Restriction map for
pFN21AB7388
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstYI
BstZ17I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRV FauI
FseI FspI HaeII HapII HhaI
HinP1I HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MflI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NlaIV NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 318 GACGCC G[GA]^CG[CT]C
AocI 211 CCTGAGG CC^T[ACGT]AGG
BclI 690 TGATCA T^GATCA
BfaI 493 CTAG C^TAG
BpmI 313 CTCCAG CTGGAG(16/14)
BsaHI 318 GACGCC G[GA]^CG[CT]C
BseRI 201 CTCCTC GAGGAG(10/8)
BsgI 622 CTGCAC GTGCAG(16/14)
BsmBI 310 CGTCTC CGTCTC(1/5)
BsmI 599 GCATTC GAATGC(1/-1)
BsrI 697 CCAGT ACTGG(1/-1)
BssKI 288 CCTGG ^CC[ACGT]GG
BstEII 891 GGTGACC G^GT[ACGT]ACC
BstNI 288 CCTGG CC^[AT]GG
BstPI 891 GGTGACC G^GT[ACGT]ACC
BstXI 143 CCACAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 211 CCTGAGG CC^T[ACGT]AGG
Cac8I 492 GCTAGC GC[ACGT]^[ACGT]GC
DraI 36 TTTAAA TTT^AAA
DsaI 500 CCATGG C^C[GA][CT]GG
Eco81I 211 CCTGAGG CC^T[ACGT]AGG
EcoO65I 891 GGTGACC G^GT[ACGT]ACC
EcoRI 181 GAATTC G^AATTC
EcoRII 288 CCTGG ^CC[AT]GG
EcoT22I 786 ATGCAT ATGCA^T
FbaI 690 TGATCA T^GATCA
HgaI 318 GACGC GACGC(5/10)
Hin1I 318 GACGCC G[GA]^CG[CT]C
HincII 355 GTTGAC GT[CT]^[GA]AC
HindII 355 GTTGAC GT[CT]^[GA]AC
MaeI 493 CTAG C^TAG
MspA1I 839 CAGCTG C[AC]G^C[GT]G
MvaI 288 CCTGG CC^[AT]GG
MwoI 496 GCAGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 500 CCATGG C^CATGG
NdeI 525 CATATG CA^TATG
NheI 492 GCTAGC G^CTAGC
NsiI 786 ATGCAT ATGCA^T
PshAI 304 GACACTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 839 CAGCTG CAG^CTG
ScrFI 288 CCTGG CC^[ACGT]GG
SfcI 1014 CTATAG C^T[GA][CT]AG
TaqI 207 TCGA T^CGA
TthHB8I 207 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwI 216 GGATC GGATC(4/5)
957 GGATC
BbvI 496 GCAGC GCAGC(8/12)
841 GCTGC
BsaJI 192 CCTTGG C^C[ACGT][ACGT]GG
500 CCATGG
BsiYI 513 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
576 CCTGCTTCTGG
BslI 513 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
576 CCTGCTTCTGG
BsmAI 412 GAGAC GTCTC(1/5)
311 GTCTC
BspMI 275 ACCTGC ACCTGC(4/8)
488 ACCTGC
BsrBI 226 CCGCTC CCGCTC(-3/-3)
297 CCGCTC
EcoT14I 192 CCTTGG C^C[AT][AT]GG
500 CCATGG
MseI 37 TTAA T^TAA
805 TTAA
StyI 192 CCTTGG C^C[AT][AT]GG
500 CCATGG
Tru9I 37 TTAA T^TAA
805 TTAA
TseI 496 GCAGC G^C[AT]GC
841 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BalI BstF5I DdeI DpnII
EaeI Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MluNI MnlI MscI MslI NdeII
NlaIII PleI RsaI Sau3AI SfaNI
TfiI Tsp45I Tsp509I TspRI