 2448 2448 Size: 5296 SacI G_AGCT'C Cuts at: 2460 2460 Size: 5296 SacII CC_GC'GG Cuts at: 2113 2113 Size: 5296 SalI G'TCGA_C Cuts at: 1936 2638 1936 Size: 702 4594 SapI GCTCTTCn'nnn_ Cuts at: 2132 2288 2448 2132 Size: 156 160 4980 Fragments arranged by size: 4980 160 156 SbfI CC_TGCA'GG Cuts at: 2648 2648 Size: 5296 ScaI AGT'ACT Cuts at: 3544 5256 3544 Size: 1712 3584 SfcI C'TryA_G Cuts at: 826 1045 2644 3779 4460 4651 826 Size: 219 1599 1135 681 191 1471 Fragments arranged by size: 1599 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5296 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5296 SmaI CCC'GGG Cuts at: 1872 2623 1872 Size: 751 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3400 4301 4563 820 Size: 197 926 1457 901 262 1553 Fragments arranged by size: 1553 1457 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3087 493 Size: 2594 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5296 SphI G_CATG'C Cuts at: 1132 2654 1132 Size: 1522 3774 SspI AAT'ATT Cuts at: 5 52 3220 5 Size: 47 3168 2081 Fragments arranged by size: 3168 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5296 StyI C'CwwG_G Cuts at: 513 1065 2004 2735 513 Size: 552 939 731 3074 Fragments arranged by size: 3074 939 731 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3323 3340 3493 3678 4123 325 Size: 1255 1743 17 153 185 445 1498 Fragments arranged by size: 1743 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3542 5254 96 Size: 274 80 33 51 1443 1565 1712 138 Fragments arranged by size: 1712 1565 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2294 2326 5256 5288 2294 Size: 32 2930 32 2302 Fragments arranged by size: 2930 2302 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5296 XbaI T'CTAG_A Cuts at: 2632 2632 Size: 5296 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2389 Size: 5296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5296 XmaI C'CCGG_G Cuts at: 1870 2621 1870 Size: 751 4545 XmnI GAAnn'nnTTC Cuts at: 3425 3425 Size: 5296 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3104 276 Size: 53 83 186 936 1570 2468 Fragments arranged by size: 2468 1570 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI