Restriction map for
pFN21AB7389
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
BalI BamHI BbeI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlpI Bpu1102I BpuAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 487 GTAGAC GT^[AC][GT]AC
AccII 181 CGCG CG^CG
AlwI 60 GATCC GGATC(4/5)
AlwNI 430 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 609 CCTAGG C^CTAGG
BanI 409 GGTGCC G^G[CT][GA]CC
BbrPI 570 CACGTG CAC^GTG
BcgI 468 CGACCACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 222 CCGGG CC^[GC]GG
BfaI 610 CTAG C^TAG
BlnI 609 CCTAGG C^CTAGG
BsaAI 570 CACGTG [CT]AC^GT[GA]
BsaBI 333 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 506 GTGCAG GTGCAG(16/14)
BstEII 217 GGTAACC G^GT[ACGT]ACC
BstPI 217 GGTAACC G^GT[ACGT]ACC
BstUI 181 CGCG CG^CG
BstXI 281 CCATGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 59 AGATCC [GA]^GATC[CT]
DraI 135 TTTAAA TTT^AAA
EaeI 255 TGGCCG [CT]^GGCC[GA]
EcoO65I 217 GGTAACC G^GT[ACGT]ACC
EcoT14I 609 CCTAGG C^C[AT][AT]GG
HapII 222 CCGG C^CGG
HpaII 222 CCGG C^CGG
HphI 122 TCACC GGTGA(8/7)
MaeI 610 CTAG C^TAG
MamI 333 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 59 AGATCC [GA]^GATC[CT]
MslI 282 CATGATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 517 CAGCTG C[AC]G^C[GT]G
MspI 222 CCGG C^CGG
MvnI 181 CGCG CG^CG
NciI 222 CCGGG CC^[GC]GG
NspI 37 ACATGC [GA]CATG^[CT]
PmaCI 570 CACGTG CAC^GTG
PmlI 570 CACGTG CAC^GTG
PvuII 517 CAGCTG CAG^CTG
SacI 244 GAGCTC GAGCT^C
SapI 246 GCTCTTC GCTCTTC(1/4)
SfaNI 96 GATGC GCATC(5/9)
SfcI 105 CTACAG C^T[GA][CT]AG
StyI 609 CCTAGG C^C[AT][AT]GG
Tsp45I 34 GTGAC ^GT[GC]AC
Tsp509I 84 AATT ^AATT
XhoII 59 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 213 GTAC GT^AC
300 GTAC
AspHI 244 GAGCTC G[AT]GC[AT]^C
567 GAGCAC
BanII 244 GAGCTC G[GA]GC[CT]^C
402 GAGCCC
BpmI 241 CTGGAG CTGGAG(16/14)
553 CTGGAG
BsiHKAI 244 GAGCTC G[AT]GC[AT]^C
567 GAGCAC
DraII 224 GGGACCT [GA]G^G[ACGT]CC[CT]
595 GGGACCT
EarI 247 CTCTTC CTCTTC(1/4)
386 GAAGAG
EcoO109I 224 GGGACCT [GA]G^G[ACGT]CC[CT]
595 GGGACCT
HinfI 153 GACTC G^A[ACGT]TC
513 GACTC
Ksp632I 247 CTCTTC CTCTTC(1/4)
386 GAAGAG
MaeII 112 ACGT A^CGT
571 ACGT
MaeIII 34 GTGAC ^GT[ACGT]AC
218 GTAAC
MseI 136 TTAA T^TAA
344 TTAA
PleI 153 GACTC GAGTC(4/5)
513 GACTC
PpuMI 224 GGGACCT [GA]G^G[AT]CC[CT]
595 GGGACCT
RsaI 213 GTAC GT^AC
300 GTAC
Tru9I 136 TTAA T^TAA
344 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII ItaI MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI