(Circular) (Six-base) MAPSORT of: pFN21AB7393.seq Check: 3772 from: 1 to: 5947 pFN21AB7393 5947 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3290 1937 Size: 1353 4594 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2180 2373 2465 3201 4010 5394 5662 1632 Size: 548 193 92 736 809 1384 268 1917 Fragments arranged by size: 1917 1384 809 736 548 268 193 92 AflII C'TTAA_G Cuts at: 820 1017 2756 820 Size: 197 1739 4011 Fragments arranged by size: 4011 1739 197 AflIII A'CryG_T Cuts at: 1203 4846 5823 1203 Size: 3643 977 1327 Fragments arranged by size: 3643 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 2378 1166 Size: 1212 4735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3156 1184 Size: 1972 3975 AlwNI CAG_nnn'CTG Cuts at: 3690 5262 3690 Size: 1572 4375 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 2219 2393 2455 2761 2873 2881 3069 3256 Size: 726 174 62 306 112 8 188 187 Cuts at: 3256 3482 3634 5743 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 726 306 226 188 187 174 152 112 62 8 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2346 2379 2346 Size: 33 5914 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3268 4723 618 Size: 325 217 627 1481 1455 1842 Fragments arranged by size: 1842 1481 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3266 1391 Size: 1875 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2390 928 Size: 971 491 4485 Fragments arranged by size: 4485 971 491 BbvCI CC'TCA_GC Cuts at: 2702 2702 Size: 5947 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4138 4172 1903 Size: 34 2201 34 3678 Fragments arranged by size: 3678 2201 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3052 3839 5049 3052 Size: 787 1210 3950 Fragments arranged by size: 3950 1210 787 BclI T'GATC_A Cuts at: 1548 2031 1548 Size: 483 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4558 136 Size: 107 122 71 631 605 2886 1525 Fragments arranged by size: 2886 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 4008 5164 1790 Size: 2218 1156 2573 Fragments arranged by size: 2573 2218 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3678 4636 442 Size: 416 53 83 399 24 2261 958 1753 Fragments arranged by size: 2261 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2349 2349 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2660 2732 4607 1239 Size: 1421 72 1875 2579 Fragments arranged by size: 2579 1875 1421 72 Bpu10I CC'TnA_GC Cuts at: 2060 2189 2702 2060 Size: 129 513 5305 Fragments arranged by size: 5305 513 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2575 4072 4937 5235 2575 Size: 1497 865 298 3287 Fragments arranged by size: 3287 1497 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2589 4610 882 Size: 617 1090 2021 2219 Fragments arranged by size: 2219 2021 1090 617 BsaAI yAC'GTr Cuts at: 493 1204 3738 493 Size: 711 2534 2702 Fragments arranged by size: 2702 2534 711 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 3754 4136 4771 Size: 53 83 186 936 546 1675 382 635 Cuts at: 4771 275 Size: 1451 Fragments arranged by size: 1675 1451 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4380 4778 5052 5199 5610 4380 Size: 398 274 147 411 4717 Fragments arranged by size: 4717 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5722 Fragments arranged by size: 5722 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5947 BseYI C'CCAG_C Cuts at: 5150 5646 5150 Size: 496 5451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2753 2970 1163 Size: 573 1017 217 4140 Fragments arranged by size: 4140 1017 573 217 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4008 4093 5164 4008 Size: 85 1071 4791 Fragments arranged by size: 4791 1071 85 BsmI GAATG_Cn' Cuts at: 3133 3458 3551 3133 Size: 325 93 5529 Fragments arranged by size: 5529 325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3266 4008 4093 5164 1391 Size: 399 1476 742 85 1071 2174 Fragments arranged by size: 2174 1476 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3302 844 Size: 2458 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3834 1394 Size: 429 2011 3507 Fragments arranged by size: 3507 2011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4442 4616 65 Size: 1183 3194 174 1396 Fragments arranged by size: 3194 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 2128 4591 4778 1700 Size: 110 227 91 2463 187 2869 Fragments arranged by size: 2869 2463 227 187 110 91 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5947 BstBI TT'CG_AA Cuts at: 4751 4751 Size: 5947 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstYI r'GATC_y Cuts at: 1972 2902 3277 3644 4029 4046 5498 5584 5596 Size: 930 375 367 385 17 1452 86 12 Cuts at: 5596 5942 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 930 385 375 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2540 2540 Size: 5947 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5395 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5208 Fragments arranged by size: 5208 683 56 BtsI GCAGTG_nn' Cuts at: 3463 4257 4277 3463 Size: 794 20 5133 Fragments arranged by size: 5133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 2304 2407 2766 3251 2304 Size: 103 359 485 5000 Fragments arranged by size: 5000 485 359 103 DraIII CAC_nnn'GTG Cuts at: 3151 3151 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912 Size: 54 1425 50 275 18 1564 889 1629 Cuts at: 5912 8 Size: 43 Fragments arranged by size: 1629 1564 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3877 1353 Size: 2524 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2373 2465 2660 2732 3201 4010 Size: 393 548 193 92 195 72 469 809 Cuts at: 4010 4607 5394 5662 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 809 787 597 548 469 393 268 195 193 92 72 EcoO109I rG'GnC_Cy Cuts at: 2175 2175 Size: 5947 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 EcoRV GAT'ATC Cuts at: 2272 2272 Size: 5947 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2330 2330 Size: 5947 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 5094 1678 Size: 3416 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 4588 4620 4662 Size: 32 135 32 514 32 2470 32 42 Cuts at: 4662 4694 1373 Size: 32 2626 Fragments arranged by size: 2626 2470 514 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3291 3537 669 Size: 988 281 1353 246 3079 Fragments arranged by size: 3079 1353 988 281 246 HindIII A'AGCT_T Cuts at: 748 2576 2852 748 Size: 1828 276 3843 Fragments arranged by size: 3843 1828 276 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 2589 3546 2589 Size: 957 4990 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5061 5245 1305 Size: 3756 184 2007 Fragments arranged by size: 3756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4125 Fragments arranged by size: 4125 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2378 3905 4264 4423 518 Size: 648 1212 1527 359 159 2042 Fragments arranged by size: 2042 1527 1212 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3363 4040 5188 5433 1606 Size: 1757 677 1148 245 2120 Fragments arranged by size: 2120 1757 1148 677 245 NaeI GCC'GGC Cuts at: 2039 2039 Size: 5947 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5395 NdeI CA'TA_TG Cuts at: 387 3016 387 Size: 2629 3318 NgoMIV G'CCGG_C Cuts at: 2037 2037 Size: 5947 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 3305 4850 1132 Size: 97 2076 1545 2229 Fragments arranged by size: 2229 2076 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 3055 3151 1108 Size: 1947 96 3904 Fragments arranged by size: 3904 1947 96 PfoI T'CCnGG_A Cuts at: 2114 2748 2114 Size: 634 5313 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2175 2175 Size: 5947 PshAI GACnn'nnGTC Cuts at: 1613 2556 1613 Size: 943 5004 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PstI C_TGCA'G Cuts at: 830 3299 830 Size: 2469 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 RsrII CG'GwC_CG Cuts at: 2131 2131 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SanDI GG'GwC_CC Cuts at: 2175 2175 Size: 5947 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 3295 4430 5111 5302 826 Size: 219 2250 1135 681 191 1471 Fragments arranged by size: 2250 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2554 2756 4051 4952 5214 820 Size: 197 926 611 202 1295 901 262 1553 Fragments arranged by size: 1553 1295 926 901 611 262 202 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5947 StyI C'CwwG_G Cuts at: 513 1065 2052 2469 3386 513 Size: 552 987 417 917 3074 Fragments arranged by size: 3074 987 917 552 417 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3161 3974 3991 4144 4329 4774 325 Size: 1255 1581 813 17 153 185 445 1498 Fragments arranged by size: 1581 1498 1255 813 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4193 5905 96 Size: 274 80 33 51 1443 2216 1712 138 Fragments arranged by size: 2216 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 2478 2787 3101 4076 2478 Size: 309 314 975 4349 Fragments arranged by size: 4349 975 314 309 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI