Restriction map for
pFN21AB7384
(Linear) (Six-base) MAPSORT of: KIBB7384.seq Check: 8730 from: 1 to: 1392
KIBB7384.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
Acc65I G'GTAC_C
Cuts at: 0 646 1162 1392
Size: 646 516 230
Fragments arranged by size:
646 516 230
AflIII A'CryG_T
Cuts at: 0 203 1392
Size: 203 1189
AleI CACnn'nnGTG
Cuts at: 0 1235 1392
Size: 1235 157
ApoI r'AATT_y
Cuts at: 0 467 1382 1392
Size: 467 915 10
Fragments arranged by size:
915 467 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 630 638 663 671 1392
Size: 630 8 25 8 721
Fragments arranged by size:
721 630 25 8 8
BanI G'GyrC_C
Cuts at: 0 126 646 1162 1392
Size: 126 520 516 230
Fragments arranged by size:
520 516 230 126
BanII G_rGCy'C
Cuts at: 0 239 617 1392
Size: 239 378 775
Fragments arranged by size:
775 378 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 890 1012 1392
Size: 890 122 380
Fragments arranged by size:
890 380 122
BfrBI ATG'CAT
Cuts at: 0 582 1392
Size: 582 810
BglII A'GATC_T
Cuts at: 0 1319 1392
Size: 1319 73
Bme1580I G_kGCm'C
Cuts at: 0 131 1392
Size: 131 1261
BmgBI CAC'GTC
Cuts at: 0 144 1392
Size: 144 1248
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 310 312 330 1392
Size: 310 2 18 1062
Fragments arranged by size:
1062 310 18 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1392
Size: 1196 196
BsaAI yAC'GTr
Cuts at: 0 206 1392
Size: 206 1186
BsaWI w'CCGG_w
Cuts at: 0 89 1392
Size: 89 1303
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 684 1028 1167 1392
Size: 684 344 139 225
Fragments arranged by size:
684 344 225 139
BseYI C'CCAG_C
Cuts at: 0 130 1392
Size: 130 1262
BsiEI CG_ry'CG
Cuts at: 0 59 162 1392
Size: 59 103 1230
Fragments arranged by size:
1230 103 59
BsiHKAI G_wGCw'C
Cuts at: 0 143 311 564 617 1392
Size: 143 168 253 53 775
Fragments arranged by size:
775 253 168 143 53
Bsp1286I G_dGCh'C
Cuts at: 0 131 143 239 311 564 617 1392
Size: 131 12 96 72 253 53 775
Fragments arranged by size:
775 253 131 96 72 53 12
BspEI T'CCGG_A
Cuts at: 0 89 1392
Size: 89 1303
BstXI CCAn_nnnn'nTGG
Cuts at: 0 643 1392
Size: 643 749
BstYI r'GATC_y
Cuts at: 0 290 1319 1392
Size: 290 1029 73
Fragments arranged by size:
1029 290 73
BtgI C'CryG_G
Cuts at: 0 275 1392
Size: 275 1117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 25 1392
Size: 25 1367
DraIII CAC_nnn'GTG
Cuts at: 0 116 1392
Size: 116 1276
EaeI y'GGCC_r
Cuts at: 0 56 159 278 1284 1392
Size: 56 103 119 1006 108
Fragments arranged by size:
1006 119 108 103 56
EagI C'GGCC_G
Cuts at: 0 56 159 1392
Size: 56 103 1233
Fragments arranged by size:
1233 103 56
EarI CTCTTCn'nnn_
Cuts at: 0 227 617 1185 1300 1392
Size: 227 390 568 115 92
Fragments arranged by size:
568 390 227 115 92
EcoICRI GAG'CTC
Cuts at: 0 615 1392
Size: 615 777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1392
Size: 1196 196
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1112 1392
Size: 1112 280
EcoO109I rG'GnC_Cy
Cuts at: 0 411 1392
Size: 411 981
EcoRI G'AATT_C
Cuts at: 0 467 1392
Size: 467 925
EcoRV GAT'ATC
Cuts at: 0 731 1392
Size: 731 661
HincII GTy'rAC
Cuts at: 0 105 555 897 963 1392
Size: 105 450 342 66 429
Fragments arranged by size:
450 429 342 105 66
HindIII A'AGCT_T
Cuts at: 0 657 1392
Size: 657 735
HpaI GTT'AAC
Cuts at: 0 963 1392
Size: 963 429
KpnI G_GTAC'C
Cuts at: 0 650 1166 1392
Size: 650 516 226
Fragments arranged by size:
650 516 226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 844 1392
Size: 420 424 548
Fragments arranged by size:
548 424 420
MscI TGG'CCA
Cuts at: 0 280 1286 1392
Size: 280 1006 106
Fragments arranged by size:
1006 280 106
MslI CAynn'nnrTG
Cuts at: 0 641 1235 1392
Size: 641 594 157
Fragments arranged by size:
641 594 157
MspA1I CmG'CkG
Cuts at: 0 301 1363 1392
Size: 301 1062 29
Fragments arranged by size:
1062 301 29
NcoI C'CATG_G
Cuts at: 0 275 1392
Size: 275 1117
NotI GC'GGCC_GC
Cuts at: 0 56 1392
Size: 56 1336
NsiI A_TGCA'T
Cuts at: 0 584 1392
Size: 584 808
PasI CC'CwG_GG
Cuts at: 0 354 1392
Size: 354 1038
PflMI CCAn_nnn'nTGG
Cuts at: 0 973 1392
Size: 973 419
PpuMI rG'GwC_Cy
Cuts at: 0 411 1392
Size: 411 981
PshAI GACnn'nnGTC
Cuts at: 0 815 1392
Size: 815 577
PstI C_TGCA'G
Cuts at: 0 306 1392
Size: 306 1086
PvuII CAG'CTG
Cuts at: 0 301 1363 1392
Size: 301 1062 29
Fragments arranged by size:
1062 301 29
SacI G_AGCT'C
Cuts at: 0 617 1392
Size: 617 775
SanDI GG'GwC_CC
Cuts at: 0 411 1392
Size: 411 981
SapI GCTCTTCn'nnn_
Cuts at: 0 227 1392
Size: 227 1165
SfcI C'TryA_G
Cuts at: 0 302 776 872 1392
Size: 302 474 96 520
Fragments arranged by size:
520 474 302 96
StyI C'CwwG_G
Cuts at: 0 275 1392
Size: 275 1117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 572 1392
Size: 572 820
TatI w'GTAC_w
Cuts at: 0 1111 1392
Size: 1111 281
XmnI GAAnn'nnTTC
Cuts at: 0 631 1392
Size: 631 761
Enzymes that do cut:
Acc65I AflIII AleI ApoI BaeI BanI BanII BbsI
BfrBI BglII Bme1580I BmgBI BplI BpmI BsaAI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BstXI BstYI
BtgI BtgZI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV HincII HindIII HpaI KpnI
MmeI MscI MslI MspA1I NcoI NotI NsiI PasI
PflMI PpuMI PshAI PstI PvuII SacI SanDI SapI
SfcI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI BciVI
BclI BglI BlpI BmrI BmtI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BsgI BsiWI BsmI BsmBI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI
DrdI EciI FalI FseI FspI FspAI HaeII Hin4I
KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NruI NspI PacI PciI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI