(Circular) (Six-base) MAPSORT of: pFN21AB7376.seq Check: 2693 from: 1 to: 5695 pFN21AB7376 5695 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2103 2348 3758 5142 5410 1632 Size: 471 245 1410 1384 268 1917 Fragments arranged by size: 1917 1410 1384 471 268 245 AfeI AGC'GCT Cuts at: 2205 2205 Size: 5695 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 2272 3438 5010 2272 Size: 1166 1572 2957 Fragments arranged by size: 2957 1572 1166 ApaLI G'TGCA_C Cuts at: 2965 3752 4908 2965 Size: 787 1156 3752 Fragments arranged by size: 3752 1156 787 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2729 3020 1870 Size: 73 786 291 4545 Fragments arranged by size: 4545 786 291 73 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3016 4471 618 Size: 325 217 627 1229 1455 1842 Fragments arranged by size: 1842 1455 1229 627 325 217 BanII G_rGCy'C Cuts at: 1391 3014 1391 Size: 1623 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2231 928 Size: 971 332 4392 Fragments arranged by size: 4392 971 332 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3886 3920 1903 Size: 34 1949 34 3678 Fragments arranged by size: 3678 1949 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 2952 1548 Size: 1404 4291 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2198 4306 136 Size: 107 122 71 631 605 526 2108 1525 Fragments arranged by size: 2108 1525 631 605 526 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2969 3756 4912 1790 Size: 1179 787 1156 2573 Fragments arranged by size: 2573 1179 1156 787 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5695 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2791 4355 1239 Size: 1085 467 1564 2579 Fragments arranged by size: 2579 1564 1085 467 Bpu10I CC'TnA_GC Cuts at: 2894 2894 Size: 5695 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2165 2895 3820 4685 4983 2165 Size: 730 925 865 298 2877 Fragments arranged by size: 2877 925 865 730 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2725 2966 4358 882 Size: 617 1226 241 1392 2219 Fragments arranged by size: 2219 1392 1226 617 241 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 3884 4519 275 Size: 53 83 186 936 1969 382 635 1451 Fragments arranged by size: 1969 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2588 2747 2861 1186 Size: 1207 195 159 114 4020 Fragments arranged by size: 4020 1207 195 159 114 BseYI C'CCAG_C Cuts at: 2118 4898 5394 2118 Size: 2780 496 2419 Fragments arranged by size: 2780 2419 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3145 3906 4934 1540 Size: 275 186 1144 761 1028 2301 Fragments arranged by size: 2301 1144 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2969 3756 3841 4912 2969 Size: 787 85 1071 3752 Fragments arranged by size: 3752 1071 787 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 BsmBI CGTCTCn'nnnn_ Cuts at: 2158 2539 2158 Size: 381 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2969 3014 3756 3841 4912 1391 Size: 399 1179 45 742 85 1071 2174 Fragments arranged by size: 2174 1179 1071 742 399 85 45 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2257 3050 844 Size: 1413 793 3489 Fragments arranged by size: 3489 1413 793 BsrBI CCG'CTC Cuts at: 1394 1823 3582 1394 Size: 429 1759 3507 Fragments arranged by size: 3507 1759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2653 4339 4526 1700 Size: 110 843 1686 187 2869 Fragments arranged by size: 2869 1686 843 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5695 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstYI r'GATC_y Cuts at: 1972 2028 2736 3025 3392 3777 3794 5246 5332 Size: 56 708 289 367 385 17 1452 86 Cuts at: 5332 5344 5690 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 708 385 367 346 289 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 2219 2219 Size: 5695 BtgI C'CryG_G Cuts at: 513 1065 2004 2538 2617 513 Size: 552 939 534 79 3591 Fragments arranged by size: 3591 939 552 534 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 2969 3211 4005 4025 2969 Size: 242 794 20 4639 Fragments arranged by size: 4639 794 242 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2887 2999 2887 Size: 112 5583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2541 3142 4031 Size: 54 1425 50 275 18 711 601 889 Cuts at: 4031 5660 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 711 601 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 2940 3625 1353 Size: 860 727 685 3423 Fragments arranged by size: 3423 860 727 685 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2651 4282 4656 4802 387 Size: 1064 138 1062 1631 374 146 1280 Fragments arranged by size: 1631 1280 1064 1062 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2103 2324 2348 2791 3758 4355 5142 Size: 393 471 221 24 443 967 597 787 Cuts at: 5142 5410 1239 Size: 268 1524 Fragments arranged by size: 1524 967 787 597 471 443 393 268 221 24 EcoNI CCTnn'n_nnAGG Cuts at: 2570 2594 2636 2744 2570 Size: 24 42 108 5521 Fragments arranged by size: 5521 108 42 24 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2050 2050 Size: 5695 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2207 2525 4842 1678 Size: 529 318 2317 2531 Fragments arranged by size: 2531 2317 529 318 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373 Size: 32 135 32 2764 32 42 32 2626 Fragments arranged by size: 2764 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 2767 748 Size: 2019 3676 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3873 Fragments arranged by size: 3873 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3653 4012 4171 518 Size: 648 2487 359 159 2042 Fragments arranged by size: 2487 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2134 2496 2540 2619 2685 2709 2826 Size: 516 12 362 44 79 66 24 117 Cuts at: 2826 3111 3788 4936 5181 1606 Size: 285 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 516 362 285 245 117 79 66 44 24 12 NaeI GCC'GGC Cuts at: 2655 2655 Size: 5695 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4204 Fragments arranged by size: 4204 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NgoMIV G'CCGG_C Cuts at: 2653 2653 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 2366 2407 3053 4598 1132 Size: 97 1137 41 646 1545 2229 Fragments arranged by size: 2229 1545 1137 646 97 41 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5695 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5695 PfoI T'CCnGG_A Cuts at: 2772 2772 Size: 5695 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2170 1613 Size: 557 5138 PsiI TTA'TAA Cuts at: 2069 3265 2069 Size: 1196 4499 PstI C_TGCA'G Cuts at: 830 2139 3047 830 Size: 1309 908 3478 Fragments arranged by size: 3478 1309 908 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 PvuII CAG'CTG Cuts at: 2122 2134 2709 2826 2122 Size: 12 575 117 4991 Fragments arranged by size: 4991 575 117 12 SacII CC_GC'GG Cuts at: 2541 2620 2541 Size: 79 5616 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SapI GCTCTTCn'nnn_ Cuts at: 2213 2213 Size: 5695 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 2135 3043 4178 4859 5050 826 Size: 219 1090 908 1135 681 191 1471 Fragments arranged by size: 1471 1135 1090 908 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 3022 1872 Size: 1150 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 2910 3799 4700 4962 820 Size: 197 926 201 766 889 901 262 1553 Fragments arranged by size: 1553 926 901 889 766 262 201 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 2366 3053 1132 Size: 1234 687 3774 Fragments arranged by size: 3774 1234 687 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2600 2642 2892 1517 Size: 1083 42 250 4320 Fragments arranged by size: 4320 1083 250 42 StyI C'CwwG_G Cuts at: 513 1065 2004 2447 3134 513 Size: 552 939 443 687 3074 Fragments arranged by size: 3074 939 687 552 443 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3722 3739 3892 4077 4522 325 Size: 1255 2142 17 153 185 445 1498 Fragments arranged by size: 2142 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2232 2795 3941 Size: 274 80 33 51 1443 255 563 1146 Cuts at: 3941 5653 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1146 563 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2713 2713 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 3020 1870 Size: 1150 4545 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI