(Circular) (Six-base) MAPSORT of: pFN21AB7377.seq Check: 3239 from: 1 to: 6052 pFN21AB7377 6052 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2258 2695 3030 2258 Size: 437 335 5280 Fragments arranged by size: 5280 437 335 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2784 3395 1937 Size: 847 611 4594 Fragments arranged by size: 4594 847 611 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4115 5499 5767 1632 Size: 2483 1384 268 1917 Fragments arranged by size: 2483 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 4951 5928 1203 Size: 3748 977 1327 Fragments arranged by size: 3748 1327 977 AgeI A'CCGG_T Cuts at: 4883 4883 Size: 6052 AhdI GACnn_n'nnGTC Cuts at: 4781 4781 Size: 6052 AleI CACnn'nnGTG Cuts at: 1166 2655 2681 1166 Size: 1489 26 4537 Fragments arranged by size: 4537 1489 26 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6052 AlwNI CAG_nnn'CTG Cuts at: 3058 3795 5367 3058 Size: 737 1572 3743 Fragments arranged by size: 3743 1572 737 ApaI G_GGCC'C Cuts at: 2057 2057 Size: 6052 ApaLI G'TGCA_C Cuts at: 2596 4109 5265 2596 Size: 1513 1156 3383 Fragments arranged by size: 3383 1513 1156 ApoI r'AATT_y Cuts at: 1493 3361 3587 3739 5848 1493 Size: 1868 226 152 2109 1697 Fragments arranged by size: 2109 1868 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2875 3280 3377 1870 Size: 73 932 405 97 4545 Fragments arranged by size: 4545 932 405 97 73 BamHI G'GATC_C Cuts at: 2198 3382 3749 2198 Size: 1184 367 4501 Fragments arranged by size: 4501 1184 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2329 2646 2989 3373 4828 Size: 325 217 627 542 317 343 384 1455 Cuts at: 4828 618 Size: 1842 Fragments arranged by size: 1842 1455 627 542 384 343 325 317 217 BanII G_rGCy'C Cuts at: 1391 2057 2064 2925 3371 1391 Size: 666 7 861 446 4072 Fragments arranged by size: 4072 861 666 446 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2704 3230 928 Size: 971 805 526 3750 Fragments arranged by size: 3750 971 805 526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4243 4277 1903 Size: 34 50 34 2222 34 3678 Fragments arranged by size: 3678 2222 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2496 3944 5154 2496 Size: 1448 1210 3394 Fragments arranged by size: 3394 1448 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4663 136 Size: 107 122 71 631 605 337 2654 1525 Fragments arranged by size: 2654 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2271 6047 2271 Size: 3776 2276 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2057 2334 2600 2649 4113 5269 1790 Size: 267 277 266 49 1464 1156 2573 Fragments arranged by size: 2573 1464 1156 277 267 266 49 BmgBI CAC'GTC Cuts at: 1318 2551 1318 Size: 1233 4819 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2530 3783 4741 Size: 416 53 83 399 24 1113 1253 958 Cuts at: 4741 442 Size: 1753 Fragments arranged by size: 1753 1253 1113 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2218 2218 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2113 2288 2794 2936 3227 4712 1239 Size: 809 65 175 506 142 291 1485 2579 Fragments arranged by size: 2579 1485 809 506 291 175 142 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3025 4177 5042 5340 3025 Size: 1152 865 298 3737 Fragments arranged by size: 3737 1152 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4715 882 Size: 617 3216 2219 Fragments arranged by size: 3216 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2611 3843 493 Size: 711 1407 1232 2702 Fragments arranged by size: 2702 1407 1232 711 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2445 3859 4241 4876 Size: 53 83 186 936 912 1414 382 635 Cuts at: 4876 275 Size: 1451 Fragments arranged by size: 1451 1414 936 912 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4485 4883 5157 5304 5715 4485 Size: 398 274 147 411 4822 Fragments arranged by size: 4822 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2813 2843 1178 Size: 30 165 30 1410 30 4387 Fragments arranged by size: 4387 1410 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2341 2933 2999 1186 Size: 1155 592 66 4239 Fragments arranged by size: 4239 1155 592 66 BseYI C'CCAG_C Cuts at: 2050 2308 2825 2998 3127 3344 5255 5751 2050 Size: 258 517 173 129 217 1911 496 2351 Fragments arranged by size: 2351 1911 517 496 258 217 173 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2191 3053 1163 Size: 573 455 862 4162 Fragments arranged by size: 4162 862 573 455 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2600 4113 4198 5269 2106 Size: 494 1513 85 1071 2889 Fragments arranged by size: 2889 1513 1071 494 85 BsmI GAATG_Cn' Cuts at: 2777 3563 3656 2777 Size: 786 93 5173 Fragments arranged by size: 5173 786 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2064 2106 2334 2600 2649 2925 Size: 399 267 7 42 228 266 49 276 Cuts at: 2925 3371 4113 4198 5269 1391 Size: 446 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 446 399 276 267 266 228 85 49 42 7 BspEI T'CCGG_A Cuts at: 5715 5715 Size: 6052 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2258 2313 2695 3030 3407 844 Size: 1414 55 382 335 377 3489 Fragments arranged by size: 3489 1414 382 377 335 55 BsrBI CCG'CTC Cuts at: 1394 1823 3939 1394 Size: 429 2116 3507 Fragments arranged by size: 3507 2116 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2593 4547 4721 65 Size: 1183 1345 1954 174 1396 Fragments arranged by size: 1954 1396 1345 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4696 4883 1700 Size: 110 2886 187 2869 Fragments arranged by size: 2886 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 2681 4112 5124 2681 Size: 1431 1012 3609 Fragments arranged by size: 3609 1431 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2514 794 Size: 1720 4332 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6052 BstXI CCAn_nnnn'nTGG Cuts at: 2657 2657 Size: 6052 BstYI r'GATC_y Cuts at: 1972 2198 2271 3382 3749 4134 4151 5603 5689 Size: 226 73 1111 367 385 17 1452 86 Cuts at: 5689 5701 6047 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1111 385 367 346 226 86 73 17 12 BstZ17I GTA'TAC Cuts at: 2785 2785 Size: 6052 BtgI C'CryG_G Cuts at: 513 1065 2076 2404 2641 2740 513 Size: 552 1011 328 237 99 3825 Fragments arranged by size: 3825 1011 552 328 237 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5313 Fragments arranged by size: 5313 683 56 BtsI GCAGTG_nn' Cuts at: 2045 2046 3568 4362 4382 2045 Size: 1 1522 794 20 3715 Fragments arranged by size: 3715 1522 794 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 3356 3356 Size: 6052 DraIII CAC_nnn'GTG Cuts at: 2169 2524 2169 Size: 355 5697 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2458 5059 809 Size: 507 1142 2601 1802 Fragments arranged by size: 2601 1802 1142 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2397 2790 Size: 54 1425 50 275 18 360 207 393 Cuts at: 2790 3499 4388 6017 8 Size: 709 889 1629 43 Fragments arranged by size: 1629 1425 889 709 393 360 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 3982 1353 Size: 1233 1396 3423 Fragments arranged by size: 3423 1396 1233 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2855 4639 5013 5159 387 Size: 1064 138 1266 1784 374 146 1280 Fragments arranged by size: 1784 1280 1266 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2113 2288 2794 2936 3227 4115 Size: 393 416 65 175 506 142 291 888 Cuts at: 4115 4712 5499 5767 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 888 787 597 506 416 393 291 268 175 142 65 EcoO109I rG'GnC_Cy Cuts at: 2283 2467 2627 2670 3284 2283 Size: 184 160 43 614 5051 Fragments arranged by size: 5051 614 184 160 43 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2740 2914 2740 Size: 174 5878 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 5199 1678 Size: 3521 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4693 4725 4767 4799 1373 Size: 32 135 32 3121 32 42 32 2626 Fragments arranged by size: 3121 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3396 3642 669 Size: 988 281 1458 246 3079 Fragments arranged by size: 3079 1458 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2745 5166 5350 1305 Size: 1440 2421 184 2007 Fragments arranged by size: 2421 2007 1440 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 2792 10 Size: 54 1425 343 567 393 3270 Fragments arranged by size: 3270 1425 567 393 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2655 2681 2792 4010 4369 4528 518 Size: 648 1489 26 111 1218 359 159 2042 Fragments arranged by size: 2042 1489 1218 648 359 159 111 26 MspA1I CmG'CkG Cuts at: 1606 2175 2537 2850 3127 3468 4145 5293 5538 Size: 569 362 313 277 341 677 1148 245 Cuts at: 5538 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 569 362 341 313 277 245 NcoI C'CATG_G Cuts at: 513 1065 2076 2404 513 Size: 552 1011 328 4161 Fragments arranged by size: 4161 1011 552 328 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 2363 2396 3410 4955 1132 Size: 97 1134 33 1014 1545 2229 Fragments arranged by size: 2229 1545 1134 1014 97 33 PasI CC'CwG_GG Cuts at: 1348 2057 1348 Size: 709 5343 PciI A'CATG_T Cuts at: 4951 4951 Size: 6052 PflMI CCAn_nnn'nTGG Cuts at: 1108 2077 1108 Size: 969 5083 PfoI T'CCnGG_A Cuts at: 2901 2901 Size: 6052 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 3284 2467 Size: 817 5235 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 3622 3622 Size: 6052 PspOMI G'GGCC_C Cuts at: 2053 2053 Size: 6052 PstI C_TGCA'G Cuts at: 830 2255 2706 3404 830 Size: 1425 451 698 3478 Fragments arranged by size: 3478 1425 698 451 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 2175 3127 2175 Size: 952 5100 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SanDI GG'GwC_CC Cuts at: 3284 3284 Size: 6052 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SexAI A'CCwGG_T Cuts at: 2883 2883 Size: 6052 SfcI C'TryA_G Cuts at: 826 1045 2251 2702 3400 4535 5216 5407 826 Size: 219 1206 451 698 1135 681 191 1471 Fragments arranged by size: 1471 1206 1135 698 681 451 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 2877 3379 1872 Size: 1005 502 4545 Fragments arranged by size: 4545 1005 502 SmlI C'TyrA_G Cuts at: 820 1017 1943 3040 4156 5057 5319 820 Size: 197 926 1097 1116 901 262 1553 Fragments arranged by size: 1553 1116 1097 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 3410 1132 Size: 2278 3774 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 2753 1517 Size: 1236 4816 StyI C'CwwG_G Cuts at: 513 1065 2076 2404 3491 513 Size: 552 1011 328 1087 3074 Fragments arranged by size: 3074 1087 1011 552 328 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2512 4079 4096 4249 4434 4879 325 Size: 1255 932 1567 17 153 185 445 1498 Fragments arranged by size: 1567 1498 1255 932 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4298 6010 96 Size: 274 80 33 51 1443 2321 1712 138 Fragments arranged by size: 2321 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2190 2222 2995 3027 6012 6044 2190 Size: 32 773 32 2985 32 2198 Fragments arranged by size: 2985 2198 773 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6052 XmaI C'CCGG_G Cuts at: 1870 2875 3377 1870 Size: 1005 502 4545 Fragments arranged by size: 4545 1005 502 XmnI GAAnn'nnTTC Cuts at: 4181 4181 Size: 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI