(Circular) (Six-base) MAPSORT of: pFN21AB7386.seq Check: 5011 from: 1 to: 5959 pFN21AB7386 5959 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2086 2086 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2529 2640 4022 5406 5674 1632 Size: 897 111 1382 1384 268 1917 Fragments arranged by size: 1917 1384 1382 897 268 111 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 2305 2663 3038 3702 5274 2305 Size: 358 375 664 1572 2990 Fragments arranged by size: 2990 1572 664 375 358 ApaLI G'TGCA_C Cuts at: 3220 4016 5172 3220 Size: 796 1156 4007 Fragments arranged by size: 4007 1156 796 ApoI r'AATT_y Cuts at: 1493 2138 2498 2611 2798 3268 3494 3646 5755 Size: 645 360 113 187 470 226 152 2109 Cuts at: 5755 1493 Size: 1697 Fragments arranged by size: 2109 1697 645 470 360 226 187 152 113 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 2630 3289 3656 2630 Size: 659 367 4933 Fragments arranged by size: 4933 659 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3190 3280 4735 618 Size: 325 217 627 1403 90 1455 1842 Fragments arranged by size: 1842 1455 1403 627 325 217 90 BanII G_rGCy'C Cuts at: 1391 3278 1391 Size: 1887 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 2919 928 Size: 971 126 894 3968 Fragments arranged by size: 3968 971 894 126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 3224 4020 5176 1790 Size: 1434 796 1156 2573 Fragments arranged by size: 2573 1434 1156 796 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2415 4619 1239 Size: 1176 2204 2579 Fragments arranged by size: 2579 2204 1176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2305 2679 4084 4949 5247 2305 Size: 374 1405 865 298 3017 Fragments arranged by size: 3017 1405 865 374 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2944 2947 1186 Size: 1758 3 4198 Fragments arranged by size: 4198 1758 3 BseYI C'CCAG_C Cuts at: 5162 5658 5162 Size: 496 5463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2120 2688 1163 Size: 573 384 568 4434 Fragments arranged by size: 4434 573 568 384 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3224 4020 4105 5176 3224 Size: 796 85 1071 4007 Fragments arranged by size: 4007 1071 796 85 BsmI GAATG_Cn' Cuts at: 2110 2155 2872 3470 3563 2110 Size: 45 717 598 93 4506 Fragments arranged by size: 4506 717 598 93 45 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3224 3278 4020 4105 5176 1391 Size: 399 1434 54 742 85 1071 2174 Fragments arranged by size: 2174 1434 1071 742 399 85 54 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2086 2100 3314 844 Size: 1242 14 1214 3489 Fragments arranged by size: 3489 1242 1214 14 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2115 3237 4454 4628 65 Size: 1183 867 1122 1217 174 1396 Fragments arranged by size: 1396 1217 1183 1122 867 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 2998 96 Size: 2902 3057 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2053 2630 3289 3656 4041 4058 5510 5596 Size: 81 577 659 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 659 577 385 367 346 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 2682 513 Size: 552 1617 3790 Fragments arranged by size: 3790 1617 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3222 3475 4269 4289 3222 Size: 253 794 20 4892 Fragments arranged by size: 4892 794 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 2317 2503 2748 3263 2317 Size: 186 245 515 5013 Fragments arranged by size: 5013 515 245 186 DraIII CAC_nnn'GTG Cuts at: 2897 2897 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2808 3406 4295 Size: 54 1425 50 275 18 978 598 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 978 889 598 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2175 2520 2593 2965 3230 3235 3889 1353 Size: 822 345 73 372 265 5 654 3423 Fragments arranged by size: 3423 822 654 372 345 265 73 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2529 2640 4022 4619 5406 5674 Size: 393 783 114 111 1382 597 787 268 Cuts at: 5674 1239 Size: 1524 Fragments arranged by size: 1524 1382 787 783 597 393 268 114 111 EcoRI G'AATT_C Cuts at: 2138 2611 3268 3646 2138 Size: 473 657 378 4451 Fragments arranged by size: 4451 657 473 378 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 2464 2496 4600 Size: 32 135 32 782 32 78 32 2104 Cuts at: 4600 4632 4674 4706 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2104 782 135 78 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 2217 748 Size: 1469 4490 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2480 3157 5073 5257 1305 Size: 1175 677 1916 184 2007 Fragments arranged by size: 2007 1916 1175 677 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2810 10 Size: 54 1425 343 978 3159 Fragments arranged by size: 3159 1425 978 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 3917 4276 4435 518 Size: 648 909 1842 359 159 2042 Fragments arranged by size: 2042 1842 909 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2690 3375 4052 5200 5445 1606 Size: 1084 685 677 1148 245 2120 Fragments arranged by size: 2120 1148 1084 685 677 245 NcoI C'CATG_G Cuts at: 513 1065 2682 513 Size: 552 1617 3790 Fragments arranged by size: 3790 1617 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 2626 1108 Size: 1518 4441 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 2097 3311 830 Size: 1267 1214 3478 Fragments arranged by size: 3478 1267 1214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2690 2690 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 2175 2520 2175 Size: 345 5614 SbfI CC_TGCA'GG Cuts at: 2097 3311 2097 Size: 1214 4745 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2093 2709 3024 3150 3307 4442 5123 Size: 219 1048 616 315 126 157 1135 681 Cuts at: 5123 5314 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1048 681 616 315 219 191 157 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2284 2694 4063 4964 5226 820 Size: 197 926 341 410 1369 901 262 1553 Fragments arranged by size: 1553 1369 926 901 410 341 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 2358 3883 5 Size: 47 2306 1525 2081 Fragments arranged by size: 2306 2081 1525 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2682 3398 513 Size: 552 1617 716 3074 Fragments arranged by size: 3074 1617 716 552 SwaI ATTT'AAAT Cuts at: 2503 2503 Size: 5959 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3986 4003 4156 4341 4786 325 Size: 1255 2406 17 153 185 445 1498 Fragments arranged by size: 2406 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2998 4205 5917 Size: 274 80 33 51 1443 1021 1207 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1712 1443 1207 1021 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3201 3201 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 2514 4088 2514 Size: 1574 4385 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI