(Circular) (Six-base) MAPSORT of: pFN21AB7384.seq Check: 8015 from: 1 to: 6097 pFN21AB7384 6097 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 2651 3167 3418 2651 Size: 516 251 5330 Fragments arranged by size: 5330 516 251 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4160 5544 5812 1632 Size: 2528 1384 268 1917 Fragments arranged by size: 2528 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 2208 4996 5973 1203 Size: 1005 2788 977 1327 Fragments arranged by size: 2788 1327 1005 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 3240 1166 Size: 2074 4023 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 3840 5412 3840 Size: 1572 4525 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2472 3387 3406 3632 3784 5893 1493 Size: 979 915 19 226 152 2109 1697 Fragments arranged by size: 2109 1697 979 915 226 152 19 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 3422 1870 Size: 73 1479 4545 Fragments arranged by size: 4545 1479 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2635 2643 2668 2676 2635 Size: 8 25 8 6056 Fragments arranged by size: 6056 25 8 8 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2131 2651 3167 3418 4873 Size: 325 217 627 344 520 516 251 1455 Cuts at: 4873 618 Size: 1842 Fragments arranged by size: 1842 1455 627 520 516 344 325 251 217 BanII G_rGCy'C Cuts at: 1391 2244 2622 3416 1391 Size: 853 378 794 4072 Fragments arranged by size: 4072 853 794 378 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2895 3017 928 Size: 971 996 122 4008 Fragments arranged by size: 4008 996 971 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BfrBI ATG'CAT Cuts at: 2587 2587 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4708 136 Size: 107 122 71 631 605 337 2699 1525 Fragments arranged by size: 2699 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3324 6092 3324 Size: 2768 3329 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 2136 4158 5314 1790 Size: 346 2022 1156 2573 Fragments arranged by size: 2573 2022 1156 346 BmgBI CAC'GTC Cuts at: 1318 2149 1318 Size: 831 5266 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3828 4786 442 Size: 416 53 83 399 24 2411 958 1753 Fragments arranged by size: 2411 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2315 2317 2335 2315 Size: 2 18 6077 Fragments arranged by size: 6077 18 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3201 4757 1239 Size: 1962 1556 2579 Fragments arranged by size: 2579 1962 1556 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 882 Size: 617 3261 2219 Fragments arranged by size: 3261 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2211 3888 493 Size: 711 1007 1677 2702 Fragments arranged by size: 2702 1677 1007 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2094 4530 4928 5202 5349 5760 2094 Size: 2436 398 274 147 411 2431 Fragments arranged by size: 2436 2431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5872 Fragments arranged by size: 5872 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2689 3033 3172 1186 Size: 1503 344 139 4111 Fragments arranged by size: 4111 1503 344 139 BseYI C'CCAG_C Cuts at: 2135 5300 5796 2135 Size: 3165 496 2436 Fragments arranged by size: 3165 2436 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2064 2167 3547 4308 5336 1540 Size: 275 186 63 103 1380 761 1028 2301 Fragments arranged by size: 2301 1380 1028 761 275 186 103 63 BsiHKAI G_wGCw'C Cuts at: 2148 2316 2569 2622 4158 4243 5314 2148 Size: 168 253 53 1536 85 1071 2931 Fragments arranged by size: 2931 1536 1071 253 168 85 53 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2148 2244 2316 2569 2622 3416 Size: 399 346 12 96 72 253 53 794 Cuts at: 3416 4158 4243 5314 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 794 742 399 346 253 96 85 72 53 12 BspEI T'CCGG_A Cuts at: 2094 5760 2094 Size: 3666 2431 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3984 1394 Size: 429 2161 3507 Fragments arranged by size: 3507 2161 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4741 4928 1700 Size: 110 2931 187 2869 Fragments arranged by size: 2931 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstXI CCAn_nnnn'nTGG Cuts at: 2648 2648 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2295 3324 3427 3794 4179 4196 5648 5734 Size: 323 1029 103 367 385 17 1452 86 Cuts at: 5734 5746 6092 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1029 385 367 346 323 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2280 513 Size: 552 1215 4330 Fragments arranged by size: 4330 1215 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2030 487 Size: 683 56 804 4554 Fragments arranged by size: 4554 804 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DraIII CAC_nnn'GTG Cuts at: 2121 2121 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2164 2283 Size: 54 1425 50 275 18 231 103 119 Cuts at: 2283 3289 3544 4433 6062 8 Size: 1006 255 889 1629 43 Fragments arranged by size: 1629 1425 1006 889 275 255 231 119 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2061 2164 3544 1537 Size: 275 249 103 1380 4090 Fragments arranged by size: 4090 1380 275 249 103 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 2622 3190 3305 4027 1353 Size: 879 390 568 115 722 3423 Fragments arranged by size: 3423 879 722 568 390 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2620 2620 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3201 4160 4757 5544 5812 1239 Size: 393 1569 959 597 787 268 1524 Fragments arranged by size: 1569 1524 959 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3117 3117 Size: 6097 EcoO109I rG'GnC_Cy Cuts at: 2416 2416 Size: 6097 EcoRI G'AATT_C Cuts at: 2472 3406 3784 2472 Size: 934 378 4785 Fragments arranged by size: 4785 934 378 EcoRV GAT'ATC Cuts at: 2736 2736 Size: 6097 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373 Size: 32 135 32 3166 32 42 32 2626 Fragments arranged by size: 3166 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2110 2560 2902 2968 3441 3687 Size: 988 281 172 450 342 66 473 246 Cuts at: 3687 669 Size: 3079 Fragments arranged by size: 3079 988 473 450 342 281 246 172 66 HindIII A'AGCT_T Cuts at: 748 2662 748 Size: 1914 4183 HpaI GTT'AAC Cuts at: 2968 3687 2968 Size: 719 5378 KpnI G_GTAC'C Cuts at: 2655 3171 3422 2655 Size: 516 251 5330 Fragments arranged by size: 5330 516 251 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2425 2849 5211 5395 1305 Size: 1120 424 2362 184 2007 Fragments arranged by size: 2362 2007 1120 424 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2285 3291 10 Size: 54 1425 343 453 1006 2816 Fragments arranged by size: 2816 1425 1006 453 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2646 3240 4055 4414 4573 518 Size: 648 1480 594 815 359 159 2042 Fragments arranged by size: 2042 1480 815 648 594 359 159 MspA1I CmG'CkG Cuts at: 1606 2306 3368 3513 4190 5338 5583 1606 Size: 700 1062 145 677 1148 245 2120 Fragments arranged by size: 2120 1148 1062 700 677 245 145 NcoI C'CATG_G Cuts at: 513 1065 2280 513 Size: 552 1215 4330 Fragments arranged by size: 4330 1215 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 2061 3544 2061 Size: 1483 4614 NsiI A_TGCA'T Cuts at: 2589 2589 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 3455 5000 1132 Size: 97 2226 1545 2229 Fragments arranged by size: 2229 2226 1545 97 PasI CC'CwG_GG Cuts at: 1348 2359 1348 Size: 1011 5086 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 2978 1108 Size: 1870 4227 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2416 2416 Size: 6097 PshAI GACnn'nnGTC Cuts at: 1613 2820 1613 Size: 1207 4890 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PstI C_TGCA'G Cuts at: 830 2311 3449 830 Size: 1481 1138 3478 Fragments arranged by size: 3478 1481 1138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 2306 3368 2306 Size: 1062 5035 SacI G_AGCT'C Cuts at: 2622 2622 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SanDI GG'GwC_CC Cuts at: 2416 2416 Size: 6097 SapI GCTCTTCn'nnn_ Cuts at: 2232 2232 Size: 6097 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 2307 2781 2877 3445 4580 5261 5452 Size: 219 1262 474 96 568 1135 681 191 Cuts at: 5452 826 Size: 1471 Fragments arranged by size: 1471 1262 1135 681 568 474 219 191 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4201 5102 5364 820 Size: 197 926 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2280 3536 513 Size: 552 1215 1256 3074 Fragments arranged by size: 3074 1256 1215 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2577 4124 4141 4294 4479 4924 325 Size: 1255 997 1547 17 153 185 445 1498 Fragments arranged by size: 1547 1498 1255 997 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3116 4343 6055 Size: 274 80 33 51 1443 1139 1227 1712 Cuts at: 6055 96 Size: 138 Fragments arranged by size: 1712 1443 1227 1139 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 2636 4226 2636 Size: 1590 4507 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI