(Circular) (Six-base) MAPSORT of: pFN21AB7387.seq Check: 1753 from: 1 to: 5995 pFN21AB7387 5995 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2749 3085 4058 5442 5710 1632 Size: 1117 336 973 1384 268 1917 Fragments arranged by size: 1917 1384 1117 973 336 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 4894 5871 1203 Size: 3691 977 1327 Fragments arranged by size: 3691 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5995 AlwNI CAG_nnn'CTG Cuts at: 2379 3738 5310 2379 Size: 1359 1572 3064 Fragments arranged by size: 3064 1572 1359 ApaLI G'TGCA_C Cuts at: 3195 4052 5208 3195 Size: 857 1156 3982 Fragments arranged by size: 3982 1156 857 ApoI r'AATT_y Cuts at: 1493 2516 2562 2587 2883 3304 3530 3682 5791 Size: 1023 46 25 296 421 226 152 2109 Cuts at: 5791 1493 Size: 1697 Fragments arranged by size: 2109 1697 1023 421 296 226 152 46 25 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 3228 3320 1870 Size: 73 1285 92 4545 Fragments arranged by size: 4545 1285 92 73 AvrII C'CTAG_G Cuts at: 2392 2392 Size: 5995 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2165 2198 2165 Size: 33 5962 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3316 4771 618 Size: 325 217 627 1529 1455 1842 Fragments arranged by size: 1842 1529 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3314 1391 Size: 1923 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5024 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3248 3282 4186 4220 1903 Size: 34 1311 34 904 34 3678 Fragments arranged by size: 3678 1311 904 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2254 2853 3887 5097 2254 Size: 599 1034 1210 3152 Fragments arranged by size: 3152 1210 1034 599 BclI T'GATC_A Cuts at: 1548 2719 1548 Size: 1171 4824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4606 136 Size: 107 122 71 631 605 2934 1525 Fragments arranged by size: 2934 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2045 5990 2045 Size: 3945 2050 BlpI GC'TnA_GC Cuts at: 3412 3412 Size: 5995 Bme1580I G_kGCm'C Cuts at: 1790 3199 4056 5212 1790 Size: 1409 857 1156 2573 Fragments arranged by size: 2573 1409 1156 857 BmgBI CAC'GTC Cuts at: 1318 2143 1318 Size: 825 5170 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2186 2739 3726 Size: 416 53 83 399 24 769 553 987 Cuts at: 3726 4684 442 Size: 958 1753 Fragments arranged by size: 1753 987 958 769 553 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2997 4655 1239 Size: 932 826 1658 2579 Fragments arranged by size: 2579 1658 932 826 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 4120 4985 5283 2525 Size: 1595 865 298 3237 Fragments arranged by size: 3237 1595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4658 882 Size: 617 3159 2219 Fragments arranged by size: 3159 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2653 3786 493 Size: 711 1449 1133 2702 Fragments arranged by size: 2702 1449 1133 711 BsaBI GATnn'nnATC Cuts at: 2265 2331 2418 5989 2265 Size: 66 87 3571 2271 Fragments arranged by size: 3571 2271 87 66 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3802 4184 4819 275 Size: 53 83 186 936 2269 382 635 1451 Fragments arranged by size: 2269 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3059 4428 4826 5100 5247 5658 3059 Size: 1369 398 274 147 411 3396 Fragments arranged by size: 3396 1369 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5770 Fragments arranged by size: 5770 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2111 1186 Size: 838 87 5070 Fragments arranged by size: 5070 838 87 BseYI C'CCAG_C Cuts at: 5198 5694 5198 Size: 496 5499 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 4206 5234 1540 Size: 275 186 1444 761 1028 2301 Fragments arranged by size: 2301 1444 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3199 4056 4141 5212 3199 Size: 857 85 1071 3982 Fragments arranged by size: 3982 1071 857 85 BsmI GAATG_Cn' Cuts at: 2941 3506 3599 2941 Size: 565 93 5337 Fragments arranged by size: 5337 565 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2133 2133 Size: 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3199 3314 4056 4141 5212 1391 Size: 399 1409 115 742 85 1071 2174 Fragments arranged by size: 2174 1409 1071 742 399 115 85 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3350 844 Size: 2506 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3882 1394 Size: 429 2059 3507 Fragments arranged by size: 3507 2059 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2920 4490 4664 65 Size: 1183 1672 1570 174 1396 Fragments arranged by size: 1672 1570 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4639 4826 1700 Size: 110 2829 187 2869 Fragments arranged by size: 2869 2829 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstYI r'GATC_y Cuts at: 1972 2045 3325 3692 4077 4094 5546 5632 5644 Size: 73 1280 367 385 17 1452 86 12 Cuts at: 5644 5990 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1280 385 367 346 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 513 Size: 552 939 75 4429 Fragments arranged by size: 4429 939 552 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 3511 4305 4325 3511 Size: 794 20 5181 Fragments arranged by size: 5181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2902 2937 529 Size: 35 2338 35 3587 Fragments arranged by size: 3587 2338 35 35 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 3442 4331 Size: 54 1425 50 275 18 246 1366 889 Cuts at: 4331 5960 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1366 889 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3925 1353 Size: 2572 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4582 4956 5102 387 Size: 1064 138 2993 374 146 1280 Fragments arranged by size: 2993 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2749 2997 3085 4058 4655 5442 Size: 393 539 578 248 88 973 597 787 Cuts at: 5442 5710 1239 Size: 268 1524 Fragments arranged by size: 1524 973 787 597 578 539 393 268 248 88 EcoRI G'AATT_C Cuts at: 2562 2587 2883 3304 3682 2562 Size: 25 296 421 378 4875 Fragments arranged by size: 4875 421 378 296 25 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 5142 1678 Size: 3464 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2277 2309 2623 2655 2848 Size: 32 135 32 705 32 314 32 193 Cuts at: 2848 2880 3100 3132 4636 4668 4710 4742 1373 Size: 32 220 32 1504 32 42 32 2626 Fragments arranged by size: 2626 1504 705 314 220 193 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2304 3339 3585 669 Size: 988 281 366 1035 246 3079 Fragments arranged by size: 3079 1035 988 366 281 246 HindIII A'AGCT_T Cuts at: 748 3012 748 Size: 2264 3731 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 5109 5293 1305 Size: 891 2913 184 2007 Fragments arranged by size: 2913 2007 891 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2271 3953 4312 4471 518 Size: 648 1105 1682 359 159 2042 Fragments arranged by size: 2042 1682 1105 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 3411 4088 5236 5481 1606 Size: 504 1301 677 1148 245 2120 Fragments arranged by size: 2120 1301 1148 677 504 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 3353 4898 1132 Size: 97 2124 1545 2229 Fragments arranged by size: 2229 2124 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5995 PciI A'CATG_T Cuts at: 4894 4894 Size: 5995 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5995 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5995 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PstI C_TGCA'G Cuts at: 830 2737 3347 830 Size: 1907 610 3478 Fragments arranged by size: 3478 1907 610 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2110 2110 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2158 2733 3343 4478 5159 5350 826 Size: 219 1113 575 610 1135 681 191 1471 Fragments arranged by size: 1471 1135 1113 681 610 575 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 3322 1872 Size: 1450 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2504 4099 5000 5262 820 Size: 197 926 561 1595 901 262 1553 Fragments arranged by size: 1595 1553 926 901 561 262 197 SnaBI TAC'GTA Cuts at: 493 2653 3786 493 Size: 2160 1133 2702 Fragments arranged by size: 2702 2160 1133 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 2320 3919 5 Size: 47 2268 1599 2081 Fragments arranged by size: 2268 2081 1599 47 StuI AGG'CCT Cuts at: 1517 2762 1517 Size: 1245 4750 StyI C'CwwG_G Cuts at: 513 1065 2004 2392 3434 513 Size: 552 939 388 1042 3074 Fragments arranged by size: 3074 1042 939 552 388 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2224 4022 4039 4192 4377 4822 325 Size: 1255 644 1798 17 153 185 445 1498 Fragments arranged by size: 1798 1498 1255 644 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4241 5953 96 Size: 274 80 33 51 1443 2264 1712 138 Fragments arranged by size: 2264 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 3320 1870 Size: 1450 4545 XmnI GAAnn'nnTTC Cuts at: 2566 2762 2930 4124 2566 Size: 196 168 1194 4437 Fragments arranged by size: 4437 1194 196 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI