(Circular) (Six-base) MAPSORT of: pFN21AB7392.seq Check: 7960 from: 1 to: 5725 pFN21AB7392 5725 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2144 3535 278 Size: 53 83 186 936 608 1391 2468 Fragments arranged by size: 2468 1391 936 608 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3788 5172 5440 1632 Size: 2156 1384 268 1917 Fragments arranged by size: 2156 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 3468 5040 3468 Size: 1572 4153 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 2043 2549 3034 3260 3412 5521 1493 Size: 550 506 485 226 152 2109 1697 Fragments arranged by size: 2109 1697 550 506 485 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2180 2313 2775 3050 1870 Size: 73 237 133 462 275 4545 Fragments arranged by size: 4545 462 275 237 133 73 AvrII C'CTAG_G Cuts at: 2809 2809 Size: 5725 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2863 3046 4501 618 Size: 325 217 627 1076 183 1455 1842 Fragments arranged by size: 1842 1455 1076 627 325 217 183 BanII G_rGCy'C Cuts at: 1391 2116 2295 2709 2914 3044 1391 Size: 725 179 414 205 130 4072 Fragments arranged by size: 4072 725 414 205 179 130 BbeI G_GCGC'C Cuts at: 2867 2867 Size: 5725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2247 2886 928 Size: 971 348 639 3767 Fragments arranged by size: 3767 971 639 348 BbvCI CC'TCA_GC Cuts at: 2874 2874 Size: 5725 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 2100 1548 Size: 552 5173 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 3786 4942 1790 Size: 1996 1156 2573 Fragments arranged by size: 2573 1996 1156 BmgBI CAC'GTC Cuts at: 1318 2098 1318 Size: 780 4945 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3456 4414 442 Size: 416 53 83 399 24 2039 958 1753 Fragments arranged by size: 2039 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2960 4385 1239 Size: 1721 1425 2579 Fragments arranged by size: 2579 1721 1425 Bpu10I CC'TnA_GC Cuts at: 2874 2874 Size: 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2288 3850 4715 5013 2288 Size: 1562 865 298 3000 Fragments arranged by size: 3000 1562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 2864 3532 3914 Size: 53 83 186 936 608 723 668 382 Cuts at: 3914 4549 275 Size: 635 1451 Fragments arranged by size: 1451 936 723 668 635 608 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2665 4158 4556 4830 4977 5388 2665 Size: 1493 398 274 147 411 3002 Fragments arranged by size: 3002 1493 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2958 2988 1178 Size: 30 165 30 1555 30 3915 Fragments arranged by size: 3915 1555 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2222 2225 2228 2231 2303 1186 Size: 1027 9 3 3 3 72 4608 Fragments arranged by size: 4608 1027 72 9 3 3 3 BseYI C'CCAG_C Cuts at: 4928 5424 4928 Size: 496 5229 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2802 1163 Size: 573 1066 4086 Fragments arranged by size: 4086 1066 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2295 3786 3871 4942 2295 Size: 1491 85 1071 3078 Fragments arranged by size: 3078 1491 1071 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2295 2709 2914 3044 3786 3871 Size: 399 326 179 414 205 130 742 85 Cuts at: 3871 4942 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 414 399 326 205 179 130 85 BspEI T'CCGG_A Cuts at: 2665 5388 2665 Size: 2723 3002 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3080 844 Size: 2236 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2320 3612 1394 Size: 429 497 1292 3507 Fragments arranged by size: 3507 1292 497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2952 4369 4556 1700 Size: 110 1142 1417 187 2869 Fragments arranged by size: 2869 1417 1142 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 2285 3785 4797 2285 Size: 1500 1012 3213 Fragments arranged by size: 3213 1500 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstYI r'GATC_y Cuts at: 1972 2813 3055 3422 3807 3824 5276 5362 5374 Size: 841 242 367 385 17 1452 86 12 Cuts at: 5374 5720 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 841 385 367 346 242 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2600 513 Size: 552 1535 3638 Fragments arranged by size: 3638 1535 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 3241 4035 4055 3241 Size: 794 20 4911 Fragments arranged by size: 4911 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2603 3172 4061 Size: 54 1425 50 275 18 773 569 889 Cuts at: 4061 5690 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 773 569 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2190 2199 2217 2223 2716 2942 3655 Size: 749 88 9 18 6 493 226 713 Cuts at: 3655 1353 Size: 3423 Fragments arranged by size: 3423 749 713 493 226 88 18 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2293 2293 Size: 5725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2960 3788 4385 5172 5440 1239 Size: 393 1328 828 597 787 268 1524 Fragments arranged by size: 1524 1328 828 787 597 393 268 EcoRI G'AATT_C Cuts at: 2043 3034 3412 2043 Size: 991 378 4356 Fragments arranged by size: 4356 991 378 EcoRV GAT'ATC Cuts at: 2072 2072 Size: 5725 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2063 2063 Size: 5725 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2867 2874 4872 1678 Size: 1189 7 1998 2531 Fragments arranged by size: 2531 1998 1189 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 2771 2803 3005 Size: 32 135 32 911 32 256 32 202 Cuts at: 3005 3037 4366 4398 4440 4472 1373 Size: 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1329 911 256 202 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KasI G'GCGC_C Cuts at: 2863 2863 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2587 4839 5023 1305 Size: 1282 2252 184 2007 Fragments arranged by size: 2252 2007 1282 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3903 Fragments arranged by size: 3903 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2426 3683 4042 4201 518 Size: 648 1260 1257 359 159 2042 Fragments arranged by size: 2042 1260 1257 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2641 3141 3818 4966 5211 1606 Size: 486 549 500 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 549 500 486 245 NarI GG'CG_CC Cuts at: 2864 2864 Size: 5725 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 3083 4628 1132 Size: 97 1854 1545 2229 Fragments arranged by size: 2229 1854 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2027 2251 1108 Size: 919 224 4582 Fragments arranged by size: 4582 919 224 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 SacI G_AGCT'C Cuts at: 2295 2295 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SapI GCTCTTCn'nnn_ Cuts at: 2942 2942 Size: 5725 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 2620 3973 5685 2620 Size: 1353 1712 2660 Fragments arranged by size: 2660 1712 1353 SfcI C'TryA_G Cuts at: 826 1045 3073 4208 4889 5080 826 Size: 219 2028 1135 681 191 1471 Fragments arranged by size: 2028 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2865 2865 Size: 5725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 2315 3052 1872 Size: 443 737 4545 Fragments arranged by size: 4545 737 443 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 3829 4730 4992 820 Size: 197 926 360 1526 901 262 1553 Fragments arranged by size: 1553 1526 926 901 360 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 2470 3649 5 Size: 47 2418 1179 2081 Fragments arranged by size: 2418 2081 1179 47 StuI AGG'CCT Cuts at: 1517 2760 1517 Size: 1243 4482 StyI C'CwwG_G Cuts at: 513 1065 2809 3164 513 Size: 552 1744 355 3074 Fragments arranged by size: 3074 1744 552 355 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2836 3752 3769 3922 4107 4552 325 Size: 1255 1256 916 17 153 185 445 1498 Fragments arranged by size: 1498 1256 1255 916 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2618 3971 5683 Size: 274 80 33 51 1443 641 1353 1712 Cuts at: 5683 96 Size: 138 Fragments arranged by size: 1712 1443 1353 641 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5685 5717 5685 Size: 32 5693 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 2330 2380 3061 2330 Size: 50 681 4994 Fragments arranged by size: 4994 681 50 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 2313 3050 1870 Size: 443 737 4545 Fragments arranged by size: 4545 737 443 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2142 3533 276 Size: 53 83 186 936 608 1391 2468 Fragments arranged by size: 2468 1391 936 608 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI