Restriction map for
pFN21AB7384
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsgI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I FauI
FbaI FseI FspI HaeII Hin1I
KasI KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 718 CGCG CG^CG
AccIII 89 TCCGGA T^CCGGA
AflIII 203 ACACGT A^C[GA][CT]GT
Asp700I 627 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BglII 1319 AGATCT A^GATCT
BpmI 1211 CTCCAG CTGGAG(16/14)
BsaAI 204 CACGTA [CT]AC^GT[GA]
BsaWI 89 TCCGGA [AT]^CCGG[AT]
BseAI 89 TCCGGA T^CCGGA
BsmAI 1047 GTCTC GTCTC(1/5)
BspEI 89 TCCGGA T^CCGGA
BstUI 718 CGCG CG^CG
BstXI 636 CCATTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 410 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 111 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 275 CCATGG C^C[GA][CT]GG
EcoNI 1108 CCTAGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 410 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 467 GAATTC G^AATTC
EcoRV 729 GATATC GAT^ATC
EcoT14I 275 CCATGG C^C[AT][AT]GG
EcoT22I 580 ATGCAT ATGCA^T
HindIII 657 AAGCTT A^AGCTT
HpaI 961 GTTAAC GTT^AAC
MroI 89 TCCGGA T^CCGGA
MvnI 718 CGCG CG^CG
NcoI 275 CCATGG C^CATGG
NotI 55 GCGGCCGC GC^GGCCGC
NsiI 580 ATGCAT ATGCA^T
PflMI 967 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 410 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 811 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 302 CTGCAG CTGCA^G
SacI 613 GAGCTC GAGCT^C
SapI 232 GAAGAGC GCTCTTC(1/4)
StyI 275 CCATGG C^C[AT][AT]GG
Van91I 967 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 627 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 646 GGTACC G^GTACC
1162 GGTACC
AcsI 467 GAATTC [GA]^AATT[CT]
1382 AAATTC
AlwI 291 GATCC GGATC(4/5)
988 GATCC
ApoI 467 GAATTC [GA]^AATT[CT]
1382 AAATTC
Asp718I 646 GGTACC G^GTACC
1162 GGTACC
AvaII 411 GGTCC G^G[AT]CC
442 GGACC
BalI 278 TGGCCA TGG^CCA
1284 TGGCCA
BanII 235 GAGCCC G[GA]GC[CT]^C
613 GAGCTC
BbsI 883 GAAGAC GAAGAC(2/6)
1005 GAAGAC
BpuAI 883 GAAGAC GAAGAC(2/6)
1005 GAAGAC
BsiEI 56 CGGCCG CG[GA][CT]^CG
159 CGGCCG
BstF5I 1075 CATCC GGATG(2/0)
539 GGATG
BstNI 165 CCAGG CC^[AT]GG
354 CCTGG
BstYI 290 AGATCC [GA]^GATC[CT]
1319 AGATCT
CfoI 719 GCGC GCG^C
1014 GCGC
EagI 56 CGGCCG C^GGCCG
159 CGGCCG
EclXI 56 CGGCCG C^GGCCG
159 CGGCCG
Eco52I 56 CGGCCG C^GGCCG
159 CGGCCG
EcoRII 165 CCAGG ^CC[AT]GG
354 CCTGG
FokI 1075 CATCC GGATG(9/13)
539 GGATG
HgaI 101 GCGTC GACGC(5/10)
119 GCGTC
HhaI 719 GCGC GCG^C
1014 GCGC
HinP1I 719 GCGC G^CGC
1014 GCGC
HphI 229 GGTGA GGTGA(8/7)
1207 TCACC
KpnI 646 GGTACC GGTAC^C
1162 GGTACC
MaeII 143 ACGT A^CGT
205 ACGT
MflI 290 AGATCC [GA]^GATC[CT]
1319 AGATCT
MluNI 278 TGGCCA TGG^CCA
1284 TGGCCA
MscI 278 TGGCCA TGG^CCA
1284 TGGCCA
MslI 637 CATTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1231 CACGACTGTG
MspA1I 299 CAGCTG C[AC]G^C[GT]G
1361 CAGCTG
MvaI 165 CCAGG CC^[AT]GG
354 CCTGG
PvuII 299 CAGCTG CAG^CTG
1361 CAGCTG
TaqI 333 TCGA T^CGA
741 TCGA
Tsp45I 250 GTCAC ^GT[GC]AC
532 GTGAC
TspRI 185 CACTG CA[GC]TG[ACGT][ACGT]^
252 CACTG
TthHB8I 333 TCGA T^CGA
741 TCGA
XhoII 290 AGATCC [GA]^GATC[CT]
1319 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AspHI BanI
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI Cac8I Cfr13I
DdeI DpnII EaeI EarI Fnu4HI
HaeIII HapII HincII HindII HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp509I