(Circular) (Six-base) MAPSORT of: pFN21AB7399.seq Check: 4497 from: 1 to: 5233 pFN21AB7399 5233 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3043 278 Size: 53 83 186 936 1507 2468 Fragments arranged by size: 2468 1507 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2576 1937 Size: 639 4594 Acc65I G'GTAC_C Cuts at: 2554 2554 Size: 5233 AclI AA'CG_TT Cuts at: 1563 3360 3733 1563 Size: 1797 373 3063 Fragments arranged by size: 3063 1797 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2368 3296 4680 4948 1632 Size: 736 928 1384 268 1917 Fragments arranged by size: 1917 1384 928 736 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5036 AflIII A'CryG_T Cuts at: 1203 4132 5109 1203 Size: 2929 977 1327 Fragments arranged by size: 2929 1327 977 AgeI A'CCGG_T Cuts at: 4064 4064 Size: 5233 AhdI GACnn_n'nnGTC Cuts at: 3962 3962 Size: 5233 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5233 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5233 AlwNI CAG_nnn'CTG Cuts at: 2976 4548 2976 Size: 1572 3661 ApaLI G'TGCA_C Cuts at: 3290 4446 3290 Size: 1156 4077 ApoI r'AATT_y Cuts at: 1493 2542 2768 2920 5029 1493 Size: 1049 226 152 2109 1697 Fragments arranged by size: 2109 1697 1049 226 152 AseI AT'TA_AT Cuts at: 160 1032 3787 160 Size: 872 2755 1606 Fragments arranged by size: 2755 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5233 AvaI C'yCGr_G Cuts at: 1870 1943 2558 1870 Size: 73 615 4545 Fragments arranged by size: 4545 615 73 BamHI G'GATC_C Cuts at: 2563 2930 2563 Size: 367 4866 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2554 4009 618 Size: 325 217 627 767 1455 1842 Fragments arranged by size: 1842 1455 767 627 325 217 BanII G_rGCy'C Cuts at: 1391 2510 2552 1391 Size: 1119 42 4072 Fragments arranged by size: 4072 1119 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2170 2285 928 Size: 971 271 115 3876 Fragments arranged by size: 3876 971 271 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3424 3458 1903 Size: 34 1487 34 3678 Fragments arranged by size: 3678 1487 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3125 4335 3125 Size: 1210 4023 BclI T'GATC_A Cuts at: 1548 1548 Size: 5233 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3844 136 Size: 107 122 71 631 605 2172 1525 Fragments arranged by size: 2172 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5228 5228 Size: 5233 BlpI GC'TnA_GC Cuts at: 2021 2033 2650 2021 Size: 12 617 4604 Fragments arranged by size: 4604 617 12 Bme1580I G_kGCm'C Cuts at: 1790 3294 4450 1790 Size: 1504 1156 2573 Fragments arranged by size: 2573 1504 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5233 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2964 3922 442 Size: 416 53 83 399 24 1547 958 1753 Fragments arranged by size: 1753 1547 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5233 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 3893 1239 Size: 1121 1533 2579 Fragments arranged by size: 2579 1533 1121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3358 4223 4521 3358 Size: 865 298 4070 Fragments arranged by size: 4070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2160 2393 3896 882 Size: 617 661 233 1503 2219 Fragments arranged by size: 2219 1503 661 617 233 BsaAI yAC'GTr Cuts at: 493 1204 3024 493 Size: 711 1820 2702 Fragments arranged by size: 2702 1820 711 BsaBI GATnn'nnATC Cuts at: 5227 5227 Size: 5233 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3040 3422 4057 275 Size: 53 83 186 936 1507 382 635 1451 Fragments arranged by size: 1507 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2184 3666 4064 4338 4485 4896 2184 Size: 1482 398 274 147 411 2521 Fragments arranged by size: 2521 1482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5008 Fragments arranged by size: 5008 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5233 BseYI C'CCAG_C Cuts at: 4436 4932 4436 Size: 496 4737 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 1163 Size: 573 525 4135 Fragments arranged by size: 4135 573 525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2683 3444 4472 1540 Size: 275 186 682 761 1028 2301 Fragments arranged by size: 2301 1028 761 682 275 186 BsiHKAI G_wGCw'C Cuts at: 3294 3379 4450 3294 Size: 85 1071 4077 Fragments arranged by size: 4077 1071 85 BsmI GAATG_Cn' Cuts at: 2744 2837 2744 Size: 93 5140 BsmBI CGTCTCn'nnnn_ Cuts at: 2341 2341 Size: 5233 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2510 2552 3294 3379 4450 1391 Size: 399 720 42 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 720 399 85 42 BspEI T'CCGG_A Cuts at: 2184 4896 2184 Size: 2712 2521 BspHI T'CATG_A Cuts at: 3122 4852 3122 Size: 1730 3503 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2435 2588 844 Size: 1591 153 3489 Fragments arranged by size: 3489 1591 153 BsrBI CCG'CTC Cuts at: 1394 1823 3120 1394 Size: 429 1297 3507 Fragments arranged by size: 3507 1297 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3728 3902 65 Size: 1183 2480 174 1396 Fragments arranged by size: 2480 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3877 4064 1700 Size: 110 2067 187 2869 Fragments arranged by size: 2869 2067 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5233 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5233 BssSI C'ACGA_G Cuts at: 3293 4305 3293 Size: 1012 4221 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5233 BstBI TT'CG_AA Cuts at: 2229 4037 2229 Size: 1808 3425 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5233 BstYI r'GATC_y Cuts at: 1972 2479 2563 2930 3315 3332 4784 4870 4882 Size: 507 84 367 385 17 1452 86 12 Cuts at: 4882 5228 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 507 385 367 346 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2171 2171 Size: 5233 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3742 Fragments arranged by size: 3742 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2026 487 Size: 683 56 800 3694 Fragments arranged by size: 3694 800 683 56 BtsI GCAGTG_nn' Cuts at: 2749 3543 3563 2749 Size: 794 20 4419 Fragments arranged by size: 4419 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5233 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5198 DraI TTT'AAA Cuts at: 2537 2537 Size: 5233 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2174 4240 809 Size: 507 858 2066 1802 Fragments arranged by size: 2066 1802 858 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2331 2680 Size: 54 1425 50 275 18 264 237 349 Cuts at: 2680 3569 5198 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 349 275 264 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2680 1537 Size: 275 868 4090 Fragments arranged by size: 4090 868 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3163 1353 Size: 1810 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3820 4194 4340 387 Size: 1064 138 2231 374 146 1280 Fragments arranged by size: 2231 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2368 3296 3893 4680 4948 1239 Size: 393 728 8 928 597 787 268 1524 Fragments arranged by size: 1524 928 787 728 597 393 268 8 EcoRI G'AATT_C Cuts at: 2542 2920 2542 Size: 378 4855 FspI TGC'GCA Cuts at: 3739 3739 Size: 5233 HaeII r_GCGC'y Cuts at: 1678 2417 4380 1678 Size: 739 1963 2531 Fragments arranged by size: 2531 1963 739 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3874 3906 3948 3980 1373 Size: 32 135 32 2302 32 42 32 2626 Fragments arranged by size: 2626 2302 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2577 2823 669 Size: 988 281 639 246 3079 Fragments arranged by size: 3079 988 639 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5233 HpaI GTT'AAC Cuts at: 2823 2823 Size: 5233 KpnI G_GTAC'C Cuts at: 2558 2558 Size: 5233 MfeI C'AATT_G Cuts at: 2832 2832 Size: 5233 MluI A'CGCG_T Cuts at: 5109 5109 Size: 5233 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4347 4531 1305 Size: 3042 184 2007 Fragments arranged by size: 3042 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 10 Size: 54 1425 343 264 3147 Fragments arranged by size: 3147 1425 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 3191 3550 3709 518 Size: 648 906 1119 359 159 2042 Fragments arranged by size: 2042 1119 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2649 3326 4474 4719 1606 Size: 1043 677 1148 245 2120 Fragments arranged by size: 2120 1148 1043 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3742 Fragments arranged by size: 3742 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5233 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5233 NotI GC'GGCC_GC Cuts at: 2680 2680 Size: 5233 NspI r_CATG'y Cuts at: 1132 1229 2591 4136 1132 Size: 97 1362 1545 2229 Fragments arranged by size: 2229 1545 1362 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5233 PciI A'CATG_T Cuts at: 4132 4132 Size: 5233 PflMI CCAn_nnn'nTGG Cuts at: 1108 2410 2529 1108 Size: 1302 119 3812 Fragments arranged by size: 3812 1302 119 PmeI GTTT'AAAC Cuts at: 2537 2537 Size: 5233 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3302 3334 4815 4847 3302 Size: 32 1481 32 3688 Fragments arranged by size: 3688 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5233 PsiI TTA'TAA Cuts at: 2803 2803 Size: 5233 PstI C_TGCA'G Cuts at: 830 2585 830 Size: 1755 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5233 SalI G'TCGA_C Cuts at: 1936 2575 1936 Size: 639 4594 SbfI CC_TGCA'GG Cuts at: 2585 2585 Size: 5233 ScaI AGT'ACT Cuts at: 3481 5193 3481 Size: 1712 3521 SfcI C'TryA_G Cuts at: 826 1045 2350 2581 3716 4397 4588 826 Size: 219 1305 231 1135 681 191 1471 Fragments arranged by size: 1471 1305 1135 681 231 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5233 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5233 SmaI CCC'GGG Cuts at: 1872 2560 1872 Size: 688 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3337 4238 4500 820 Size: 197 926 1394 901 262 1553 Fragments arranged by size: 1553 1394 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3024 493 Size: 2531 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5233 SphI G_CATG'C Cuts at: 1132 2591 1132 Size: 1459 3774 SspI AAT'ATT Cuts at: 5 52 2191 2220 3157 5 Size: 47 2139 29 937 2081 Fragments arranged by size: 2139 2081 937 47 29 StuI AGG'CCT Cuts at: 1517 2518 1517 Size: 1001 4232 StyI C'CwwG_G Cuts at: 513 1065 2004 2672 513 Size: 552 939 668 3074 Fragments arranged by size: 3074 939 668 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2411 3260 3277 3430 3615 4060 325 Size: 1255 831 849 17 153 185 445 1498 Fragments arranged by size: 1498 1255 849 831 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3479 5191 96 Size: 274 80 33 51 1443 1502 1712 138 Fragments arranged by size: 1712 1502 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5193 5225 5193 Size: 32 5201 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5233 XbaI T'CTAG_A Cuts at: 2569 2569 Size: 5233 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5233 XmaI C'CCGG_G Cuts at: 1870 2558 1870 Size: 688 4545 XmnI GAAnn'nnTTC Cuts at: 3362 3362 Size: 5233 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3041 276 Size: 53 83 186 936 1507 2468 Fragments arranged by size: 2468 1507 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI