(Circular) (Six-base) MAPSORT of: pFN21AB7372.seq Check: 6982 from: 1 to: 5590 pFN21AB7372 5590 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2619 3400 278 Size: 53 83 186 936 1083 781 2468 Fragments arranged by size: 2468 1083 936 781 186 83 53 AccI GT'mk_AC Cuts at: 1937 2745 2933 1937 Size: 808 188 4594 Fragments arranged by size: 4594 808 188 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3653 5037 5305 1632 Size: 2021 1384 268 1917 Fragments arranged by size: 2021 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 2643 2720 4489 5466 1203 Size: 1440 77 1769 977 1327 Fragments arranged by size: 1769 1440 1327 977 77 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 3333 4905 3333 Size: 1572 4018 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2133 2911 4366 618 Size: 325 217 627 346 778 1455 1842 Fragments arranged by size: 1842 1455 778 627 346 325 217 BanII G_rGCy'C Cuts at: 1391 2909 1391 Size: 1518 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2662 928 Size: 971 763 3856 Fragments arranged by size: 3856 971 763 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 1548 Size: 5590 BfrBI ATG'CAT Cuts at: 2029 2059 2029 Size: 30 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4201 136 Size: 107 122 71 631 605 2529 1525 Fragments arranged by size: 2529 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2604 5585 2604 Size: 2981 2609 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 3651 4807 1790 Size: 1861 1156 2573 Fragments arranged by size: 2573 1861 1156 BmgBI CAC'GTC Cuts at: 1318 2788 1318 Size: 1470 4120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3321 4279 442 Size: 416 53 83 399 24 1904 958 1753 Fragments arranged by size: 1904 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4250 1239 Size: 3011 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2735 3715 4580 4878 2735 Size: 980 865 298 3447 Fragments arranged by size: 3447 980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4253 882 Size: 617 2754 2219 Fragments arranged by size: 2754 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2646 3381 493 Size: 711 1442 735 2702 Fragments arranged by size: 2702 1442 735 711 BsaBI GATnn'nnATC Cuts at: 2231 5584 2231 Size: 3353 2237 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2616 3397 3779 4414 Size: 53 83 186 936 1083 781 382 635 Cuts at: 4414 275 Size: 1451 Fragments arranged by size: 1451 1083 936 781 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2126 2156 1178 Size: 30 165 30 723 30 4612 Fragments arranged by size: 4612 723 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5590 BseYI C'CCAG_C Cuts at: 2103 4793 5289 2103 Size: 2690 496 2404 Fragments arranged by size: 2690 2404 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 2189 2529 1163 Size: 573 402 51 340 4224 Fragments arranged by size: 4224 573 402 340 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3801 4829 1540 Size: 275 186 1039 761 1028 2301 Fragments arranged by size: 2301 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2019 3651 3736 4807 2019 Size: 1632 85 1071 2802 Fragments arranged by size: 2802 1632 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2909 3651 3736 4807 1391 Size: 399 229 890 742 85 1071 2174 Fragments arranged by size: 2174 1071 890 742 399 229 85 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2945 844 Size: 2101 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4085 4259 65 Size: 1183 2837 174 1396 Fragments arranged by size: 2837 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4234 4421 1700 Size: 110 2424 187 2869 Fragments arranged by size: 2869 2424 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 2675 3650 4662 2675 Size: 975 1012 3603 Fragments arranged by size: 3603 1012 975 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5590 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 2155 1184 Size: 971 4619 BstYI r'GATC_y Cuts at: 1972 2232 2604 2920 3287 3672 3689 5141 5227 Size: 260 372 316 367 385 17 1452 86 Cuts at: 5227 5239 5585 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 372 367 346 316 260 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2041 2433 2689 513 Size: 552 976 392 256 3414 Fragments arranged by size: 3414 976 552 392 256 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2068 487 Size: 683 56 842 4009 Fragments arranged by size: 4009 842 683 56 BtsI GCAGTG_nn' Cuts at: 2126 3106 3900 3920 2126 Size: 980 794 20 3796 Fragments arranged by size: 3796 980 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2023 2058 2076 2111 2341 2376 529 Size: 35 1459 35 18 35 230 35 3743 Fragments arranged by size: 3743 1459 230 35 35 35 35 18 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 3037 3926 Size: 54 1425 50 275 18 214 993 889 Cuts at: 3926 5555 8 Size: 1629 43 Fragments arranged by size: 1629 1425 993 889 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2462 2619 3520 1353 Size: 1109 157 901 3423 Fragments arranged by size: 3423 1109 901 157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3653 4250 5037 5305 1239 Size: 393 2021 597 787 268 1524 Fragments arranged by size: 2021 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2776 2776 Size: 5590 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 4737 1678 Size: 3059 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373 Size: 32 135 32 2659 32 42 32 2626 Fragments arranged by size: 2659 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2046 10 Size: 54 1425 343 214 3554 Fragments arranged by size: 3554 1425 343 214 54 MslI CAynn'nnrTG Cuts at: 518 1166 3548 3907 4066 518 Size: 648 2382 359 159 2042 Fragments arranged by size: 2382 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 3006 3683 4831 5076 1606 Size: 501 899 677 1148 245 2120 Fragments arranged by size: 2120 1148 899 677 501 245 NcoI C'CATG_G Cuts at: 513 1065 2433 513 Size: 552 1368 3670 Fragments arranged by size: 3670 1368 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NsiI A_TGCA'T Cuts at: 2031 2061 2031 Size: 30 5560 NspI r_CATG'y Cuts at: 1132 1229 2029 2033 2063 2557 2948 4493 1132 Size: 97 800 4 30 494 391 1545 2229 Fragments arranged by size: 2229 1545 800 494 391 97 30 4 PasI CC'CwG_GG Cuts at: 1348 2772 1348 Size: 1424 4166 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 2316 3160 2316 Size: 844 4746 PstI C_TGCA'G Cuts at: 830 2942 830 Size: 2112 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2107 2107 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2539 2938 4073 4754 4945 826 Size: 219 1494 399 1135 681 191 1471 Fragments arranged by size: 1494 1471 1135 681 399 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2750 3694 4595 4857 820 Size: 197 926 807 944 901 262 1553 Fragments arranged by size: 1553 944 926 901 807 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2029 2033 2063 2557 2948 1132 Size: 897 4 30 494 391 3774 Fragments arranged by size: 3774 897 494 391 30 4 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 2782 1517 Size: 1265 4325 StyI C'CwwG_G Cuts at: 513 1065 2091 2303 2433 2860 3029 513 Size: 552 1026 212 130 427 169 3074 Fragments arranged by size: 3074 1026 552 427 212 169 130 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2151 3617 3634 3787 3972 4417 325 Size: 1255 571 1466 17 153 185 445 1498 Fragments arranged by size: 1498 1466 1255 571 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2290 3836 5548 Size: 274 80 33 51 1443 313 1546 1712 Cuts at: 5548 96 Size: 138 Fragments arranged by size: 1712 1546 1443 313 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 5550 5582 2126 Size: 32 3392 32 2134 Fragments arranged by size: 3392 2134 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2617 3398 276 Size: 53 83 186 936 1083 781 2468 Fragments arranged by size: 2468 1083 936 781 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI