(Circular) (Six-base) MAPSORT of: pFN21AB7380.seq Check: 8177 from: 1 to: 5713 pFN21AB7380 5713 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3056 1937 Size: 1119 4594 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3776 5160 5428 1632 Size: 2144 1384 268 1917 Fragments arranged by size: 2144 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2576 820 Size: 197 1559 3957 Fragments arranged by size: 3957 1559 197 AflIII A'CryG_T Cuts at: 1203 4612 5589 1203 Size: 3409 977 1327 Fragments arranged by size: 3409 1327 977 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5713 AlwNI CAG_nnn'CTG Cuts at: 3456 5028 3456 Size: 1572 4141 ApaLI G'TGCA_C Cuts at: 3770 4926 3770 Size: 1156 4557 ApoI r'AATT_y Cuts at: 1493 2363 2544 3022 3248 3400 5509 1493 Size: 870 181 478 226 152 2109 1697 Fragments arranged by size: 2109 1697 870 478 226 181 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 3038 1870 Size: 73 1095 4545 Fragments arranged by size: 4545 1095 73 BamHI G'GATC_C Cuts at: 2984 3043 3410 2984 Size: 59 367 5287 Fragments arranged by size: 5287 367 59 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3034 4489 618 Size: 325 217 627 1247 1455 1842 Fragments arranged by size: 1842 1455 1247 627 325 217 BanII G_rGCy'C Cuts at: 1391 2246 2532 2979 3032 1391 Size: 855 286 447 53 4072 Fragments arranged by size: 4072 855 447 286 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2272 928 Size: 971 373 4369 Fragments arranged by size: 4369 971 373 BbvCI CC'TCA_GC Cuts at: 2142 2996 2142 Size: 854 4859 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2375 2409 3904 3938 1903 Size: 34 438 34 1495 34 3678 Fragments arranged by size: 3678 1495 438 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3605 4815 3605 Size: 1210 4503 BclI T'GATC_A Cuts at: 1548 2698 2784 1548 Size: 1150 86 4477 Fragments arranged by size: 4477 1150 86 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2232 2241 4324 Size: 107 122 71 631 605 560 9 2083 Cuts at: 4324 136 Size: 1525 Fragments arranged by size: 2083 1525 631 605 560 122 107 71 9 BglII A'GATC_T Cuts at: 2121 5708 2121 Size: 3587 2126 BlpI GC'TnA_GC Cuts at: 3130 3130 Size: 5713 Bme1580I G_kGCm'C Cuts at: 1790 3774 4930 1790 Size: 1984 1156 2573 Fragments arranged by size: 2573 1984 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5713 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2635 3444 4402 Size: 416 53 83 399 24 1218 809 958 Cuts at: 4402 442 Size: 1753 Fragments arranged by size: 1753 1218 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4373 1239 Size: 3134 2579 Bpu10I CC'TnA_GC Cuts at: 2142 2996 2142 Size: 854 4859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2963 3838 4703 5001 2963 Size: 875 865 298 3675 Fragments arranged by size: 3675 875 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2913 4376 882 Size: 617 1414 1463 2219 Fragments arranged by size: 2219 1463 1414 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 493 Size: 711 2300 2702 Fragments arranged by size: 2702 2300 711 BsaBI GATnn'nnATC Cuts at: 5707 5707 Size: 5713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3520 3902 4537 275 Size: 53 83 186 936 1987 382 635 1451 Fragments arranged by size: 1987 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2050 4146 4544 4818 4965 5376 2050 Size: 2096 398 274 147 411 2387 Fragments arranged by size: 2387 2096 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5488 Fragments arranged by size: 5488 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2199 1186 Size: 1013 4700 BseYI C'CCAG_C Cuts at: 4916 5412 4916 Size: 496 5217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5140 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3163 3924 4952 1540 Size: 275 186 1162 761 1028 2301 Fragments arranged by size: 2301 1162 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3774 3859 4930 3774 Size: 85 1071 4557 Fragments arranged by size: 4557 1071 85 BsmI GAATG_Cn' Cuts at: 3224 3317 3224 Size: 93 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2246 2532 2979 3032 3774 3859 4930 Size: 399 456 286 447 53 742 85 1071 Cuts at: 4930 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 456 447 399 286 85 53 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 2188 3602 5332 2188 Size: 1414 1730 2569 Fragments arranged by size: 2569 1730 1414 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3068 844 Size: 2224 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3600 1394 Size: 429 1777 3507 Fragments arranged by size: 3507 1777 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4208 4382 65 Size: 1183 2960 174 1396 Fragments arranged by size: 2960 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4357 4544 1700 Size: 110 2547 187 2869 Fragments arranged by size: 2869 2547 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 3773 4785 3773 Size: 1012 4701 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5713 BstBI TT'CG_AA Cuts at: 4517 4517 Size: 5713 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5713 BstXI CCAn_nnnn'nTGG Cuts at: 2246 2246 Size: 5713 BstYI r'GATC_y Cuts at: 1972 2121 2984 3043 3410 3795 3812 5264 5350 Size: 149 863 59 367 385 17 1452 86 Cuts at: 5350 5362 5708 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 863 385 367 346 149 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2172 2172 Size: 5713 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4974 Fragments arranged by size: 4974 683 56 BtsI GCAGTG_nn' Cuts at: 2954 3229 4023 4043 2954 Size: 275 794 20 4624 Fragments arranged by size: 4624 794 275 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5678 DraI TTT'AAA Cuts at: 3017 3017 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3160 4049 5678 Size: 54 1425 50 275 18 1330 889 1629 Cuts at: 5678 8 Size: 43 Fragments arranged by size: 1629 1425 1330 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3160 1537 Size: 275 1348 4090 Fragments arranged by size: 4090 1348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 2628 3643 1353 Size: 1053 222 1015 3423 Fragments arranged by size: 3423 1053 1015 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4300 4674 4820 387 Size: 1064 138 2711 374 146 1280 Fragments arranged by size: 2711 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3776 4373 5160 5428 1239 Size: 393 2144 597 787 268 1524 Fragments arranged by size: 2144 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2357 2357 Size: 5713 EcoRI G'AATT_C Cuts at: 3022 3400 3022 Size: 378 5335 EcoRV GAT'ATC Cuts at: 2627 2627 Size: 5713 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2333 2498 2576 2333 Size: 165 78 5470 Fragments arranged by size: 5470 165 78 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 4860 1678 Size: 3182 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 4354 4386 4428 Size: 32 135 32 534 32 2216 32 42 Cuts at: 4428 4460 1373 Size: 32 2626 Fragments arranged by size: 2626 2216 534 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3057 3303 669 Size: 988 281 1119 246 3079 Fragments arranged by size: 3079 1119 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 3312 3312 Size: 5713 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4827 5011 1305 Size: 3522 184 2007 Fragments arranged by size: 3522 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3891 Fragments arranged by size: 3891 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3671 4030 4189 518 Size: 648 2505 359 159 2042 Fragments arranged by size: 2505 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2678 3129 3806 4954 5199 1606 Size: 1072 451 677 1148 245 2120 Fragments arranged by size: 2120 1148 1072 677 451 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5161 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 3071 4616 1132 Size: 97 1842 1545 2229 Fragments arranged by size: 2229 1842 1545 97 PasI CC'CwG_GG Cuts at: 1348 2239 2923 1348 Size: 891 684 4138 Fragments arranged by size: 4138 891 684 PciI A'CATG_T Cuts at: 4612 4612 Size: 5713 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 1108 Size: 1539 4174 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2357 2357 Size: 5713 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PstI C_TGCA'G Cuts at: 830 3065 830 Size: 2235 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 PvuII CAG'CTG Cuts at: 2678 2678 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SapI GCTCTTCn'nnn_ Cuts at: 2406 2406 Size: 5713 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SfcI C'TryA_G Cuts at: 826 1045 3061 4196 4877 5068 826 Size: 219 2016 1135 681 191 1471 Fragments arranged by size: 2016 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 2978 3817 4718 4980 820 Size: 197 926 633 402 839 901 262 1553 Fragments arranged by size: 1553 926 901 839 633 402 262 197 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3071 1132 Size: 1939 3774 SspI AAT'ATT Cuts at: 5 52 3637 5 Size: 47 3585 2081 Fragments arranged by size: 3585 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5713 StyI C'CwwG_G Cuts at: 513 1065 3152 513 Size: 552 2087 3074 Fragments arranged by size: 3074 2087 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3740 3757 3910 4095 4540 325 Size: 1255 2160 17 153 185 445 1498 Fragments arranged by size: 2160 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2476 3959 5671 Size: 274 80 33 51 1443 499 1483 1712 Cuts at: 5671 96 Size: 138 Fragments arranged by size: 1712 1483 1443 499 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 2313 2316 2556 3842 2313 Size: 3 240 1286 4184 Fragments arranged by size: 4184 1286 240 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI