Restriction map for
pFN21AB7393
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BcgI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HaeII HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MluI MluNI MroI
MscI MspA1I NarI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RcaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 166 CGCG CG^CG
AcyI 73 GGCGTC G[GA]^CG[CT]C
AfaI 1172 GTAC GT^AC
AflII 751 CTTAAG C^TTAAG
AlwI 897 GGATC GGATC(4/5)
AocI 534 CCTTAGG CC^T[ACGT]AGG
BbsI 378 GAAGAC GAAGAC(2/6)
BbvCI 696 CCTCAGC CCTCAGC(-5/-2)
BciVI 1053 GGATAC GTATCC(6/5)
BclI 26 TGATCA T^GATCA
BcnI 63 CCGGG CC^[GC]GG
BfrI 751 CTTAAG C^TTAAG
BpuAI 378 GAAGAC GAAGAC(2/6)
BsaHI 73 GGCGTC G[GA]^CG[CT]C
BsaI 590 GAGACC GGTCTC(1/5)
BsmFI 169 GGGAC GGGAC(10/14)
BsmI 1128 GCATTC GAATGC(1/-1)
BstF5I 422 CATCC GGATG(2/0)
BstUI 166 CGCG CG^CG
BstYI 897 GGATCT [GA]^GATC[CT]
Bsu36I 534 CCTTAGG CC^T[ACGT]AGG
CfoI 165 GCGC GCG^C
CpoI 125 CGGTCCG CG^G[AT]CCG
DraII 169 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1141 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 534 CCTTAGG CC^T[ACGT]AGG
EcoO109I 169 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 265 GATATC GAT^ATC
FbaI 26 TGATCA T^GATCA
FokI 422 CATCC GGATG(9/13)
HaeIII 282 GGCC GG^CC
HgaI 74 GCGTC GACGC(5/10)
HhaI 165 GCGC GCG^C
Hin1I 73 GGCGTC G[GA]^CG[CT]C
HinP1I 165 GCGC G^CGC
MfeI 584 CAATTG C^AATTG
MflI 897 GGATCT [GA]^GATC[CT]
MslI 369 CACACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 584 CAATTG C^AATTG
MvnI 166 CGCG CG^CG
NaeI 32 GCCGGC GCC^GGC
NciI 63 CCGGG CC^[GC]GG
NdeI 1010 CATATG CA^TATG
NgoMIV 32 GCCGGC G^CCGGC
NlaIII 1119 CATG CATG^
PpuMI 169 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 547 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1172 GTAC GT^AC
RsrII 125 CGGTCCG CG^G[AT]CCG
TaqI 840 TCGA T^CGA
TthHB8I 840 TCGA T^CGA
XhoII 897 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BbvI 835 GCAGC GCAGC(8/12)
948 GCTGC
BfaI 863 CTAG C^TAG
1207 CTAG
BpmI 670 CTCCAG CTGGAG(16/14)
742 CTCCAG
BsgI 980 CTGCAC GTGCAG(16/14)
727 GTGCAG
BsmAI 321 GAGAC GTCTC(1/5)
590 GAGAC
BsrFI 32 GCCGGC [GA]^CCGG[CT]
123 GCCGGT
BstNI 110 CCAGG CC^[AT]GG
744 CCAGG
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
123 GCCGGT
DpnII 27 GATC ^GATC
898 GATC
EcoRII 110 CCAGG ^CC[AT]GG
744 CCAGG
EcoT14I 47 CCTTGG C^C[AT][AT]GG
464 CCTTGG
HindIII 571 AAGCTT A^AGCTT
847 AAGCTT
HphI 24 GGTGA GGTGA(8/7)
209 TCACC
MaeI 863 CTAG C^TAG
1207 CTAG
MaeIII 554 GTCAC ^GT[ACGT]AC
678 GTCAC
MboI 27 GATC ^GATC
898 GATC
MvaI 110 CCAGG CC^[AT]GG
744 CCAGG
MwoI 167 GCGGGACCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
524 GCTTAAAAAGC
NdeII 27 GATC ^GATC
898 GATC
PflMI 1044 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCACACTGTGG
Sau3AI 27 GATC ^GATC
898 GATC
SmlI 549 CTTGAG C^T[CT][GA]AG
751 CTTAAG
StyI 47 CCTTGG C^C[AT][AT]GG
464 CCTTGG
TseI 835 GCAGC G^C[AT]GC
948 GCTGC
Tsp45I 554 GTCAC ^GT[GC]AC
678 GTCAC
Van91I 1044 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCACACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
AvaII BsaJI BsiYI BslI BsrI
BssKI Cac8I Cfr13I DdeI DraI
FauI Fnu4HI HapII HinfI HpaII
ItaI MboII MnlI MseI MspI
NlaIV PleI Sau96I ScrFI TfiI
Tru9I Tsp509I TspRI XmnI