Restriction map for
pFN21AB7386
(Linear) (Six-base) MAPSORT of: KIBB7386.seq Check: 735 from: 1 to: 1254
KIBB7386.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 81 1254
Size: 81 1173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 635 1254
Size: 524 111 619
Fragments arranged by size:
619 524 111
AlwNI CAG_nnn'CTG
Cuts at: 0 300 658 1033 1254
Size: 300 358 375 221
Fragments arranged by size:
375 358 300 221
ApaLI G'TGCA_C
Cuts at: 0 1215 1254
Size: 1215 39
ApoI r'AATT_y
Cuts at: 0 133 493 606 793 1254
Size: 133 360 113 187 461
Fragments arranged by size:
461 360 187 133 113
BamHI G'GATC_C
Cuts at: 0 625 1254
Size: 625 629
BanI G'GyrC_C
Cuts at: 0 1185 1254
Size: 1185 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 914 1254
Size: 20 894 340
Fragments arranged by size:
894 340 20
Bme1580I G_kGCm'C
Cuts at: 0 1219 1254
Size: 1219 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1254
Size: 410 844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 674 1254
Size: 300 374 580
Fragments arranged by size:
580 374 300
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 939 942 1254
Size: 939 3 312
Fragments arranged by size:
939 312 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 683 1254
Size: 115 568 571
Fragments arranged by size:
571 568 115
BsiHKAI G_wGCw'C
Cuts at: 0 1219 1254
Size: 1219 35
BsmI GAATG_Cn'
Cuts at: 0 105 150 867 1254
Size: 105 45 717 387
Fragments arranged by size:
717 387 105 45
Bsp1286I G_dGCh'C
Cuts at: 0 1219 1254
Size: 1219 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 81 95 1254
Size: 81 14 1159
Fragments arranged by size:
1159 81 14
BsrDI GCAATG_nn'
Cuts at: 0 110 1232 1254
Size: 110 1122 22
Fragments arranged by size:
1122 110 22
BsrGI T'GTAC_A
Cuts at: 0 993 1254
Size: 993 261
BstYI r'GATC_y
Cuts at: 0 48 625 1254
Size: 48 577 629
Fragments arranged by size:
629 577 48
BtgI C'CryG_G
Cuts at: 0 677 1254
Size: 677 577
BtsI GCAGTG_nn'
Cuts at: 0 1217 1254
Size: 1217 37
DraI TTT'AAA
Cuts at: 0 312 498 743 1254
Size: 312 186 245 511
Fragments arranged by size:
511 312 245 186
DraIII CAC_nnn'GTG
Cuts at: 0 892 1254
Size: 892 362
EaeI y'GGCC_r
Cuts at: 0 803 1254
Size: 803 451
EarI CTCTTCn'nnn_
Cuts at: 0 170 515 588 960 1225 1230 1254
Size: 170 345 73 372 265 5 24
Fragments arranged by size:
372 345 265 170 73 24 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 524 635 1254
Size: 410 114 111 619
Fragments arranged by size:
619 410 114 111
EcoRI G'AATT_C
Cuts at: 0 133 606 1254
Size: 133 473 648
Fragments arranged by size:
648 473 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 459 491 1254
Size: 349 32 78 32 763
Fragments arranged by size:
763 349 78 32 32
HindIII A'AGCT_T
Cuts at: 0 212 1254
Size: 212 1042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1152 1254
Size: 475 677 102
Fragments arranged by size:
677 475 102
MscI TGG'CCA
Cuts at: 0 805 1254
Size: 805 449
MslI CAynn'nnrTG
Cuts at: 0 70 1254
Size: 70 1184
MspA1I CmG'CkG
Cuts at: 0 685 1254
Size: 685 569
NcoI C'CATG_G
Cuts at: 0 677 1254
Size: 677 577
PflMI CCAn_nnn'nTGG
Cuts at: 0 621 1254
Size: 621 633
PstI C_TGCA'G
Cuts at: 0 92 1254
Size: 92 1162
PvuII CAG'CTG
Cuts at: 0 685 1254
Size: 685 569
SapI GCTCTTCn'nnn_
Cuts at: 0 170 515 1254
Size: 170 345 739
Fragments arranged by size:
739 345 170
SbfI CC_TGCA'GG
Cuts at: 0 92 1254
Size: 92 1162
SfcI C'TryA_G
Cuts at: 0 88 704 1019 1145 1254
Size: 88 616 315 126 109
Fragments arranged by size:
616 315 126 109 88
SmlI C'TyrA_G
Cuts at: 0 279 689 1254
Size: 279 410 565
Fragments arranged by size:
565 410 279
SspI AAT'ATT
Cuts at: 0 353 1254
Size: 353 901
StyI C'CwwG_G
Cuts at: 0 677 1254
Size: 677 577
SwaI ATTT'AAAT
Cuts at: 0 498 1254
Size: 498 756
TatI w'GTAC_w
Cuts at: 0 993 1254
Size: 993 261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1196 1254
Size: 1196 58
XmnI GAAnn'nnTTC
Cuts at: 0 509 1254
Size: 509 745
Enzymes that do cut:
AarI AcuI AlwNI ApaLI ApoI BamHI BanI BbsI
Bme1580I BpmI BpuEI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrGI BstYI BtgI BtsI DraI DraIII
EaeI EarI Eco57MI EcoRI Hin4I HindIII MmeI MscI
MslI MspA1I NcoI PflMI PstI PvuII SapI SbfI
SfcI SmlI SspI StyI SwaI TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI TaqII TstI Tth111I
XbaI XhoI XmaI ZraI