(Circular) (Six-base) MAPSORT of: pFN21AB7398.seq Check: 3131 from: 1 to: 5680 pFN21AB7398 5680 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2278 2278 Size: 5680 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 2633 2744 3001 2633 Size: 111 257 5312 Fragments arranged by size: 5312 257 111 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 2421 3743 5127 5395 1632 Size: 623 166 1322 1384 268 1917 Fragments arranged by size: 1917 1384 1322 623 268 166 AfeI AGC'GCT Cuts at: 2394 2394 Size: 5680 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 4579 5556 1203 Size: 3376 977 1327 Fragments arranged by size: 3376 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 2206 2524 4409 2206 Size: 318 1885 3477 Fragments arranged by size: 3477 1885 318 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2044 1184 Size: 860 4820 AlwNI CAG_nnn'CTG Cuts at: 3423 4995 3423 Size: 1572 4108 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 2763 2796 2829 4234 160 Size: 872 1731 33 33 1405 1606 Fragments arranged by size: 1731 1606 1405 872 33 33 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 3005 1870 Size: 73 1062 4545 Fragments arranged by size: 4545 1062 73 AvrII C'CTAG_G Cuts at: 2204 2963 2204 Size: 759 4921 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2860 2893 2860 Size: 33 5647 BamHI G'GATC_C Cuts at: 2344 3010 3377 2344 Size: 666 367 4647 Fragments arranged by size: 4647 666 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2633 2744 3001 4456 618 Size: 325 217 627 846 111 257 1455 1842 Fragments arranged by size: 1842 1455 846 627 325 257 217 111 BanII G_rGCy'C Cuts at: 1391 2999 1391 Size: 1608 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2051 2460 928 Size: 971 152 409 4148 Fragments arranged by size: 4148 971 409 152 BbvCI CC'TCA_GC Cuts at: 2139 2373 2139 Size: 234 5446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2680 2714 3871 3905 1903 Size: 34 50 34 659 34 1157 34 3678 Fragments arranged by size: 3678 1157 659 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2781 2814 2847 3572 4782 2781 Size: 33 33 725 1210 3679 Fragments arranged by size: 3679 1210 725 33 33 BclI T'GATC_A Cuts at: 1548 2620 1548 Size: 1072 4608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2916 4291 136 Size: 107 122 71 631 605 1244 1375 1525 Fragments arranged by size: 1525 1375 1244 631 605 122 107 71 BglII A'GATC_T Cuts at: 5675 5675 Size: 5680 BlpI GC'TnA_GC Cuts at: 2240 3097 2240 Size: 857 4823 Bme1580I G_kGCm'C Cuts at: 1790 3741 4897 1790 Size: 1951 1156 2573 Fragments arranged by size: 2573 1951 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2564 2640 3411 Size: 416 53 83 399 24 1147 76 771 Cuts at: 3411 4369 442 Size: 958 1753 Fragments arranged by size: 1753 1147 958 771 416 399 83 76 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2049 2316 2049 Size: 267 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2404 2919 4340 1239 Size: 1165 515 1421 2579 Fragments arranged by size: 2579 1421 1165 515 Bpu10I CC'TnA_GC Cuts at: 2139 2373 2139 Size: 234 5446 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3805 4670 4968 3805 Size: 865 298 4517 Fragments arranged by size: 4517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2601 4343 882 Size: 617 1102 1742 2219 Fragments arranged by size: 2219 1742 1102 617 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4113 4511 4785 4932 5343 4113 Size: 398 274 147 411 4450 Fragments arranged by size: 4450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2077 2107 1178 Size: 30 165 30 674 30 4751 Fragments arranged by size: 4751 674 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2025 2117 2498 1186 Size: 839 92 381 4368 Fragments arranged by size: 4368 839 381 92 BseYI C'CCAG_C Cuts at: 2751 2784 2817 2850 2935 4883 5379 2751 Size: 33 33 33 85 1948 496 3052 Fragments arranged by size: 3052 1948 496 85 33 33 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 1163 Size: 573 573 4534 Fragments arranged by size: 4534 573 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3741 3826 4897 3741 Size: 85 1071 4524 Fragments arranged by size: 4524 1071 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2999 3741 3826 4897 1391 Size: 399 1209 742 85 1071 2174 Fragments arranged by size: 2174 1209 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5343 5343 Size: 5680 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2278 3035 844 Size: 1434 757 3489 Fragments arranged by size: 3489 1434 757 BsrBI CCG'CTC Cuts at: 1394 1823 2195 3567 1394 Size: 429 372 1372 3507 Fragments arranged by size: 3507 1372 429 372 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4349 65 Size: 1183 2927 174 1396 Fragments arranged by size: 2927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4324 4511 1700 Size: 110 2514 187 2869 Fragments arranged by size: 2869 2514 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5680 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2344 3010 3377 3762 3779 5231 5317 5329 Size: 372 666 367 385 17 1452 86 12 Cuts at: 5329 5675 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 666 385 372 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2340 2709 2340 Size: 369 5311 BtgI C'CryG_G Cuts at: 513 1065 2322 513 Size: 552 1257 3871 Fragments arranged by size: 3871 1257 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4941 Fragments arranged by size: 4941 683 56 BtsI GCAGTG_nn' Cuts at: 2292 2452 2978 3196 3990 4010 2292 Size: 160 526 218 794 20 3962 Fragments arranged by size: 3962 794 526 218 160 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3127 4016 5645 Size: 54 1425 50 275 18 1297 889 1629 Cuts at: 5645 8 Size: 43 Fragments arranged by size: 1629 1425 1297 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2926 3610 1353 Size: 1573 684 3423 Fragments arranged by size: 3423 1573 684 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2897 4267 4641 4787 387 Size: 1064 138 1308 1370 374 146 1280 Fragments arranged by size: 1370 1308 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2404 2421 2919 3743 4340 5127 Size: 393 623 149 17 498 824 597 787 Cuts at: 5127 5395 1239 Size: 268 1524 Fragments arranged by size: 1524 824 787 623 597 498 393 268 149 17 EcoNI CCTnn'n_nnAGG Cuts at: 2274 2513 2274 Size: 239 5441 EcoO109I rG'GnC_Cy Cuts at: 2669 2669 Size: 5680 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2396 4827 1678 Size: 718 2431 2531 Fragments arranged by size: 2531 2431 718 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2510 2542 4321 Size: 32 135 32 620 32 286 32 1779 Cuts at: 4321 4353 4395 4427 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1779 620 286 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 2637 2748 3005 2637 Size: 111 257 5312 Fragments arranged by size: 5312 257 111 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4794 4978 1305 Size: 3489 184 2007 Fragments arranged by size: 3489 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3858 Fragments arranged by size: 3858 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3638 3997 4156 518 Size: 648 2472 359 159 2042 Fragments arranged by size: 2472 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2751 2784 2817 2850 2939 3096 3773 4921 Size: 1145 33 33 33 89 157 677 1148 Cuts at: 4921 5166 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1145 677 245 157 89 33 33 33 NcoI C'CATG_G Cuts at: 513 1065 2322 513 Size: 552 1257 3871 Fragments arranged by size: 3871 1257 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 3038 4583 1132 Size: 97 1809 1545 2229 Fragments arranged by size: 2229 1809 1545 97 PasI CC'CwG_GG Cuts at: 1348 2628 2672 1348 Size: 1280 44 4356 Fragments arranged by size: 4356 1280 44 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 2122 1108 Size: 1014 4666 PfoI T'CCnGG_A Cuts at: 2773 2806 2839 2896 2773 Size: 33 33 57 5557 Fragments arranged by size: 5557 57 33 33 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 2275 2287 3032 830 Size: 1445 12 745 3478 Fragments arranged by size: 3478 1445 745 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2751 2784 2817 2850 2939 2751 Size: 33 33 33 89 5492 Fragments arranged by size: 5492 89 33 33 33 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SapI GCTCTTCn'nnn_ Cuts at: 2926 2926 Size: 5680 SbfI CC_TGCA'GG Cuts at: 2275 3032 2275 Size: 757 4923 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 2271 2283 3028 4163 4844 5035 826 Size: 219 1226 12 745 1135 681 191 1471 Fragments arranged by size: 1471 1226 1135 745 681 219 191 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3784 4685 4947 820 Size: 197 926 1841 901 262 1553 Fragments arranged by size: 1841 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 2761 2794 2827 2860 3604 5 Size: 47 2709 33 33 33 744 2081 Fragments arranged by size: 2709 2081 744 47 33 33 33 StuI AGG'CCT Cuts at: 1517 2073 2487 1517 Size: 556 414 4710 Fragments arranged by size: 4710 556 414 StyI C'CwwG_G Cuts at: 513 1065 2204 2322 2348 2496 2963 3119 513 Size: 552 1139 118 26 148 467 156 3074 Fragments arranged by size: 3074 1139 552 467 156 148 118 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2509 3707 3724 3877 4062 4507 325 Size: 1255 929 1198 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1198 929 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3926 5638 96 Size: 274 80 33 51 1443 1949 1712 138 Fragments arranged by size: 1949 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2077 2109 5640 5672 2077 Size: 32 3531 32 2085 Fragments arranged by size: 3531 2085 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2748 2781 2814 2847 2748 Size: 33 33 33 5581 Fragments arranged by size: 5581 33 33 33 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 3809 3809 Size: 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI