Restriction map for
pFN21AB7380
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BciVI BcnI BfaI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AflII 571 CTTAAG C^TTAAG
AocI 166 CCTGAGG CC^T[ACGT]AGG
BamHI 979 GGATCC G^GATCC
BbsI 260 GAAGAC GAAGAC(2/6)
BcgI 381 CGAGCAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 571 CTTAAG C^TTAAG
BglII 116 AGATCT A^GATCT
BpuAI 260 GAAGAC GAAGAC(2/6)
BsaI 902 GGTCTC GGTCTC(1/5)
BsaWI 45 TCCGGT [AT]^CCGG[AT]
BseRI 203 CTCCTC GAGGAG(10/8)
BsmFI 351 GGGAC GGGAC(10/14)
BspHI 183 TCATGA T^CATGA
BsrI 636 CCAGT ACTGG(1/-1)
BstXI 234 CCAGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 166 CCTGAGG CC^T[ACGT]AGG
DraII 351 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 166 CCTGAGG CC^T[ACGT]AGG
EcoO109I 351 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 620 GATATC GAT^ATC
HapII 46 CCGG C^CGG
HinfI 370 GATTC G^A[ACGT]TC
HpaII 46 CCGG C^CGG
MaeII 585 ACGT A^CGT
MspA1I 671 CAGCTG C[AC]G^C[GT]G
MspI 46 CCGG C^CGG
PflMI 636 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 351 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 671 CAGCTG CAG^CTG
RcaI 183 TCATGA T^CATGA
SapI 406 GAAGAGC GCTCTTC(1/4)
TfiI 370 GATTC G^A[AT]TC
Van91I 636 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 358 GAATTT [GA]^AATT[CT]
539 AAATTC
AfaI 472 GTAC GT^AC
928 GTAC
AlwI 980 GATCC GGATC(4/5)
979 GGATC
ApoI 358 GAATTT [GA]^AATT[CT]
539 AAATTC
AvaII 51 GGTCC G^G[AT]CC
352 GGACC
BbvCI 990 CCTCAGC CCTCAGC(-5/-2)
136 GCTGAGG
BclI 693 TGATCA T^GATCA
779 TGATCA
BglI 221 GCCTTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
230 GCCCCCAGGGC
BsmAI 26 GAGAC GTCTC(1/5)
903 GTCTC
BstF5I 69 CATCC GGATG(2/0)
163 CATCC
BstYI 116 AGATCT [GA]^GATC[CT]
979 GGATCC
Cac8I 384 GCAAGC GC[ACGT]^[ACGT]GC
411 GCTTGC
EarI 406 GAAGAG CTCTTC(1/4)
628 GAAGAG
FbaI 693 TGATCA T^GATCA
779 TGATCA
FokI 69 CATCC GGATG(9/13)
163 CATCC
Ksp632I 406 GAAGAG CTCTTC(1/4)
628 GAAGAG
MflI 116 AGATCT [GA]^GATC[CT]
979 GGATCC
NlaIII 184 CATG CATG^
789 CATG
RsaI 472 GTAC GT^AC
928 GTAC
SfaNI 667 GATGC GCATC(5/9)
162 GCATC
SmlI 571 CTTAAG C^T[CT][GA]AG
973 CTCAAG
TaqI 108 TCGA T^CGA
960 TCGA
TspRI 931 CAGTG CA[GC]TG[ACGT][ACGT]^
950 CACTG
TthHB8I 108 TCGA T^CGA
960 TCGA
XhoII 116 AGATCT [GA]^GATC[CT]
979 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI Asp700I BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII ItaI MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI Tru9I
TseI Tsp509I XmnI