Restriction map for
pFN21AB7372
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# NO CUT ENZYMES ( 165 enzymes) #
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Acc65I AccII AccIII AciI AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MfeI MluI MroI MslI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 610 GACGTC GACGT^C
AccI 739 GTAGAC GT^[AC][GT]AC
AcyI 610 GACGTC G[GA]^CG[CT]C
AfaI 286 GTAC GT^AC
AspHI 10 GAGCAC G[AT]GC[AT]^C
AvaII 66 GGTCC G^G[AT]CC
BalI 39 TGGCCA TGG^CCA
BanI 128 GGCACC G^G[CT][GA]CC
BbsI 664 GTCTTC GAAGAC(2/6)
BglII 599 AGATCT A^GATCT
BmyI 10 GAGCAC G[AGT]GC[ACT]^C
BpuAI 664 GTCTTC GAAGAC(2/6)
BsaAI 639 CACGTA [CT]AC^GT[GA]
BsaBI 222 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 610 GACGTC G[GA]^CG[CT]C
BsiHKAI 10 GAGCAC G[AT]GC[AT]^C
Bsp1286I 10 GAGCAC G[AGT]GC[ACT]^C
BssSI 670 CACGAG CACGAG(-5/-1)
BstEII 150 GGTGACC G^GT[ACGT]ACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
EaeI 39 TGGCCA [CT]^GGCC[GA]
EcoNI 767 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
Hin1I 610 GACGTC G[GA]^CG[CT]C
HinfI 541 GAGTC G^A[ACGT]TC
HphI 150 GGTGA GGTGA(8/7)
MamI 222 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 39 TGGCCA TGG^CCA
MscI 39 TGGCCA TGG^CCA
MseI 395 TTAA T^TAA
MspA1I 100 CAGCTG C[AC]G^C[GT]G
NcoI 428 CCATGG C^CATGG
PleI 541 GAGTC GAGTC(4/5)
PvuII 100 CAGCTG CAG^CTG
RsaI 286 GTAC GT^AC
SfcI 534 CTACAG C^T[GA][CT]AG
SmlI 745 CTCAAG C^T[CT][GA]AG
StuI 775 AGGCCT AGG^CCT
Tru9I 395 TTAA T^TAA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 638 ACACGT A^C[GA][CT]GT
715 ACGTGT
AlwI 227 GGATC GGATC(4/5)
626 GGATC
BfaI 217 CTAG C^TAG
283 CTAG
BsmFI 485 GGGAC GGGAC(10/14)
719 GTCCC
BstYI 227 GGATCT [GA]^GATC[CT]
599 AGATCT
EarI 451 CTCTTC CTCTTC(1/4)
619 GAAGAG
EcoT22I 22 ATGCAT ATGCA^T
52 ATGCAT
Ksp632I 451 CTCTTC CTCTTC(1/4)
619 GAAGAG
MaeI 217 CTAG C^TAG
283 CTAG
MflI 227 GGATCT [GA]^GATC[CT]
599 AGATCT
NsiI 22 ATGCAT ATGCA^T
52 ATGCAT
TaqI 8 TCGA T^CGA
813 TCGA
Tsp45I 151 GTGAC ^GT[GC]AC
244 GTCAC
TthHB8I 8 TCGA T^CGA
813 TCGA
XhoII 227 GGATCT [GA]^GATC[CT]
599 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI BbvI BsaJI BsgI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII ItaI
MaeII MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
SphI StyI TseI TspRI