Restriction map for
pFN21AB7388
(Linear) (Six-base) MAPSORT of: KIBB7388.seq Check: 1489 from: 1 to: 1080
KIBB7388.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 847 1080
Size: 821 26 233
Fragments arranged by size:
821 233 26
AleI CACnn'nnGTG
Cuts at: 0 889 1080
Size: 889 191
AlwNI CAG_nnn'CTG
Cuts at: 0 569 844 936 1080
Size: 569 275 92 144
Fragments arranged by size:
569 275 144 92
ApoI r'AATT_y
Cuts at: 0 39 75 181 1080
Size: 39 36 106 899
Fragments arranged by size:
899 106 39 36
BclI T'GATC_A
Cuts at: 0 690 1080
Size: 690 390
BfrBI ATG'CAT
Cuts at: 0 788 1080
Size: 788 292
BmtI G_CTAG'C
Cuts at: 0 496 1080
Size: 496 584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1080
Size: 298 782
BsaHI Gr'CG_yC
Cuts at: 0 319 1080
Size: 319 761
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 192 1080
Size: 192 888
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1080
Size: 607 473
BsmI GAATG_Cn'
Cuts at: 0 599 1080
Size: 599 481
BsmBI CGTCTCn'nnnn_
Cuts at: 0 316 1080
Size: 316 764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 284 497 1080
Size: 284 213 583
Fragments arranged by size:
583 284 213
BsrBI CCG'CTC
Cuts at: 0 228 299 1080
Size: 228 71 781
Fragments arranged by size:
781 228 71
BstEII G'GTnAC_C
Cuts at: 0 891 1080
Size: 891 189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 1080
Size: 150 930
Bsu36I CC'TnA_GG
Cuts at: 0 212 1080
Size: 212 868
BtgI C'CryG_G
Cuts at: 0 500 1080
Size: 500 580
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 701 1080
Size: 701 379
DraI TTT'AAA
Cuts at: 0 38 1080
Size: 38 1042
EaeI y'GGCC_r
Cuts at: 0 290 503 521 1080
Size: 290 213 18 559
Fragments arranged by size:
559 290 213 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 821 847 1080
Size: 298 523 26 233
Fragments arranged by size:
523 298 233 26
EcoRI G'AATT_C
Cuts at: 0 181 1080
Size: 181 899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 246 1080
Size: 246 834
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 557 589 1059 1080
Size: 557 32 470 21
Fragments arranged by size:
557 470 32 21
HincII GTy'rAC
Cuts at: 0 357 1080
Size: 357 723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 965 1080
Size: 81 884 115
Fragments arranged by size:
884 115 81
MscI TGG'CCA
Cuts at: 0 292 505 523 1080
Size: 292 213 18 557
Fragments arranged by size:
557 292 213 18
MslI CAynn'nnrTG
Cuts at: 0 148 553 745 889 1080
Size: 148 405 192 144 191
Fragments arranged by size:
405 192 191 148 144
MspA1I CmG'CkG
Cuts at: 0 841 1080
Size: 841 239
NcoI C'CATG_G
Cuts at: 0 500 1080
Size: 500 580
NdeI CA'TA_TG
Cuts at: 0 526 1080
Size: 526 554
NheI G'CTAG_C
Cuts at: 0 492 1080
Size: 492 588
NsiI A_TGCA'T
Cuts at: 0 790 1080
Size: 790 290
PshAI GACnn'nnGTC
Cuts at: 0 308 1080
Size: 308 772
PvuII CAG'CTG
Cuts at: 0 841 1080
Size: 841 239
SfcI C'TryA_G
Cuts at: 0 1014 1080
Size: 1014 66
StyI C'CwwG_G
Cuts at: 0 192 500 1080
Size: 192 308 580
Fragments arranged by size:
580 308 192
TatI w'GTAC_w
Cuts at: 0 326 452 1080
Size: 326 126 628
Fragments arranged by size:
628 326 126
Enzymes that do cut:
AcuI AleI AlwNI ApoI BclI BfrBI BmtI BpmI
BsaHI BseRI BsgI BsmI BsmBI BspMI BsrBI BstEII
BstXI Bsu36I BtgI BtgZI DraI EaeI Eco57MI EcoRI
FalI Hin4I HincII MmeI MscI MslI MspA1I NcoI
NdeI NheI NsiI PshAI PvuII SfcI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BstZ17I BtsI ClaI CspCI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI