(Circular) (Six-base) MAPSORT of: pFN21AB7381.seq Check: 2851 from: 1 to: 6091 pFN21AB7381 6091 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2677 2693 2677 Size: 16 6075 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2416 3434 1937 Size: 479 1018 4594 Fragments arranged by size: 4594 1018 479 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2576 2914 3044 4154 5538 5806 1632 Size: 944 338 130 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1110 944 338 268 130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2232 4990 5967 1203 Size: 1029 2758 977 1327 Fragments arranged by size: 2758 1327 1029 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 2344 3834 5406 2344 Size: 1490 1572 3029 Fragments arranged by size: 3029 1572 1490 ApaI G_GGCC'C Cuts at: 2797 2946 3113 3262 3371 2797 Size: 149 167 149 109 5517 Fragments arranged by size: 5517 167 149 149 109 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2763 3223 3400 3626 3778 5887 1493 Size: 1270 460 177 226 152 2109 1697 Fragments arranged by size: 2109 1697 1270 460 226 177 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2125 2314 2936 3254 3416 1870 Size: 73 182 189 622 318 162 4545 Fragments arranged by size: 4545 622 318 189 182 162 73 BamHI G'GATC_C Cuts at: 2974 3421 3788 2974 Size: 447 367 5277 Fragments arranged by size: 5277 447 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2365 2397 2699 3412 4867 Size: 325 217 627 578 32 302 713 1455 Cuts at: 4867 618 Size: 1842 Fragments arranged by size: 1842 1455 713 627 578 325 302 217 32 BanII G_rGCy'C Cuts at: 1391 2126 2484 2657 2797 2875 2946 3032 3113 Size: 735 358 173 140 78 71 86 81 Cuts at: 3113 3262 3371 3410 1391 Size: 149 109 39 4072 Fragments arranged by size: 4072 735 358 173 149 140 109 86 81 78 71 39 BbeI G_GCGC'C Cuts at: 2401 2401 Size: 6091 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2825 928 Size: 971 926 4194 Fragments arranged by size: 4194 971 926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2200 2234 2305 2339 4282 Size: 34 50 34 179 34 71 34 1943 Cuts at: 4282 4316 1903 Size: 34 3678 Fragments arranged by size: 3678 1943 179 71 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2499 6086 2499 Size: 3587 2504 BlpI GC'TnA_GC Cuts at: 3508 3508 Size: 6091 Bme1580I G_kGCm'C Cuts at: 1790 2370 2704 2797 2946 3113 3262 3371 4152 Size: 580 334 93 149 167 149 109 781 Cuts at: 4152 5308 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 781 580 334 167 149 149 109 93 BmgBI CAC'GTC Cuts at: 1318 2299 1318 Size: 981 5110 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 2432 3108 Size: 416 53 83 399 24 762 253 676 Cuts at: 3108 3822 4780 442 Size: 714 958 1753 Fragments arranged by size: 1753 958 762 714 676 416 399 253 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2140 2366 2841 4751 1239 Size: 901 226 475 1910 2579 Fragments arranged by size: 2579 1910 901 475 226 Bpu10I CC'TnA_GC Cuts at: 2279 2483 2773 3201 2279 Size: 204 290 428 5169 Fragments arranged by size: 5169 428 290 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3054 4216 5081 5379 3054 Size: 1162 865 298 3766 Fragments arranged by size: 3766 1162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4754 882 Size: 617 3255 2219 Fragments arranged by size: 3255 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2398 3898 4280 4915 Size: 53 83 186 936 865 1500 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1500 1451 936 865 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2735 2765 1178 Size: 30 165 30 1332 30 4504 Fragments arranged by size: 4504 1332 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6091 BseYI C'CCAG_C Cuts at: 2045 3106 3298 5294 5790 2045 Size: 1061 192 1996 496 2346 Fragments arranged by size: 2346 1996 1061 496 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2453 2471 2921 1163 Size: 573 717 18 450 4333 Fragments arranged by size: 4333 717 573 450 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2332 2392 3541 4302 5330 1540 Size: 275 186 331 60 1149 761 1028 2301 Fragments arranged by size: 2301 1149 1028 761 331 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2462 2484 2657 4152 4237 5308 2462 Size: 22 173 1495 85 1071 3245 Fragments arranged by size: 3245 1495 1071 173 85 22 BsmI GAATG_Cn' Cuts at: 2956 3602 3695 2956 Size: 646 93 5352 Fragments arranged by size: 5352 646 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2370 2462 2484 2657 2704 2797 Size: 399 336 244 92 22 173 47 93 Cuts at: 2797 2875 2946 3032 3113 3262 3371 3410 4152 Size: 78 71 86 81 149 109 39 742 Cuts at: 4152 4237 5308 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 336 244 173 149 109 93 92 86 85 81 78 71 47 39 22 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2677 2693 3094 3446 844 Size: 1833 16 401 352 3489 Fragments arranged by size: 3489 1833 401 352 16 BsrBI CCG'CTC Cuts at: 1394 1823 2130 3190 3978 1394 Size: 429 307 1060 788 3507 Fragments arranged by size: 3507 1060 788 429 307 BsrDI GCAATG_nn' Cuts at: 65 1248 2614 4586 4760 65 Size: 1183 1366 1972 174 1396 Fragments arranged by size: 1972 1396 1366 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 2440 794 Size: 1646 4445 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstYI r'GATC_y Cuts at: 1972 2499 2974 3421 3788 4173 4190 5642 5728 Size: 527 475 447 367 385 17 1452 86 Cuts at: 5728 5740 6086 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 527 475 447 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2834 2834 Size: 6091 BtgI C'CryG_G Cuts at: 513 1065 2031 2536 513 Size: 552 966 505 4068 Fragments arranged by size: 4068 966 552 505 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 2882 3607 4401 4421 2882 Size: 725 794 20 4552 Fragments arranged by size: 4552 794 725 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 2389 3538 Size: 54 1425 50 275 18 499 60 1149 Cuts at: 3538 4427 6056 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1149 889 499 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2329 2389 3538 1537 Size: 275 517 60 1149 4090 Fragments arranged by size: 4090 1149 517 275 60 EarI CTCTTCn'nnn_ Cuts at: 1353 4021 1353 Size: 2668 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2482 2655 2482 Size: 173 5918 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2366 2576 2841 2914 3044 4154 Size: 393 508 226 210 265 73 130 1110 Cuts at: 4154 4751 5538 5806 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1110 787 597 508 393 268 265 226 210 130 73 EcoNI CCTnn'n_nnAGG Cuts at: 2708 2967 2708 Size: 259 5832 EcoO109I rG'GnC_Cy Cuts at: 2042 2092 2407 2713 2793 2794 2942 2999 3092 Size: 50 315 306 80 1 148 57 93 Cuts at: 3092 3110 3244 3258 3259 2042 Size: 18 134 14 1 4874 Fragments arranged by size: 4874 315 306 148 134 93 80 57 50 18 14 1 1 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2018 2084 2401 5238 1678 Size: 340 66 317 2837 2531 Fragments arranged by size: 2837 2531 340 317 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2524 2556 2560 2592 2812 Size: 32 135 32 952 32 4 32 220 Cuts at: 2812 2844 4732 4764 4806 4838 1373 Size: 32 1888 32 42 32 2626 Fragments arranged by size: 2626 1888 952 220 135 42 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 2417 3435 3681 669 Size: 988 281 479 1018 246 3079 Fragments arranged by size: 3079 1018 988 479 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 2397 2397 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4269 Fragments arranged by size: 4269 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4049 4408 4567 518 Size: 648 2883 359 159 2042 Fragments arranged by size: 2883 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2021 2118 2394 3507 4184 5332 5577 1606 Size: 415 97 276 1113 677 1148 245 2120 Fragments arranged by size: 2120 1148 1113 677 415 276 245 97 NarI GG'CG_CC Cuts at: 2398 2398 Size: 6091 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5539 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 2329 3538 2329 Size: 1209 4882 NspI r_CATG'y Cuts at: 1132 1229 2236 3449 4994 1132 Size: 97 1007 1213 1545 2229 Fragments arranged by size: 2229 1545 1213 1007 97 PasI CC'CwG_GG Cuts at: 1348 2979 3236 3304 1348 Size: 1631 257 68 4135 Fragments arranged by size: 4135 1631 257 68 PciI A'CATG_T Cuts at: 2232 4990 2232 Size: 2758 3333 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PfoI T'CCnGG_A Cuts at: 2910 2910 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2407 2713 3244 2092 Size: 315 306 531 4939 Fragments arranged by size: 4939 531 315 306 PshAI GACnn'nnGTC Cuts at: 1613 2412 1613 Size: 799 5292 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PspOMI G'GGCC_C Cuts at: 2793 2942 3109 3258 3367 2793 Size: 149 167 149 109 5517 Fragments arranged by size: 5517 167 149 149 109 PstI C_TGCA'G Cuts at: 830 2712 3091 3443 830 Size: 1882 379 352 3478 Fragments arranged by size: 3478 1882 379 352 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2021 2021 Size: 6091 SacI G_AGCT'C Cuts at: 2484 2657 2484 Size: 173 5918 SalI G'TCGA_C Cuts at: 1936 2415 3433 1936 Size: 479 1018 4594 Fragments arranged by size: 4594 1018 479 SanDI GG'GwC_CC Cuts at: 2092 2092 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3091 3443 3091 Size: 352 5739 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SexAI A'CCwGG_T Cuts at: 2695 2695 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 2708 3087 3439 4574 5255 5446 826 Size: 219 1663 379 352 1135 681 191 1471 Fragments arranged by size: 1663 1471 1135 681 379 352 219 191 SfoI GGC'GCC Cuts at: 2399 2399 Size: 6091 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3069 4195 5096 5358 820 Size: 197 926 1126 1126 901 262 1553 Fragments arranged by size: 1553 1126 1126 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2169 3121 3177 3288 3530 513 Size: 552 1104 952 56 111 242 3074 Fragments arranged by size: 3074 1104 952 552 242 111 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4118 4135 4288 4473 4918 325 Size: 1255 2538 17 153 185 445 1498 Fragments arranged by size: 2538 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4337 6049 96 Size: 274 80 33 51 1443 2360 1712 138 Fragments arranged by size: 2360 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 2297 1316 Size: 981 5110 XbaI T'CTAG_A Cuts at: 3097 3427 3097 Size: 330 5761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2579 2579 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI