Restriction map for
pFN21AB7396
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# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 360 GTAC GT^AC
AflIII 92 ACATGT A^C[GA][CT]GT
AgeI 205 ACCGGT A^CCGGT
AlwI 499 GATCC GGATC(4/5)
AvaII 566 GGTCC G^G[AT]CC
BbsI 484 GAAGAC GAAGAC(2/6)
BcnI 530 CCGGG CC^[GC]GG
BglI 529 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 443 CTGGAG CTGGAG(16/14)
BpuAI 484 GAAGAC GAAGAC(2/6)
BsaWI 205 ACCGGT [AT]^CCGG[AT]
BseRI 520 GAGGAG GAGGAG(10/8)
BspLU11I 92 ACATGT A^CATGT
BspMI 218 ACCTGC ACCTGC(4/8)
BsrBI 149 GAGCGG CCGCTC(-3/-3)
BsrFI 205 ACCGGT [GA]^CCGG[CT]
Cfr10I 205 ACCGGT [GA]^CCGG[CT]
DdeI 413 CTCAG C^T[ACGT]AG
DpnII 499 GATC ^GATC
DraII 323 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 109 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 517 GAAGAG CTCTTC(1/4)
EcoO109I 323 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 291 GCGGG CCCGC(4/6)
HaeIII 324 GGCC GG^CC
HindIII 556 AAGCTT A^AGCTT
HphI 196 GGTGA GGTGA(8/7)
Ksp632I 517 GAAGAG CTCTTC(1/4)
MboI 499 GATC ^GATC
NciI 530 CCGGG CC^[GC]GG
NdeII 499 GATC ^GATC
PflMI 502 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 205 ACCGGT A^CCGGT
RsaI 360 GTAC GT^AC
Sau3AI 499 GATC ^GATC
SfaNI 526 GATGC GCATC(5/9)
SgrAI 204 CACCGGTG C[GA]^CCGG[CT]G
SphI 211 GCATGC GCATG^C
TaqI 343 TCGA T^CGA
TspRI 259 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 343 TCGA T^CGA
Van91I 502 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 151 GCGG CCGC(-3/-1)
291 GCGG
AcsI 75 AAATTT [GA]^AATT[CT]
378 AAATTC
ApoI 75 AAATTT [GA]^AATT[CT]
378 AAATTC
BanI 202 GGCACC G^G[CT][GA]CC
263 GGCACC
BsmBI 141 GAGACG CGTCTC(1/5)
446 GAGACG
BsmFI 293 GGGAC GGGAC(10/14)
572 GTCCC
Cfr13I 324 GGCCC G^G[ACGT]CC
566 GGTCC
HapII 206 CCGG C^CGG
530 CCGG
HpaII 206 CCGG C^CGG
530 CCGG
MaeII 144 ACGT A^CGT
389 ACGT
MspI 206 CCGG C^CGG
530 CCGG
MwoI 529 GCCGGGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
534 GCTGGCAGGGC
NlaIV 202 GGCACC GG[ACGT]^[ACGT]CC
263 GGCACC
NspI 92 ACATGT [GA]CATG^[CT]
211 GCATGC
Sau96I 324 GGCCC G^G[ACGT]CC
566 GGTCC
Tsp509I 76 AATT ^AATT
379 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I EcoRII Fnu4HI FokI ItaI
MboII MnlI MvaI NlaIII ScrFI
TseI